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Method of identifying foreign colloid-agar in shrimp body

A shrimp and colloid technology, which is used in material analysis, measuring devices, instruments and other directions by observing the impact on chemical indicators, which can solve the problems of no clear detection items and detection purposes, and difficult qualitative detection and identification of "colloidal substances". , to achieve the effect of easy promotion and application, simple identification and operation

Inactive Publication Date: 2017-09-29
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the "colloidal substance" in the "glue-injected" shrimp is an unknown sample, which is a "blind sample" for the testing agency, without clear testing items and testing purposes, so it is difficult for the testing agency to conduct qualitative testing of the "colloidal substance". identification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The method for identifying the foreign colloid-agar in the shrimp body provided by the present embodiment comprises the following steps:

[0035] 1. Test method:

[0036] (1) Thaw the frozen shrimp sold in the market first, then peel off the shell to remove the muscle, collect all the shrimp body tissues other than the muscle such as the shrimp head, shrimp shell and thymus body fluid; take 10g of the shrimp body tissue and put it in a beaker, add 100mL of distilled water, and stir Boil for 5 minutes, then filter while hot with a 200-mesh filter cloth to collect the filtrate; centrifuge the filtrate at 4500r / m for 10 minutes, filter the supernatant through medium-speed filter paper to collect the filtrate; repeat the centrifugation and filtration of the filtrate once more, and collect the filtrate in a beaker; slowly heat the beaker to evaporate part of the water until the remaining solution in the beaker is about 10mL;

[0037] (2) Cool the solution in the beaker of s...

Embodiment 2

[0048] The method for identifying the foreign colloid-agar in the shrimp body provided by the present embodiment comprises the following steps:

[0049] 1. Test method:

[0050] (1) Select the chilled fresh shrimp samples sold in the market, peel off the shrimp muscle tissue, collect all the shrimp body tissues except the muscle such as the shrimp head, shrimp shell and thymus body fluid; take about 15g of the shrimp body tissue and put it in a beaker, add distilled water 150mL, stir and boil for about 10min, filter while hot with 240 mesh filter cloth, and collect the filtrate; centrifuge the filtrate at 10000r / m for 5min, filter the supernatant through medium-speed filter paper, collect the filtrate; repeat the centrifugation and filtration once again for the filtrate , collect the filtrate in a beaker; heat the beaker slowly to evaporate part of the water until the remaining solution in the beaker is about 15mL;

[0051] (2) naturally cool the solution in the beaker of ste...

Embodiment 3

[0062] The method for identifying the foreign colloid-agar in the shrimp body provided by the present embodiment comprises the following steps:

[0063] (1) Select commercially available frozen shrimp, thaw the frozen shrimp sample with running water first, peel off the shrimp muscle tissue, collect all the shrimp body tissues except the muscle such as the shrimp head, shrimp shell and thymus body fluid; take about 15g of the shrimp body tissue Put in a beaker, add 150mL of distilled water, boil for 10min under stirring, filter while hot with a 180-mesh filter cloth, and collect the filtrate; centrifuge the filtrate at 10000r / m for 5min, filter the supernatant through a medium-speed filter paper, and collect the filtrate; repeat the filtrate Centrifuge and filter once, collect the filtrate in a beaker; heat the beaker slowly to evaporate part of the water until the remaining solution in the beaker is about 15mL;

[0064] (2) Cool the solution in the beaker of step (1) to room ...

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Abstract

The invention discloses a method of identifying a foreign colloid-agar in a shrimp body. The method comprises the following steps of: firstly, selecting a shrimp body tissue to prepare a shrimp body tissue solution; cooling the shrimp body tissue solution to room temperature; observing the gel condition of the solution; judging whether the shrimp body contains agar or not according to the gel condition; then heating the gel to be molten to form a solution; then preparing a colloid; adding a NaOH solution and an alkaline cupric tartrate solution into the colloid; judging whether the colloid is a polysaccharide substance or not according to color change of the solution; meanwhile, adding an I2 solution into the colloid to observe the dyeing condition of the colloid; and judging whether the colloid is agar or not according to the color change of the colloid. The method can identify whether the shrimp body contains the foreign colloid or not and whether variety of the foreign colloid is agar or not.

Description

technical field [0001] The invention belongs to the technical field of aquatic product quality and safety, and in particular relates to a method for identifying foreign colloid-agar in shrimp bodies. Background technique [0002] As early as around 2000, chilled and frozen prawns suspected of being "glue-injected" appeared in the Chinese aquatic product market. For more than a decade, this kind of glue-injected shrimp has been used by consumers in more than ten coastal and inland cities. Or local news media exposure, causing widespread concern about food safety issues. But what exactly is the "glue-like substance" in shrimp? Is it harmful to the human body? It has become a major problem for both law enforcement and consumers. Because the testing organization needs to have a basis for the testing method standard to make a testing judgment. However, the "colloidal substance" in the "glue-injected" shrimp is an unknown sample, which is a "blind sample" for the testing agenc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/786
Inventor 杨贤庆戚勃李来好杨少玲邓建朝马海霞赵永强吴燕燕荣辉林婉玲郝淑贤胡晓
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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