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Co-enrichment medium SSEL

A technology of enrichment medium and basic medium, which is applied in the field of agricultural product testing, to achieve the effect of testing a wide range of samples, avoiding the interference of miscellaneous bacteria, and saving quantity

Pending Publication Date: 2017-02-22
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the co-enrichment culture of Salmonella, Staphylococcus aureus, Escherichia coli O157:H7 and Listeria monocytogenes (marked as SSEL in this patent) that are common in edible agricultural products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Verification of the separate enrichment effect of the co-enrichment culture medium SSEL

[0041] Preparation of SSEL medium: Weigh 30 parts of tryptone soybean broth, 6 parts of yeast extract, 10 parts of sodium deoxycholate, and 15 parts of lithium chloride, add 1000 parts of deionized water, stir well, and heat at 121 ° C under high pressure Sterilize and cool to room temperature. Then, aseptically add 0.012 parts of acriflavine and 0.0025 parts of nalidixic acid, and stir evenly to prepare SSEL medium.

[0042] SSEL enrichment culture: 10 1 one 10 2 CFU / mL of Salmonella, Staphylococcus aureus, Escherichia coli O157:H7, and Listeria monocytogenes were inoculated into 100mL SSEL and their respective selective enrichment solutions (Salmonella: RV, Staphylococcus aureus: 7.5% sodium chloride In broth, Escherichia coli O157:H7:mEC+n, Listeria monocytogenes:FB), shake culture at 37°C for 24h, take the culture once every 4h, and use the selective isolation med...

Embodiment 2

[0043] Example 2: Verification of SSEL compound bacterial enrichment effect

[0044] Preparation of SSEL medium: refer to Example 1.

[0045] SSEL enrichment culture: Salmonella, Staphylococcus aureus, Escherichia coli O157:H7, and Listeria monocytogenes were respectively divided into 1:1:1:1 (10 2 CFU / mL), 1:10:100:1000 (10 1 one 10 4 CFU / mL), 10:1:1000:100, 100:1000:1:10, 1000:100:10:1 were mixed and inoculated into SSEL, cultured with shaking at 200rpm at 37°C for 24h, and the culture was taken every 4h , counted with the selective isolation medium of each target bacteria ( figure 2 ). from figure 2 It can be seen that when the inoculation amount of the four target bacteria is the same, Salmonella, Staphylococcus aureus, O157:H7 and Listeria monocytogenes grow well in SSEL, and the growth rate, lag period and stable period are relatively similar. When the inoculation amount of Gram-positive bacteria is low (SA:LM:O157:SE=1:10:100:1000, SA:LM:O157:SE=10:1:1000:100), ...

Embodiment 3

[0046] Example 3: Verification of SSEL's ability to repair cold-injured target bacteria

[0047] Preparation of SSEL medium: refer to Example 1.

[0048] Treatment of samples to be tested: Take 10g of commercially available raw pork and lettuce, soak them in alcohol, disinfect and air-dry them, and then inoculate them with Salmonella enteritidis (ATCC 13076), Staphylococcus aureus (ATCC 25913), O157:H7 from the large intestine, and Les cereus monocytogenes. Special bacteria (F 2365), the final concentration is about 10CFU / g, air-dried until the bacteria solution is completely absorbed. Lettuce samples were stored in a refrigerator at 4°C for 2 days. Pork samples were stored in a -20°C refrigerator for 10.5 days.

[0049] SSEL enrichment culture: put 10 g of the above artificially polluted pork and lettuce samples into 90 ml SSEL, and culture at 36° C. for 24 hours. Take a culture every 4h, and count with the selective isolation medium of each target bacteria ( image 3 ). ...

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Abstract

The invention provides a co-enrichment medium SSEL. The co-enrichment medium SSEL is prepared from components as follows: a basic medium, an inhibitor and water. The co-enrichment medium can enrich Salmonella enteritidis, Staphylococcus aureus, Escherichia coli O157:H7 and Listeria monocytogenes simultaneously and can be combined with other detection technologies to detect multiple pathogenic bacteria simultaneously.

Description

technical field [0001] The invention relates to the field of agricultural product detection, in particular to a co-enrichment culture medium SSEL capable of simultaneously enriching Salmonella enteritidis, Staphylococcus aureus, Escherichia coli O157:H7 and Listeria monocytogenes. Background technique [0002] Salmonella, Staphylococcus aureus, Escherichia coli and Listeria monocytogenes are common food-borne pathogens, and they are detected in edible agricultural products to a certain extent It is the main pathogenic bacteria that contaminates this kind of food, and has caused many large-scale food poisoning incidents. The development of rapid detection methods for foodborne pathogens is of great significance for the timely and effective control of the spread of foodborne outbreaks and the prevention of similar incidents. [0003] To detect food-borne pathogens, the first step is to select a suitable enrichment solution to enrich the sample for 16-24 hours. This process ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/14C12Q1/10C12Q1/04
CPCC12Q1/04C12Q1/10C12Q1/14Y02A50/30
Inventor 索玉娟周昌艳瞿洋高士刚沈源源
Owner SHANGHAI ACAD OF AGRI SCI
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