Method for reducing enzyme usage amount in process of producing acrylamide by virtue of microbiological method

A technology for using acrylamide nitrile hydratase and microbial method, which is applied in the field of reducing the amount of enzyme used in the process of producing acrylamide by microbial method, and can solve the problem of lowering the quality of acrylamide products, reducing the activity of nitrile hydratase, and increasing the amount of fermentation broth used. question

Active Publication Date: 2017-03-01
SICHUAN GUANGYA POLYMER CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the storage process of the fermentation broth containing nitrile hydratase cells, when the storage time is more than or equal to 240 hours, the activity of nitrile hydratase in the fermentation broth decreases, the amount of fermentation broth increases, and the quality of the acrylamide product obtained by catalyzing the hydration reaction decreases.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] a. At 8m 3 The nitrile hydratase fermented broth ultrafiltration circulating tank was added with 4 m of fermented broth with storage time ≥ 240 hours 3 , after being ultrafiltered by an ultrafiltration membrane, put it into an ultrafiltrate storage tank, and store it at 4±1°C as a backup biocatalyst for catalytic hydration.

[0008] b. At 25m 3 In the catalytic reactor, add 17 m 3 of water, add 1.2m 3 For the nitrile hydratase fermentation broth, start to feed acrylonitrile into the catalytic reaction kettle from the high-position metering tank.

[0009] c. Control the catalytic reaction temperature between 20°C and 28°C, and control the content of acrylonitrile to <0.1% (check once every half hour).

[0010] d. Final reaction solution: acrylamide content 32%~34%, acrylonitrile content ≤0.005%, as the reaction end point.

[0011] e. Product quality indicators are shown in Table 1 below.

Embodiment 2

[0013] a. At 8m 3 The nitrile hydratase fermented liquid ultrafiltration circulating tank adds the fermented liquid 4 m of storage time 50 hours 3 , after being ultrafiltered by an ultrafiltration membrane, put it into an ultrafiltrate storage tank, and store it at 4±1°C as a backup biocatalyst for catalytic hydration.

[0014] b. At 25m 3 In the catalytic reactor, add 17 m 3 water, add 0.3m 3 For the nitrile hydratase fermentation broth, start to feed acrylonitrile into the catalytic reaction kettle from the high-position metering tank.

[0015] c. Control the catalytic reaction temperature between 20°C and 28°C, and control the content of acrylonitrile to <0.1% (check once every half hour).

[0016] d. Final reaction solution: acrylamide content 32%~34%, acrylonitrile content ≤0.005%, as the reaction end point.

[0017] e. Product quality indicators are shown in Table 1 below.

Embodiment 3

[0019] a. At 8m 3 The nitrile hydratase fermented liquid ultrafiltration circulating tank adds the fermented liquid 4 m of storage time 120 hours 3 , after being ultrafiltered by an ultrafiltration membrane, put it into an ultrafiltrate storage tank, and store it at 4±1°C as a backup biocatalyst for catalytic hydration.

[0020] b. At 25m 3 In the catalytic reactor, add 17 m 3 water, add 0.5m 3 For the nitrile hydratase fermentation broth, start to feed acrylonitrile into the catalytic reaction kettle from the high-position metering tank.

[0021] c. Control the catalytic reaction temperature between 20°C and 28°C, and control the content of acrylonitrile to <0.1% (check once every half hour).

[0022] d. Final reaction solution: acrylamide content 32%~34%, acrylonitrile content ≤0.005%, as the reaction end point.

[0023] e. Product quality indicators are shown in Table 1 below.

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Abstract

The invention provides a method for reducing a nitrile hydratase usage amount in a process of producing acrylamide by virtue of a microbiological method. In a process of producing the acrylamide by catalyzing a hydration reaction in the microbiological method, the quality of the acrylamide is optimized when a fermentation broth storage time of nitrile hydratase cells is 96-240h; and when the storage time is longer than or equal to 240h, the activity of the nitrile hydratase in a fermentation broth drops, the usage amount of the fermentation broth is increased and the quality of the obtained acrylamide is reduced. According to the method provided by the invention, by mixing the nitrile hydratase cell fermentation broth with the storage time shorter than or equal to 24h and the nitrile hydratase cell fermentation broth with the storage time longer than or equal to 240h, the activity and the quality of the nitrile hydratase are improved, so that the method for reducing the usage amount of the nitrile hydratase is achieved. With the application of the method provided by the invention, the usage amount of the nitrile hydratase in the production process is reduced, the consumption of raw materials of a fermentation medium is relieved and the stability of the quality of an acrylamide solution is improved, and the method is conducive to the subsequent production of the acrylamide. When the method provided by the invention is applied to the industrial production of the acrylamide by virtue of the microbiological method, the finished product is stable in various technical indicators.

Description

technical field [0001] In the process of producing acrylamide by microbial method, the storage time of the fermentation liquid of nitrile hydratase cells is 96-240 hours, and the quality of the product acrylamide solution obtained by the process of catalyzing hydration reaction to produce acrylamide by microbial method is the best. However, during the storage of the fermentation broth containing nitrile hydratase cells, when the storage time is more than or equal to 240 hours, the activity of nitrile hydratase in the fermentation broth decreases, the usage of fermentation broth increases, and the quality of the acrylamide product obtained by catalyzing the hydration reaction decreases. Background technique [0002] In the process of producing acrylamide by microbial method, the storage time of the fermentation liquid of nitrile hydratase cells is 96-240 hours, and the quality of the product acrylamide solution obtained by the process of catalyzing hydration reaction to produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02
Inventor 常鹏常惠联杨大海杨明树郭拥军鞠洪清吕国栋程贺
Owner SICHUAN GUANGYA POLYMER CHEM
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