B-cell epitope of tnf-α protein, multi-antigen peptide containing this epitope and application

Abstract

The invention provides TNF-Alpha protein B cell epitopes, an 8-branch-peptide-conformation multiple antigen peptide synthesized on the basis of the TNF-Alpha protein B cell epitopes combining with an MAP (multiple antigen peptide) scheme, and application of the multiple antigen peptide in the preparation of ulcerative colitis vaccines. The MAP scheme is creatively applied to the field of immunological research on small molecular autoantigens, and human TNF-Alpha protein B cell dominant epitopes are screened out through a large amount of tests. By using the MAP scheme on such basis, 8-branch peptide conformation with lysine as the core matrix is acquired, the 8-branch-peptide-conformation multiple antigen peptide belongs to autoantigen epitope vaccines, and accordingly the 8-branch-peptide-conformation multiple antigen peptide is convenient in administration, few in administration times and capable of inducing antibody generation by small-quantity injection; the multiple antigen peptide is also good in safety and tolerance, capable of achieving both preventing and treating, and the like; an important theoretical basis is hopefully provided for the research and development of autoantigen MAP vaccines, and a new through can be provided for the clinical treatment of ulcerative colitis.

Description

technical field [0001] The invention belongs to the field of immunology and gastroenterology, and specifically relates to a B cell epitope of TNF-α protein, and a multi-antigen peptide in an 8-branched peptide conformation synthesized based on the epitope in combination with the MAP scheme, and in the preparation of ulcerative Use in colitis vaccines. Background technique [0002] TNF-α is a cytokine with complex biological activities. In addition to killing tumor cells in vivo and in vitro, it also has multiple functions such as inducing inflammatory responses, antiviral infection, regulating body immunity, and promoting cell proliferation and activation. However, studies have shown that when TNF-α is overexpressed, it will synergistically produce a variety of pathological injuries, such as inflammatory bowel disease, infectious disease, dyscrasia, etc.; at the same time, TNF-α is also a therapeutic target The human body's own antigens can neutralize the excessive TNF-α in...

AI Technical Summary

Problems solved by technology

Compared with macromolecular antigens, self-antigens have defects such as natural immune tolerance, smaller molecular weight, shorter epitope peptides that are more difficult to screen, and less likely to cause humoral immune responses. Therefore, researchers in this field have never applied the MAP design scheme. self small molecule antigen

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