Culture method of photosynthetic microorganisms and photobioreactor

Abstract

The invention relates to a culture method of photosynthetic microorganisms. According to the method, photosynthetic microorganisms are inoculated into a thin-layer liquid medium to be cultured, wherein thickness of the culture solution is not more than 10 cm; any homogenization operation is not carried out on the culture solution within the culture period; and a light source is provided for irradiation of the culture solution. A photobioreactor for culturing the photosynthetic microorganisms according to the above method is also included. The photobioreactor comprises at least a board body, which extends along the horizontal direction. The board body has opposite upper and lower surfaces, and the periphery is provided with a cofferdam part which extends upwards from the upper surface of the corresponding board body. The upper surface of each board body and the inner surface of the corresponding cofferdam part form a space for containing the culture solution for culture of the target photosynthetic microorganisms. The minimum vertical height of the cofferdam part is not more than 10 cm. The invention also relates to a photobioreactor containing multilayer of the board bodies which are stacked at certain interval in the vertical direction.

Description

technical field [0001] The invention relates to the cultivation field of photosynthetic microorganisms, in particular to a cultivation method in a static thin-layer liquid cultivation mode and a photobioreactor. Background technique [0002] Photosynthetic microorganisms are a group of microorganisms that use light as their sole or main energy source, including microalgae, cyanobacteria and other microorganisms that contain chlorophyll and can perform photosynthesis. This kind of microorganism has important biological utilization value, especially microalgae, which is rich in protein and can be used as aquatic bait or livestock feed (such as spirulina); more importantly, some microalgae can synthesize a large amount of secondary metabolism under certain conditions substances, such as oils, carotenoids, polysaccharides, etc. These substances are often biologically active substances with extremely high economic value, and can be used in functional foods, food additives, pharma...

AI Technical Summary

Problems solved by technology

However, this solid-state culture method often depends to a large extent on the water retention performance of porous materials and the adhesion characteristics of algae. If the water retention of the material is poor, it needs to supply water continuously, which consumes energy; If the species is easy to detach, it will not be able to make the algae grow normally

These porous materials are often opaque materials, which will absorb more light energy. The efficiency of light utilization by algae species is not high. Difficulty with disinfection

Moreover, this solid-state culture method is often very unsuitable for the cultivation of larger algae, such as Nostocs, Gexianmi and Dipis

[0009] To sum up, the existing photobioreactors generally have the following technical problems: ①, the open raceway pool occupies a large area, the culture density is low, the energy consumption of the culture medium circulation is high, and it is easy to be polluted by the outside world; ②, the closed type The cost of light-transmitting containers is high, especially glass containers, which are limited by the particularity of glass processing technology and cannot be molded at one time. The manufacturing cost, installation and maintenance costs are very high, and there are also low cell density, insufficient space utilization, and high energy consumption. The problem

③. Whether it is an open track pool or a closed light-transmitting container, it needs to consume a lot of culture fluid and energy. The concentration of the culture fluid is low when harvesting, and it needs to go through cumbersome separation, filtration and drying procedures. Technical defects cannot reduce the microalgae Product Cost

④The solid-state culture photobioreactor is very dependent on the material itself, the light utilization efficiency is low, and its applicability is relatively limited. The liquid supply device is an energy-consuming device, so it cannot further reduce the culture cost

⑤ The existing photobioreactors are not conducive to the effective isolation and control of pollution sources

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