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Method for optimizing extraction process of gentiana apiata total flavonoids by response surface method

A technology of extraction process and response surface method, which is applied in the field of optimizing the extraction process of Qinling gentian total flavonoids by response surface method, can solve the problems of inability to examine the interaction of various factors, and it is difficult to examine the interaction relationship, achieving high fitting degree, Calculate the effect of ease

Inactive Publication Date: 2017-03-08
SHAANXI INST OF INT TRADE & COMMERCE
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for optimizing the extraction process of total flavonoids of Gentiana of Qinling Mountains by response surface methodology, aiming to solve the problem that most of the optimization methods of the current extraction process are orthogonal design methods and uniform design methods, and such methods cannot investigate various factors At the same time, it is difficult to investigate the interaction relationship between multiple response values ​​and factors

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  • Method for optimizing extraction process of gentiana apiata total flavonoids by response surface method
  • Method for optimizing extraction process of gentiana apiata total flavonoids by response surface method
  • Method for optimizing extraction process of gentiana apiata total flavonoids by response surface method

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[0016] In order to further understand the content, features and effects of the present invention, the following examples are given, and detailed descriptions are given below with reference to the accompanying drawings.

[0017] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0018] see Figure 1 to Figure 3 :

[0019] In the embodiment of the present invention, under the premise of single factor experiment, with the design principle of central combination experiment, with material-liquid ratio A, ethanol concentration B, ultrasonic time C as the influencing factors of Qinling gentian total flavonoids extraction process, with total flavonoids extraction rate For response values, analysis was performed using Design-Expert 8.0.6Trial software. The experimental conditions and experimental results of the response surface design are shown in Table 1.

[0020] Table 1 Box-Behnken design scheme an...

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Abstract

The invention discloses a method for optimizing an extraction process of gentiana apiata total flavonoids by a response surface method. According to the method, an extraction rate of the gentiana apiata total flavonoids is taken as an index to optimize the extraction process of the gentiana apiata total flavonoid; a regression equation is established through multiple regression and binomial fitting of three factors including extraction time, a solid-to-liquid ratio and ethanol concentration, and a response curved surface map which takes the extraction rate of the gentiana apiata total flavonoids as a response surface value is obtained; a testing result is analyzed to determine optimal extraction process parameters of the gentiana apiata total flavonoids; therefore, the method has a relatively high fitting degree, and can provide reliable references for actual production.

Description

technical field [0001] The invention belongs to the field of extraction of medicinal components, and in particular relates to a method for optimizing the extraction process of Qinling gentian total flavonoids by response surface method. Background technique [0002] Qinling gentian, also known as Zhuling grass, belongs to the Gentianaceae plant. Because it is produced in the Taibai Mountains of the Qinling Mountains, it is named Qinling Gentian, and it is used as medicine with the dried whole herb with flowers. The nature and flavor are "bitter and flat", and it is commonly used by folks to treat digestive tract diseases and gynecological diseases. According to relevant information, the chemical components that have been isolated from Qinling gentian include: flavonoids, triterpenes, secoiridoids, iridoids, polysaccharides, etc. According to relevant literature reports at home and abroad, flavonoids not only have the effects of anti-inflammation and dilating blood vessels,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/515
CPCA61K36/515A61K2236/333A61K2236/53
Inventor 许海燕彭修娟杨长花
Owner SHAANXI INST OF INT TRADE & COMMERCE
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