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Application of Ad-sAxl in establishment of preeclampsia rat model and establishment method of preeclampsia rat model

A technology of rat model and establishment method, which is applied in the field of biomedicine, can solve the problems of not responding well to the severity of the disease, and achieve the effect of ensuring reliability, strong reproductive ability, and good controllability of operation

Active Publication Date: 2017-01-11
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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Problems solved by technology

[0005] The present invention aims to solve the problem that in the prior art, the rat model of preeclampsia established by sFlt-1 constructed by injecting adenovirus into the tail vein of rats will not cause damage to multiple organs and viscera, and cannot well reflect the severity of the disease. The problem is to provide a specific method for establishing a rat model of preeclampsia and a rat model of preeclampsia by injecting Ad-sAxl into pregnant mother mice through the tail vein. The rat model of preeclampsia has elevated blood pressure, Changes in the characteristic symptoms and signs of preeclampsia, such as proteinuria and damage to important organs such as the heart, liver, and kidney, are consistent with the symptoms, signs and pathological process of human preeclampsia, and can reflect the systemicity and severity of the disease. Good controllability and stability

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  • Application of Ad-sAxl in establishment of preeclampsia rat model and establishment method of preeclampsia rat model
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  • Application of Ad-sAxl in establishment of preeclampsia rat model and establishment method of preeclampsia rat model

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Embodiment Construction

[0035] The present invention will be described in detail below in conjunction with specific embodiments.

[0036] Main reagents and materials: clean grade SD rats (Chengdu Dashuo Biotechnology Co., Ltd.), recombinant adenovirus vector (Ad-sAxl) synthesized by Shanghai Jikai Gene Company, and urine protein determination (Sigma, USA).

[0037] Main instruments: BP-98A intelligent non-invasive blood pressure-mouse instrument (Beijing Ruanlong Biotechnology Co., Ltd.), BL-420S biological function experiment system (BL series biological function experiment system, Chengdu Taimeng Technology Co., Ltd.), rat metabolic cage (Animal Experiment Center, West China Hospital, Sichuan University), Automatic Hematology Analyzer BC-3000 (Shenzhen Mindray Biomedical Company), Automatic Biochemical Analyzer BS-200 (Shenzhen Mindray Biomedical Company), Scanning Electron Microscope (Japan Olympus Company) ).

[0038] Establishment and identification of preeclampsia rat model:

[0039] (1) Sele...

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Abstract

The invention provides application of Ad-sAxl in the establishment of a preeclampsia rat model and an establishment method of the preeclampsia rat model. The preeclampsia rat model is established by injecting Ad-sAxl into a pregnant rat through in a caudal vein manner and has the preeclampsia characteristic symptoms and symptomatic changes including elevation of blood pressure, injuries of important organs such as proteinuria, heart, liver and kidney, and the characteristic symptoms and symptomatic changes are accordant with preeclampsia symptom, signs and pathological processes of human and can reflect the systematicness and severity of diseases; and the operational controllability and the stability are good. The application can be applied to the research of occurrence and development mechanisms and control measures of preeclampsia.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the application of Ad-sAxl in establishing a preeclampsia rat model and a method for establishing the preeclampsia rat model. Background technique [0002] Preeclampsia is a pregnancy-specific disease characterized by elevated blood pressure and proteinuria after 20 weeks of gestation, which can involve multiple organs throughout the body and seriously threaten maternal and fetal health. The pathogenesis of preeclampsia is not yet clear, but current studies suggest that its occurrence is closely related to early trophoblast invasion disorder and spiral artery remodeling disorder, and extensive vascular endothelial injury in the body is the further development of the disease and presents various degrees. critical aspect of clinical presentation. The imbalance between pro-angiogenic and anti-angiogenic not only locally affects trophoblast invasion and spiral artery remodeling...

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Application Information

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IPC IPC(8): A61K38/45
CPCA61K38/45C12Y207/10001
Inventor 周容刘希婧周盛萍龚云辉王艳云代莉贾瑾
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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