Metabolites of Rieteria in Grass Carp
A metabolite, Rieter mold technology, applied in the field of aquaculture, can solve problems that have not been reported
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Embodiment 1
[0027] 1 Materials and methods
[0028] 1.1 Test time and place
[0029] From March 2010 to August 2010, Shanghai Ocean University, No. 999 Huchenghuan Road, Lingang New City, Pudong New Area, Shanghai.
[0030] 1.2 Test drugs, test fish and breeding conditions
[0031] The Rieter mold raw material used in the test was provided by Changsha Better Biotechnology Research Institute Co., Ltd.
[0032] Rieter mold reference substance: purchased from Beijing Century Aoke Biotechnology Co., Ltd., Dr.Ehrenstorfer, Germany, CAS number 57837-19-1 (Water Content0.1%, Det.Purity99.0%, Tolerance / Uncertainty+ / -0.5% , Determined by Karl-Fischer Titration).
[0033] Healthy grass carp were purchased from Sunnong Aquaculture Farm in Pudong, Shanghai, 1000 each, weighing (250±10) g, placed in an aquarium (100cm×80cm×50cm) sterilized by potassium permanganate for 5 days , the experimental water is fully aerated tap water, and the air pump continuously aerates the fish to remove dead and unhe...
Embodiment 2
[0050] 2.1.1 HPLC-MS / MS analysis of prototype M0
[0051] The chromatographic retention time of M0 is 9.6min, and its chromatographic behavior, mass-to-charge ratio of quasi-molecular ions and fragment ions are all consistent with the Rieter mold reference substance, so it is determined that M0 is the original form of Rieter mold.
[0052] 2.1.2 HPLC-MS / MS analysis of metabolite M3
[0053] The chromatographic retention time of M3 was 8.4min, and its quasi-molecular ion m / z252.1230 was observed in the blood sample primary positive ion full-scan mass spectrometry. In the further fragmentation mode of MS / MS, it was observed that the fragment ion m / z252.1220 was 28 less than the quasi-molecular ion of Rietermycin, which was speculated to be the bis-demethylation product of Rietermycin. Therefore, it is speculated that M3 is the double demethylation metabolite of Rieter, which is N-(2-carboxy-6-methylphenyl)-N-(methoxyacetyl)alanine, and the Chinese name is N-(2-carboxy-6-benzyl)...
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