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Metabolites of Ridomil in ctenopharyngodon idellus

A technology of Rieter mold and metabolites, applied in the field of aquaculture, can solve problems such as unreported

Inactive Publication Date: 2017-02-22
SHANGHAI OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] There are many reports about Rieter mold residues in the patent literature. For example, Chinese patent literature CN104535671A discloses a detection method for metalaxyl residues in eggplant, and Chinese patent literature CN105259351A discloses a test paper for detecting metalaxyl. Document CN104391068A discloses a method for measuring the residual amount of commonly used bactericides (such as metalaxyl, etc.) in tobacco, and Chinese patent document CN1959406A discloses a method for detecting residuals of metalaxyl, etc. in vegetables, but regarding the standpoint of the present invention The metabolites of Daxamicin in grass carp have not been reported yet

Method used

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  • Metabolites of Ridomil in ctenopharyngodon idellus

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Effect test

Embodiment 1

[0027] 1 Materials and methods

[0028] 1.1 Test time and place

[0029] From March 2010 to August 2010, Shanghai Ocean University, No. 999 Huchenghuan Road, Lingang New City, Pudong New Area, Shanghai.

[0030] 1.2 Test drugs, test fish and breeding conditions

[0031] The Rieter mold raw material used in the test was provided by Changsha Better Biotechnology Research Institute Co., Ltd.

[0032] Rieter mold reference substance: purchased from Beijing Century Aoke Biotechnology Co., Ltd., Dr.Ehrenstorfer, Germany, CAS number 57837-19-1 (Water Content0.1%, Det.Purity99.0%, Tolerance / Uncertainty+ / -0.5% , Determined by Karl-Fischer Titration).

[0033] Healthy grass carp were purchased from Sunnong Aquaculture Farm in Pudong, Shanghai, 1000 each, weighing (250±10) g, placed in an aquarium (100cm×80cm×50cm) sterilized by potassium permanganate for 5 days , the experimental water is fully aerated tap water, and the air pump continuously aerates the fish to remove dead and unhe...

Embodiment 2

[0050] 2.1.1 HPLC-MS / MS analysis of prototype M0

[0051] The chromatographic retention time of M0 is 9.6min, and its chromatographic behavior, mass-to-charge ratio of quasi-molecular ions and fragment ions are all consistent with the Rieter mold reference substance, so it is determined that M0 is the original form of Rieter mold.

[0052] 2.1.2 HPLC-MS / MS analysis of metabolite M3

[0053] The chromatographic retention time of M3 was 8.4min, and its quasi-molecular ion m / z252.1230 was observed in the blood sample primary positive ion full-scan mass spectrometry. In the further fragmentation mode of MS / MS, it was observed that the fragment ion m / z252.1220 was 28 less than the quasi-molecular ion of Rietermycin, which was speculated to be the bis-demethylation product of Rietermycin. Therefore, it is speculated that M3 is the double demethylation metabolite of Rieter, which is N-(2-carboxy-6-methylphenyl)-N-(methoxyacetyl)alanine, and the Chinese name is N-(2-carboxy-6-benzyl)...

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Abstract

The invention relates to metabolites of Ridomil in ctenopharyngodon idellus. The metabolites of Ridomil in ctenopharyngodon idellus can be N-(2-carboxyl-6-benzyl)-N-(methoxyacetyl)alanine, N-(2-hydroxymethyl)-6-tolyl-N-(glycolyl)alanine, N-(2, 6-xylyl)-N-(glycolyl)alanine or N-(2-hydroxymethyl-6-benzyl)-N-(methoxyacetyl)alanine methyl ester. After dosing ctenopharyngodon idellus with Ridomil, the invention for the first time finds that in a mouth filling group and a soaking group, Ridomil in liver tissue is based on prototype drug, and finds the metabolites of the aforementioned four Ridomil in ctenopharyngodon idellus.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to the metabolite of Rieter mold in grass carp. Background technique [0002] Grass carp (Ctenopharyngodon idellus) belongs to the genus Grass Carp of Cyprinidae Cyprinidae, and it inhabits rivers and lakes in plain areas. It is lively, swims fast, and often feeds in groups. It is a typical herbivorous fish. In the juvenile stage, grass carp eats larvae, algae, etc. Grass carp also eats some meat, such as earthworms, dragonflies, etc., and overwinters in the deep water of main streams or lakes. Broodstock have the habit of swimming in the breeding season. Because of its rapid growth and wide source of feed, it is one of the four major domestic fish in freshwater farming in China, and it is the main freshwater fish breeding target in China. [0003] Saprolegnia is a common fungal species in freshwater aquaculture water, infecting almost all freshwater fish. Malachite green is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C235/68G01N30/02
CPCC07C235/68G01N30/02G01N2030/027
Inventor 吕新美董亚萍胡鲲孙晶姜莺颖张一柳刘力硕程俊茗朱凤娇吴映捷董龙香范宁宁
Owner SHANGHAI OCEAN UNIV
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