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A kind of antiviral biological agent prepared by using Cordyceps polysaccharide and its production process

A technology of biological preparations and Cordyceps polysaccharides, applied in the field of biological preparations and their production processes, antiviral biological preparations and their production processes, can solve the problems of unsatisfactory antiviral activity, affecting modified polysaccharides, low antiviral activity, etc., and achieve prevention Effects of viral diseases, low cost, and simple process

Active Publication Date: 2019-05-10
山东仙普爱瑞科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many deficiencies in the current research on sulfated polysaccharides. The degree of substitution of sulfate groups, molecular weight, position of sulfate groups, and branched structure of polysaccharides all affect the activity of modified polysaccharides, making their antiviral activity unsatisfactory.
[0005] The prior art has the following defects: the antiviral activity of Cordyceps polysaccharide and Cordyceps polysaccharide derivatives is relatively low

Method used

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  • A kind of antiviral biological agent prepared by using Cordyceps polysaccharide and its production process
  • A kind of antiviral biological agent prepared by using Cordyceps polysaccharide and its production process
  • A kind of antiviral biological agent prepared by using Cordyceps polysaccharide and its production process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 A process for preparing antiviral biological preparations using Cordyceps polysaccharides

[0029] Step 1 Bacillus subtilis activation

[0030] Bacillus subtilis stored at -80°C was inoculated on solid YPD medium with an inoculation loop, and activated at 37°C for 24 hours.

[0031] The components of the YPD solid medium are as follows: glucose 20 g / L, yeast powder 10 g / L, peptone 20 g / L, agar 20 g / L, and deionized water to make up the balance.

[0032] Step 2 Strain Expansion

[0033] Pick a ring of activated Bacillus subtilis from the plate and inoculate it in a 250mL Erlenmeyer flask containing a strain expansion medium. Each Erlenmeyer flask is equipped with a 50mL strain expansion medium. Shake culture at a constant temperature for 20 hours at a rotational speed of 200 rpm to obtain a seed liquid.

[0034] Wherein the composition of the strain expansion culture medium is as follows: deionized water is used as the medium, glucose 20 g / L, yeast powder 10...

Embodiment 2

[0054] Example 2 Carbon source single factor analysis experiment of fermentation medium

[0055] Adopt the method of embodiment 1 to prepare biological agent, only change the carbon source of the fermentation medium in the fermentation step to carry out embodiment 2-6; The carbon source of the fermentation medium used in embodiment 1 is: 10 / L Cordyceps polysaccharide, 10 g / L glucose;

[0056] The carbon source of the fermentation medium that embodiment 2-6 adopts is shown in Table 2;

[0057] The carbon source of the fermentation medium that table 2 embodiment 2-6 adopts

[0058]

[0059] After testing, the antiviral activity of the biological preparations prepared in Examples 1-6 is shown in Table 3;

[0060] Table 3 The antiviral activity of the biological preparations prepared in Examples 1-6

[0061]

[0062] As can be seen from Tables 2 and 3, Example 2 is a preferred embodiment; the carbon source of the fermentation medium has a greater impact on the antiviral ...

Embodiment 7

[0063] Nitrogen source single factor analysis experiment of embodiment 7 fermentation medium

[0064]Adopt the method of Example 1 to prepare biological preparations, only change the nitrogen source of the fermentation medium in the fermentation step to carry out Examples 7-16; the nitrogen source of the fermentation medium of Example 1 is: 12 g / L peptone, 8 g / L urea;

[0065] The nitrogen source of the fermentation medium that embodiment 7-16 adopts is shown in Table 4;

[0066] The nitrogen source of the fermentation medium that table 4 embodiment 7-16 adopts

[0067]

[0068] After testing, the antiviral activity of the biological preparations prepared in Examples 7-16 is shown in Table 5;

[0069] The antiviral activity of the biological agent prepared in table 5 embodiment 7-16

[0070]

[0071] As can be seen from Tables 4 and 5, Example 13 is a preferred embodiment; the nitrogen source of the fermentation medium is preferably 12 g / L peptone and 8 g / L gluten.

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Abstract

The invention provides an antiviral biological preparation prepared by using cordyceps polysaccharides and a preparing process of the antiviral biological preparation. The antiviral activity of the biological preparation reaches 3.45*10<3>-6.22*10<4>IU / mL. The preparing process includes: activating bacillus subtilis, performing strain expanding culture and fermenting to obtain the biological preparation, wherein a fermentation medium used by the fermenting step comprises 15-25g / L of cordyceps polysaccharides, 8-16g / L of peptone, 4-12g / L of wheat gluten, 0.5-2g / L of iron phosphate and the balance water. The antiviral biological preparation is high in antiviral activity and good in stability.

Description

technical field [0001] The invention relates to a biological preparation and a production process thereof, in particular to an antiviral biological preparation prepared by using Cordyceps polysaccharide and a production process thereof, belonging to the technical field of biological fermentation engineering. Background technique [0002] In recent years, the occurrence and prevalence of infectious diseases have become an important factor hindering the rapid development of animal husbandry in my country, and poultry viral infectious diseases are one of the important factors restricting the development of poultry breeding industry. Mainly amantadine and ribavirin preparations, the clinical curative effect is uncertain, and there are toxic and side effects such as residues, affecting the recovery of egg production rate of laying poultry. Due to the lack of treatment methods and methods, serious chemical residues, and human beings' high attention to the food safety of livestock a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61K31/715A61P31/20C12P19/04C12R1/125
CPCA61K31/715A61K45/00C12P19/04
Inventor 郭海岩刘刚王茂超王兴业韩国英刘东东郝利利郭芹王红军
Owner 山东仙普爱瑞科技股份有限公司
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