Separation/purification and pathogenicity identification method for pathogenic bacteria of fusarium root rot of Medicago sativa

Abstract

The invention discloses a separation / purification and pathogenicity identification method for pathogenic bacteria of fusarium root rot of Medicago sativa. The method comprises the specific steps of collecting and preparing diseased Medicago sativa plants, carrying out separation and purification on the pathogenic bacteria, carrying out morphological observation on the pathogenic bacteria, carrying out molecular biology identification, constructing an evolutionary tree and carrying out pathogenicity identification. According to the method, the identification on pathogens of root rot of the Medicago sativa is simple in operation and high in accuracy and is simple, convenient and rapid; the separation and purification purity is high, the identification result is reliable, and the strength of pathogenicity of each strain is determined through pathogenicity identification.

Description

technical field [0001] The invention relates to the field of analysis of plant pathogenic bacteria, in particular to a method for separation, purification and pathogenicity identification of alfalfa pathogenic bacteria. Background technique [0002] Alfalfa (Medicago sativa L.) is the most widely distributed perennial leguminous forage in the world. It has the reputation of "the king of forage" due to its high yield and excellent quality. Another great advantage of alfalfa is that it can be used for many years once planted. Due to the long use period, root rot has become an extremely important reason for yield decline and plant decline. The disease is a worldwide disease and occurs in almost all alfalfa producing areas. It is estimated that the annual yield loss caused by the disease is about 20% in the world, and some serious occurrences even reach 40%. With the increase of the planting area and the extension of the planting period, the disease problem will become more and...

AI Technical Summary

Problems solved by technology

[0004] In the past, the research on alfalfa root rot in my country has been focused on field symptoms, control, etc., and there are not many reports on the research on the pathogenic system.

In addition, Fusarium is one of the most difficult to identify and most economically valuable genus in fungi. It has complex morphology and is easily mutated by the external environment. It is difficult to accurately identify the species through morphological observation.

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