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Gene trapping kit for trapping blood disease related gene

A gene capture and kit technology, which is used in the determination/inspection of microorganisms, chemical libraries, combinatorial chemistry, etc., can solve the problems of difficult consistency of sequencing platforms, great influence, and cumbersome background library establishment, and achieve the effect of good detection results.

Active Publication Date: 2017-01-04
ZHEJIANG ANNOROAD BIO TECH CO LTD +2
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This data processing method of introducing the background database has the following disadvantages: 1. The background database is generally obtained from the target gene sequencing data of healthy people, which needs to be established separately, and the cost is high; 2. The background database is subject to the experimental conditions and sequencing platforms when it is established How to ensure the consistency of the experimental conditions and sequencing platform when obtaining the target gene sequencing data and the experimental conditions and sequencing platform when establishing the background database is a difficult problem; 3. For the relatively small preference for whole genome sequencing In terms of data, the establishment of the background library is too cumbersome

Method used

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  • Gene trapping kit for trapping blood disease related gene
  • Gene trapping kit for trapping blood disease related gene
  • Gene trapping kit for trapping blood disease related gene

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Experimental program
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Embodiment 1

[0109] 1.1 Design and synthesis of liquid chip for gene detection of blood diseases

[0110]According to literature reports and information from multiple databases (NCBI-PDB, PubMed, COSMIC, Clinvar, HGMD, GeneCards, zj-LOVD, etc.), the target genes for blood disease detection were selected. May have a partial tandem duplication, located on chromosome 11. The probe is designed for the exon (or 200 bp at the front and rear ends of the exon) of the specific disease-causing gene or a specific target region. The designed probes are compared with databases such as NCBI by Blast, and it is necessary to ensure that the number of bases of the probes is between 60 and 80 nt, the TM value is moderate, there is no palindromic sequence and other special structures, and the sequence homology is within 50 % to 80%, these values ​​are used to ensure the specificity of the probe.

[0111] The designed probe sequences were synthesized into oligonucleotide probes, and after PCR verification, ...

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Abstract

The invention provides a gene trapping kit for trapping a blood disease related gene. The gene trapping kit provided by the invention comprises probes for gene trapping, wherein the probes for gene trapping comprise probes for a target gene and probes for a reference gene. According to the invention, in CNV (copy number variation) detection based on gene trapping and sequencing, a favorable detection result can still be obtained even under the condition that no background database is used in late data processing.

Description

technical field [0001] The invention belongs to the field of molecular in vitro diagnosis, and specifically relates to a gene capture kit especially suitable for in vitro diagnosis of specific structural variations of blood disease-related genes. Background technique [0002] Blood disease is a non-solid tumor, and its gene-related research is in the leading position in cancer, and the detection of blood disease-related genes is also the first to enter clinical application. In recent years, due to the development of molecular biology techniques, the understanding of the molecular genetic changes of blood disease cells has also been deepened. So far, at least dozens of fusion genes have been reported to be involved in blood diseases. It has been recognized that there are chromosomal structural aberrations in most blood diseases, including deletions, duplications, inversions, translocations, etc., which lead to structural variation of proto-oncogenes and tumor suppressor gene...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C40B50/06
CPCC12Q1/6827C12Q2545/101C12Q2565/549
Inventor 梁峻彬荆瑞琳刘伟王秀莉刘卉侯光远玄兆伶李大为陈重建
Owner ZHEJIANG ANNOROAD BIO TECH CO LTD
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