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Bacillus with high temperature resistance and cellulose degradation capacity and application thereof

A technology of cellulose degradation and bacillus, applied in the fields of application, bacteria, organic fertilizers, etc., can solve the problems of pectin and hemicellulose component decomposition, high temperature tolerance and other problems, achieve low cost of cultivation, rapid reproduction, The effect of facilitating the implementation of industrialization

Active Publication Date: 2017-01-25
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defects that the existing applications involving Bacillus cannot decompose the pectin and hemicellulose components in the straw, and have limited high-temperature tolerance, the present invention proposes a high-temperature-resistant Bacillus and cellulose-degrading ability. In its application, the bacterium not only has a strong degradative ability to cellulose, but also has a certain degradative ability to hemicellulose, pectin and lignin components in the straw; at the same time, the bacterium can grow and reproduce at a high temperature of 60°C , and can withstand the high temperature of 100 ℃, has a good application prospect of high temperature composting

Method used

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  • Bacillus with high temperature resistance and cellulose degradation capacity and application thereof
  • Bacillus with high temperature resistance and cellulose degradation capacity and application thereof
  • Bacillus with high temperature resistance and cellulose degradation capacity and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] 1. Isolation, purification and preservation of strains

[0026] The rotten rice straw and soil were taken from the Shanghai Jiaotong University Pujiang Green Valley Experimental Base in Pujiang Town, Minhang District, Shanghai as samples. 10 g of different samples were ground and placed in 100 mL enrichment medium for constant temperature cultivation at 37 °C and 150 rpm. Every 5 days, 10% of the inoculum was added to another enriched medium, and the acclimatization was repeated 4 times. The enrichment medium adopts the medium with CMC-Na as the sole carbon source.

[0027] Take 100 μL of the enrichment solution and spread it on the CMC-Congo red medium plate, and then culture it upside down in a 35°C incubator at a constant temperature. Observe after 5 days that a transparent hydrolysis circle appears on the plate. Pick a small amount of bacteria and streak on LB solid medium until a single colony is obtained, and then continuously culture the obtained single colony o...

Embodiment 2

[0040] Preparation of High Temperature Composting Bacteria

[0041] The bacteria were inoculated in LB liquid medium, and after 24 hours, samples were taken to determine the concentration of the bacteria solution was about 2.0×10 10 After cfu / mL, stop the fermentation. Mix each liter of bacterial liquid with 0.4-0.6 kg of rice straw to prepare a high-temperature compost bacterial agent.

Embodiment 3

[0043] Application of High Temperature Composting Bacteria in Rice Straw Composting

[0044]The rice straw is crushed to 3-5cm by crushing equipment to form a straw segment, and 1.0% of the weight of the straw is added to the compost bacterial agent, and the water content of the pile is adjusted to about 65%. Measure the temperature of the heap body every day. At the beginning, the temperature of the heap body will rise rapidly to around 70°C and maintain for 1-2 days. When the temperature reaches the peak and gradually drops below 45°C, it means that the fermentation is completed (such as Figure 7 ). The whole composting process is about 7-8 days and the compost is turned once, and the compost is turned 3 times in total. After the third turning is completed, the straw bio-organic fertilizer is obtained.

[0045] After searching the prior art, it is found that Chinese Patent Document No. CN103013887, published on 2013-04-03, discloses a Bacillus pumilus KMXU56, and its pres...

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Abstract

The invention relates to a bacillus with high temperature resistance and cellulose degradation capacity and an application thereof. The invention specifically relates to bacillus JWT-1, belongs to Bacillus subtilis and is preserved in China General Microbiological Culture Collection Center (CGMCC); the preserving date is on July 11, 2016; the preserving No. is CGMCC No.12758. The bacillus has higher degradation capacity for cellulose, hemicellulose, pectin and lignin in straws; the bacillus can grow and multiply under high temperature of 60 DEG C; the highest endurable temperature is 100 DEG C; the bacillus has excellent high-temperature compost application prospect.

Description

technical field [0001] The invention relates to a technology in the field of biological environmental protection, in particular to a high-temperature-resistant bacillus with cellulose-degrading ability and an application thereof. Background technique [0002] Cellulose is the main product of plant photosynthesis and is the most abundant renewable resource in nature. The annual output of crop straw in the world is about 3 billion tons, and the annual output of straw in my country alone exceeds 700 million tons. The cellulose content in straw is about 40%. Cellulose is a high-molecular polymer containing a large number of high-energy hydrogen bonds, which cannot be directly absorbed and utilized by crops. Straw returning directly to the field will affect the growth of crops, and straw burning will cause air pollution. According to statistics, nearly 90% of the straw is discarded or burned without being effectively utilized and polluting the environment. [0003] In today's s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C05F15/00C12R1/125
CPCC05F11/00C05F11/08C12N1/205C12R2001/125C05F17/00
Inventor 周培冯海玮支月娥聂文翰张丹詹学佳冯洁初少华
Owner SHANGHAI JIAO TONG UNIV
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