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Sunflower straw bacteriostatic agent, preparation method and application in preventing and treating potato early blight

The technology of sunflower and bacteriostatic agent is applied in the field of new pesticides and preparation, which can solve the problems of unsatisfactory control effect of chemical agents and high cost of use, and achieve the effects of easy acquisition, reduced processing cost and wide source.

Inactive Publication Date: 2018-10-23
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In actual production, there are few potato varieties resistant to early blight, and they mainly rely on chemical agents for control. The commonly used fungicides mainly include mancozeb, chlorothalonil, azoxystrobin, etc., but the control effects of these chemical agents are not ideal, and the cost of use Higher, and foreign reports have produced resistance to Alternastrobin
In order to improve the control effect and reduce the cost, in the prior art, fungicides in production are often mixed to delay the emergence of pathogenic bacteria resistance. For example, Tai Lianmei et al. Effects on Yield (Efficacy of mixed fungicides on potato early blight and its impact on yield, Tailianmei, Jiangsu Agricultural Sciences, Vol. 42, No. 9, 2014: 110-112), using azoxystrobin and Dysen The mixed use of manganese and zinc achieves complementary advantages, but the mixed fungicides can still promote the resistance of bacteria

Method used

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  • Sunflower straw bacteriostatic agent, preparation method and application in preventing and treating potato early blight
  • Sunflower straw bacteriostatic agent, preparation method and application in preventing and treating potato early blight

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preparation example Construction

[0045] The present invention also provides a kind of preparation method of described sunflower stalk antibacterial agent, comprises the following steps:

[0046] 1) Pulverizing the sunflower stalks to obtain sunflower stalk powder;

[0047] 2) mixing the sunflower stalk powder with a solvent and extracting to obtain an extract, the solvent being petroleum ether, ethyl acetate or petroleum ether;

[0048] 3) centrifuging the extract obtained in step 2), and the obtained supernatant is sunflower stalk antibacterial agent.

[0049] In the present invention, the sunflower stalk powder is passed through a sieve of 60-100 mesh, more preferably 70-90 mesh, before being mixed with the solvent.

[0050] In the present invention, the rotational speed of the centrifugation is preferably 2000-4000 r / min, more preferably 3500 r / min; the centrifugation time is preferably 10-20 min, more preferably 15 min.

[0051] In the present invention, the obtained sunflower stalk antibacterial agent ...

Embodiment 1

[0059] The sunflower stalks free from insect damage and mildew are selected as raw materials, processed into powder by a pulverizer, passed through a 95-mesh sieve, and the obtained sunflower stalk powder is collected. Weigh 1 kg of the powder, mix it with 10 L of petroleum ether, put it into a barrel and extract it for 3 days, centrifuge the extract solution at a speed of 3000 r / min for 20 min, collect the supernatant, and concentrate it by rotary evaporation at 45 ° C for 2 h. Vacuum freeze-drying to obtain extract powder, which is stored in a refrigerator at 4°C for future use.

Embodiment 2

[0061] The sunflower stalks free from insect damage and mildew are selected as raw materials, processed into powder by a pulverizer, passed through a 70-mesh sieve, and the obtained sunflower stalk powder is collected. Weigh 1 kg of the powder, mix it with 15 L of petroleum ether, put it into a barrel for extraction for 5 days, centrifuge the extraction solution at a speed of 4000 r / min for 10 min, collect the supernatant, and concentrate it by rotary evaporation at 35 ° C for 3 h. Vacuum freeze-drying to obtain extract powder, which is stored in a refrigerator at 4°C for future use.

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Abstract

The invention provides a sunflower straw bacteriostatic agent. The sunflower straw bacteriostatic agent comprises a sunflower straw extract obtained via extraction from sunflower straw with a solvent, and the solvent is petroleum ether. A preparation method comprises following steps: 1, sunflower straw is smashed so as to obtain sunflower straw powder; 2, the sunflower powder is mixed with the solvent for extraction so as to obtain an extracted liquid; and 3, the extracted liquid is subjected to centrifugalization, and an obtained supernate is the sunflower straw bacteriostatic agent. In applications of the sunflower straw bacteriostatic agent in preventing and treating alternaria solani sorauer, bacteriostasis rate achieves 10.57% to 18.02% when mass concentration of the sunflower straw bacteriostatic agent is controlled to be 80 to 360mg / ml.

Description

technical field [0001] The invention relates to a novel pesticide, its preparation method and application, in particular to a sunflower stalk antibacterial agent, its preparation method and its application in preventing and treating potato early blight. Background technique [0002] Potato early blight (Alternaria solani Sorauer) is a worldwide disease caused by Alternaria alternata, which is widely distributed in potato production areas in my country. Early blight mostly occurs in the later stage of potato growth, causing the leaves to dry out in advance, reducing yield, and some severe plots have no harvest in the whole field. In recent years, early blight has frequently regenerated in many potato producing areas and has an upward trend. It can cause a 5% to 10% reduction in potato production in a normal year, and the production reduction can reach more than 50% in a severe year. Storage tuber rot caused by Alternaria alternata has also become a serious problem in the pot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/12A01P3/00
CPCA01N65/00A01N65/12A01N2300/00
Inventor 赵萍肖宇轩王聪夏文旭雷晨瑶邱悦芮文君
Owner LANZHOU UNIVERSITY OF TECHNOLOGY
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