Preparation method and application of nitrogen-doped carbon nano tube composite L-cysteine modified glassy carbon electrode

A nanotube composite and glassy carbon electrode technology, which is applied in the direction of material analysis, measuring devices, and instruments through electromagnetic means, can solve the problems of difficult detection, unsatisfactory results of theophylline and caffeine measurement, etc., and achieve improvement Sensitivity, convenient and fast detection process, and increased current response

Inactive Publication Date: 2017-01-04
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because the oxidation peaks of theophylline and caffeine are very close to each other, it makes the detection difficult
Because the electrochemical modified electrode method has the advantages of fast, accurate, sensitive, and portable, and theophylline and caffeine are both electrically active, the electrochemical modified electrode method is widely used in the detection of these two substances. , but the results of the simultaneous measurement of theophylline and caffeine are not yet satisfactory
The simultaneous detection of theophylline and caffeine using nitrogen-doped carbon nanotubes composited with L-cysteine-modified glassy carbon electrodes has not been reported yet

Method used

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  • Preparation method and application of nitrogen-doped carbon nano tube composite L-cysteine modified glassy carbon electrode
  • Preparation method and application of nitrogen-doped carbon nano tube composite L-cysteine modified glassy carbon electrode
  • Preparation method and application of nitrogen-doped carbon nano tube composite L-cysteine modified glassy carbon electrode

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Embodiment 1

[0035] In this example, see Figure 1~4 , a method for preparing a nitrogen-doped carbon nanotube composite L-cysteine ​​modified glassy carbon electrode, comprising the steps of:

[0036] ⅰ. Pretreatment of glassy carbon electrodes:

[0037] First, the glassy carbon electrode was coated with 0.05 μm Al 2 o 3 Polishing powder and suede are polished to a mirror surface, and then ultrasonically cleaned with distilled water, dilute nitric acid solution, absolute ethanol and double distilled water in sequence, and then used;

[0038] ⅱ. Preparation of nitrogen-doped carbon nanotube modification solution: First, take 2 mg of nitrogen-doped carbon nanotubes and dissolve them in 2 mL of ethylene glycol to prepare a suspension with a concentration of nitrogen-doped carbon nanotubes of 1 mg / mL. After the above suspension was uniform and stable, 500 μL of the dispersed suspension was added to 10 mL of KCl solution with a concentration of 0.1 mol / L by a micro-injector, and ultrasonica...

Embodiment 2

[0053] This embodiment is basically the same as Embodiment 1, especially in that:

[0054] In this embodiment, a method for preparing a nitrogen-doped carbon nanotube composite L-cysteine-modified glassy carbon electrode comprises the following steps:

[0055] ⅰ. The pretreatment of glassy carbon electrode: this step is identical with embodiment one;

[0056] First, the glassy carbon electrode was coated with 0.05 μm Al 2 o 3 Polishing powder and suede are polished to a mirror surface, and then ultrasonically cleaned with distilled water, dilute nitric acid solution, absolute ethanol and double distilled water in sequence, and then used;

[0057] ⅱ. Preparation of nitrogen-doped carbon nanotube modification solution: First, take 1.0 mg nitrogen-doped carbon nanotubes and dissolve them in 2.0 mL ethylene glycol to prepare a suspension with a nitrogen-doped carbon nanotube concentration of 0.5 mg / mL After the above suspension is uniform and stable, use a micro-injector to tak...

Embodiment 3

[0063] This embodiment is basically the same as the previous embodiment, and the special features are:

[0064] In this embodiment, a method for preparing a nitrogen-doped carbon nanotube composite L-cysteine-modified glassy carbon electrode comprises the following steps:

[0065] ⅰ. The pretreatment of glassy carbon electrode: this step is identical with embodiment one;

[0066] First, the glassy carbon electrode was coated with 0.05 μm Al 2 o 3 Polishing powder and suede are polished to a mirror surface, and then ultrasonically cleaned with distilled water, dilute nitric acid solution, absolute ethanol and double distilled water in sequence, and then used;

[0067] ⅱ. Preparation of nitrogen-doped carbon nanotube modification solution: First, take 3.0 mg nitrogen-doped carbon nanotubes and dissolve them in 2.0 mL ethylene glycol to prepare a suspension with a nitrogen-doped carbon nanotube concentration of 1.5 mg / mL After the above suspension is uniform and stable, use a ...

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Abstract

The invention discloses a preparation method and application of a nitrogen-doped carbon nano tube composite L-cysteine modified glassy carbon electrode. The preparation method mainly comprises the following steps: preparing an electrodeposit nitrogen-doped carbon nano tube and an electropolymerization L-cysteine modifed glassy carbon electrode; and performing sensitive quantitative analysis determination on theine and caffeine by use of a differential pulse voltammetry method. Experimental results indicate that in a 0.01mol / L sulfuric acid-sodium sulfate buffer solution with the pH value of 1.70, the modified electrode has obvious catalysis and sensitivity increasing effects on theine and caffeine; under optimum conditions, the differential pulse voltammetry method is adopted for measurement, the concentrations of theine and caffeine are respectively within a range of 0.1-70.0 microns and a range of 0.4-140.0 microns and have good linear relationship with a peak current, and the detection limits respectively reach 0.04 and 0.20 microns. The method is used for measuring contents of theine and caffeine in a theine slow-release tablets and a Coke sample, and the results are satisfactory.

Description

technical field [0001] The invention relates to a preparation method and application of a composite material electrode, in particular to a preparation method and application of a glassy carbon electrode, and is applied in the technical field of detection instruments and detection methods for theophylline and caffeine. Background technique [0002] Theophylline, namely THEO, chemically named 1,3-dimethylxanthine, as a methylpurine drug, is widely used as an adjuvant therapy drug, which has the functions of strengthening the heart, diuresis, dilating coronary arteries, relaxing bronchial smooth muscle and Stimulate the central nervous system and so on. Mainly used for the treatment of bronchial asthma, emphysema, bronchitis, cardiac dyspnea. It is also clinically used as a diuretic, cardiotonic and muscle relaxant, etc. However, the presence of high concentrations of theophylline in the human body will have serious harmful side effects, so the appropriate dose of theophylline...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3277
Inventor 丁亚萍王英子欧阳小茜李丽杨华廖兰凤崔世强
Owner SHANGHAI UNIV
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