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A resource utilization method of kudzu root for separation and purification of flavonoids, starch and amino acids

A technology of flavonoids and amino acids, which is applied in chemical instruments and methods, organic chemistry, cyanide reaction preparation, etc., can solve the problems of sufficient extraction of unfavorable substances, neglect of amino acid recovery, low product purity, etc., and achieves low production costs and reduced production. Cost and raw materials are cheap and easy to obtain

Active Publication Date: 2019-05-24
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problem with this method is that: only the starch and total flavonoids in Pueraria lobata are recovered, and the recovery of amino acids is ignored, resulting in a waste of resources
The problem with this method is that the treatment process is relatively simple, which is not conducive to the sufficient extraction of substances, and the purity of the isolated product is not high.

Method used

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  • A resource utilization method of kudzu root for separation and purification of flavonoids, starch and amino acids

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Example 1, see figure 1 :

[0041] The present invention provides a resource utilization method of Pueraria lobata root for separating and purifying flavonoids, starch and amino acids, which comprises the following steps:

[0042] 1) Pretreatment: After cleaning the commercially available fresh kudzu root, scrape the skin, cut into thin strips, grind and squeeze the thin kudzu root strips, add tap water while grinding and squeezing to obtain a crude kudzu root slurry;

[0043] 2) Cellulase hydrolysis: adjust the slurry temperature to 35°C, add cellulase to break the cell wall and release the starch and protein in the kudzu root. The added mass of cellulase is 2% of the fresh kudzu root mass;

[0044] 3) Ultrasonic extraction: Add ethanol to the hydrolyzed slurry, keep the volume concentration of ethanol at 60% and temperature at 60°C, perform ultrasonic extraction, with ultrasonic power of 100W, ultrasonic frequency of 40KHz, and extraction for 30 minutes. Flavonoids are relea...

Embodiment 2

[0053] The present invention provides a resource utilization method of Pueraria lobata root for separating and purifying flavonoids, starch and amino acids, which comprises the following steps:

[0054] 1) Pretreatment: After cleaning the commercially available fresh kudzu root, scrape the skin, cut into thin strips, grind and squeeze the thin kudzu root strips, add tap water while grinding and squeezing to obtain a crude kudzu root slurry;

[0055] 2) Cellulase hydrolysis: adjust the slurry temperature to 30°C, add cellulase to break the cell wall, and release the starch and protein in the kudzu root. The added mass of cellulase is 4% of the mass of fresh kudzu root;

[0056] 3) Ultrasonic extraction: Add ethanol to the hydrolyzed slurry, keep the volume concentration of ethanol at 60% and temperature at 60°C, perform ultrasonic extraction, with ultrasonic power of 100W, ultrasonic frequency of 40KHz, and extraction for 30 minutes. Flavonoids are released, and Pueraria lobata extrac...

Embodiment 3

[0065] The present invention provides a resource utilization method of Pueraria lobata root for separating and purifying flavonoids, starch and amino acids, which comprises the following steps:

[0066] 1) Pretreatment: After cleaning the commercially available fresh kudzu root, scrape the skin, cut into thin strips, grind and squeeze the thin kudzu root strips, add tap water while grinding and squeezing to obtain a crude kudzu root slurry;

[0067] 2) Cellulase hydrolysis: adjust the slurry temperature to 37°C, add cellulase to break the cell wall and release the starch and protein in the kudzu root. The added mass of cellulase is 4% of the mass of fresh kudzu root;

[0068] 3) Ultrasonic extraction: Add ethanol to the hydrolyzed slurry, keep the volume concentration of ethanol at 60% and temperature at 60°C, perform ultrasonic extraction, with ultrasonic power of 100W, ultrasonic frequency of 40KHz, and extraction for 30 minutes. Flavonoids are released, and Pueraria lobata extract...

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Abstract

The present invention provides a method for resource utilization of Pueraria lobata for separating and purifying flavonoids, starch and amino acids. , separation of protease, separation and purification of starch, waste residue and waste liquid treatment. The invention combines ultrasonic extraction, ultrafiltration, hydrolysis and other technologies to recover high-purity flavonoids, starches, amino acids and other substances extracted from pueraria through the ultrafiltration of multi-stage membranes. The field process realizes the discharge of wastewater up to the standard and the resource utilization of solid waste, and the process is simple and feasible, the cost is low, the product purity is high, and it is suitable for industrial scale production.

Description

Technical field [0001] The invention relates to the technical field of resource utilization, in particular to a resource utilization method of Pueraria lobata root for separating and purifying flavonoids, starch and amino acids. Background technique [0002] As the concepts of organic food, pollution-free food, and green health food become more popular, the demand and output of food made from characteristic resources such as kudzu powder and kudzu root as raw materials continue to increase. However, in the actual extraction and utilization process of Pueraria lobata root, due to technical reasons, the recycling rate of a large amount of flavonoids, starch and amino acids contained in it is low, resulting in a waste of resources, and the product purity is low and the availability is not high. In addition, although the existing Pueraria lobata resource processing and recycling technologies are of various types and types, they all have their own problems. [0003] The Chinese patent ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/02C12P13/04C07G99/00C07C227/40C07C229/00C02F103/32
CPCC12P13/04C02F3/02C07C227/40C02F2103/32C07G99/00C07C229/00Y02W10/10
Inventor 李柏林赵维鑫张程琛
Owner WUHAN UNIV OF TECH
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