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Method for preparing antibacterial biological pesticide by using psidium guajava linn leaf extract

A technology of guava leaves and biopesticides, applied in botany equipment and methods, chemicals for biological control, biocides, etc., can solve the problems that the application of guava leaves has not been reported.

Inactive Publication Date: 2017-03-01
SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The application of guava leaves in agriculture as botanical pesticides has not been reported

Method used

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  • Method for preparing antibacterial biological pesticide by using psidium guajava linn leaf extract
  • Method for preparing antibacterial biological pesticide by using psidium guajava linn leaf extract
  • Method for preparing antibacterial biological pesticide by using psidium guajava linn leaf extract

Examples

Experimental program
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Effect test

preparation example 1

[0040] A. Primary processing of guava leaves

[0041] (1) Drying: the mature guava leaves collected are dried to a moisture content of ≤10% by using the sunlight drying method;

[0042] (2) impurity removal: process and separate the dried guava leaves through a vibrating sorting sieve, and filter out impurities;

[0043] (3) pulverizing: pulverizing the qualified guava leaf processed through the above process, pulverizing through 80 mesh sieves;

[0044] B, the preparation of the extract of guava leaf

[0045] (1) Rough extraction: heat the guava leaf powder after primary processing at 80° C., use 80% ethanol as the extractant, and have a solid-liquid ratio of 20 times, and extract under reflux for 3 hours. Prepare the extract of guava leaf flavonoids;

[0046] (2) Fine extraction: Repeatedly extract 2 times by the above extraction process, until basically the active ingredients in the guava leaf powder are extracted;

[0047] C, the concentration of guava leaf extract

...

preparation example 2

[0058] A. Primary processing of guava leaves

[0059] (1) Drying: take the collected mature guava leaves and dry them in the sun to contain moisture≤10%;

[0060] (2) impurity removal: process and separate the dried guava leaves through a vibrating sorting sieve, and filter out impurities;

[0061] (3) pulverizing: pulverizing the qualified guava leaf processed through the above process, pulverizing through a 100 mesh sieve;

[0062] B, the preparation of the extract of guava leaf

[0063] (1) Rough extraction: heat the guava leaf powder after primary processing at 75° C., use 70% ethanol as the extractant, and have a solid-liquid ratio of 25 times, and extract under reflux for 4 hours. Prepare the extract of guava leaf flavonoids;

[0064] (2) Fine extraction: Repeatedly extract 2 times by the above extraction process, until basically the active ingredients in the guava leaf powder are extracted;

[0065] C, the concentration of guava leaf extract

[0066] (1) Centrifuga...

Embodiment 3

[0077] A. Primary processing of guava leaves

[0078] (1) Drying: take the collected mature guava leaves and dry them in the sun to contain moisture≤10%;

[0079] (2) impurity removal: process and separate the dried guava leaves through a vibrating sorting sieve, and filter out impurities;

[0080] 3) pulverizing: pulverize the qualified guava leaves processed through the above process, and pulverize through an 80 mesh sieve;

[0081] B, the preparation of the extract of guava leaf

[0082] (1) Rough extraction: heat the guava leaf powder after primary processing at 70° C., use 60% ethanol as the extractant, and have a solid-liquid ratio of 15 times, and extract under reflux for 2 hours. Prepare the extract of guava leaf flavonoids;

[0083] (2) Essence extraction: extract repeatedly 3 times by above extraction process, until basically extracting the active ingredient in the guava leaf powder;

[0084] C, the concentration of guava leaf extract

[0085] (1) Centrifugation...

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Abstract

The invention discloses a method for preparing an antibacterial biological pesticide by using a psidium guajava linn leaf extract. The method comprises: A, primary processing of psidium guajava linn leaf; B, preparation of a psidium guajava linn leaf extract; C, concentration of a psidium guajava linn leaf extraction liquid; D, degreasing; E, drying and grinding; and F, preparation of the pesticide from the dry powder. According to the present invention, the dry powder can be prepared into the aqueous solution with the concentration of 15-150 mg / ml so as to be used for prevention and control of bacteria in plants, especially vegetables.

Description

technical field [0001] The invention relates to a preparation method in the technical field of biological pesticides, in particular to a method for preparing antibacterial biological pesticides by using guava leaf extracts. Background technique [0002] Guava (Psidium guafaVB L.) commonly known as plucked fruit, chicken arrow fruit, guava, red heart fruit, trumpet fruit, etc., is a plant of the genus Guava in the family Myrtaceae, and is an evergreen small tree or shrub. Native to tropical America and Southeast Asia, it is now widely distributed and planted in tropical and subtropical regions. Guava is one of the fruits with rapid development and good economic benefits in Xiamen in recent years. [0003] The fresh leaves of guava and the dried leaves with leafy tender stems are called "Maguo" in the Brown language. Guava leaves are rich in flavonoids, phenolic compounds, polysaccharides, inorganic elements, volatile oils, saponins, oleanolic acid and ursolic acid and other...

Claims

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Application Information

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IPC IPC(8): A01N65/28A01P3/00
Inventor 陈淳林清洪刘黎卿陈菲陈丽璇林志楷刘鸿洲
Owner SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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