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Human leukocyte antigen gene detection kit and application thereof

A leukocyte antigen and gene detection technology, which is used in the screening of skin adverse drug reactions caused by metronidazole, a human leukocyte antigen gene detection kit, and a human leukocyte antigen gene-HLA-B*39:01 gene detection kit. Can solve problems such as unseen and unseen kit reports

Pending Publication Date: 2017-01-04
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] After searching the prior art literature, it is found that there has been no relevant report about the correlation between the HLA-B*39:01 gene and the drug eruption caused by metronidazole and its use as a gene marker to screen the drug eruption caused by the drug; Related kit reports

Method used

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  • Human leukocyte antigen gene detection kit and application thereof
  • Human leukocyte antigen gene detection kit and application thereof
  • Human leukocyte antigen gene detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Collection and extraction of genes

[0049] Patients with drug eruption caused by metronidazole were from Huashan Hospital Affiliated to Fudan University, Shanghai, China. Among them, the diagnosis of urticaria-type drug eruption needs to meet the clinical requirements of wheal-like rash, which cannot completely subside within 24 hours, and may or may not be accompanied by throat obstruction or paroxysmal abdominal pain; exanthematous drug eruption is a generalized symmetrically distributed red patch , and no mucosal involvement; fixed drug eruptions are single or multiple target lesions, and pigmentation remains after the rash improves (Roujeau JC. Clinical heterogeneity of drug hypersensitivity. Toxicology 209(2), 123–129(2005). S, Rzany B, Stern RS, Shear NH, Naldi L, Roujeau JC. Clinical classification of cases of toxic epidermal necrolysis, Stevens–Johnson syndrome, and erythema multiforme. Arch. Dermatol. 129(1), 92–96(1993)); On the premise of informe...

Embodiment 2

[0050] Example 2: Detection of HLA genotyping

[0051] The present invention adopts PCR-SSO method (recommended Kit-One Lambda, CA, USA) for HLA genotyping; the principle is to amplify the polymorphic region of HLA first, and perform isotope or non-isotope labeling on the PCR product during the amplification process, and then target the PCR amplification. According to the principle of base pairing, a series of oligonucleotide probes are designed and immobilized on the membrane, and finally the PCR product is hybridized with the probes on the membrane, and autoradiography is used to judge the result according to the signal; HLA genotyping is carried out according to the standard steps of the kit : Mix DNA samples, substrates, Taq enzymes, and primers and mix them evenly, add them to the amplification plate, amplify according to the conditions in the kit instructions, and perform hybridization, staining, and plate reading of the amplified products; through HLAFusion software (O...

Embodiment 3

[0052] Example 3: HLA-B*39:01 is related to drug eruption caused by metronidazole

[0053] Odds Ratio (OR) and its 95% confidence interval were calculated by using SPSS16.0, and Haldane’s correction was used when necessary, and chi-square test was used for statistical analysis, and the statistical significance level was set at P less than 0.05;

[0054] The results showed that 9 patients with drug eruption caused by metronidazole were collected, and the clinical features and HLA types of drug eruption caused by metronidazole are shown in Table 1;

[0055] Table 1

[0056]

[0057] The positive ratio of HLA-B*39:01 in patients with drug eruption caused by metronidazole compared with the positive carrier ratio of the normal control group is shown in Table 2; the difference between them is statistically significant.

[0058] Table 2

[0059]

[0060] *The difference is statistically significant (P<0.05).

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Abstract

The invention belongs to the fields of biomedicine and reagent detection, and relates to a human leukocyte antigen gene detection kit and application thereof in screening metronidazole-induced adverse drug reactions of skin. The kit contains a reagent for detecting human leukocyte antigen gene HLA-B*39:01 nucleic acid or protein, and amplification primers or a labeled probe of the human leukocyte antigen gene or a specific antibody containing the human leukocyte antigen. The HLA-B*39:01 gene can be used as a labeled gene for predicting metronidazole-induced epispasis. The detection kit can be used for further preparing or evaluating a metronidazole-induced epispasis screening kit, thereby providing valuable reference data for instructing clinical medication, and reducing the possibility of metronidazole-induced epispasis; or the detection kit can also be used for preparing or evaluating therapeutic drugs and especially targeted drugs for metronidazole-induced epispasis, thereby preventing the interactions between the drugs and metronidazole or metabolites thereof in disease development.

Description

technical field [0001] The invention belongs to the field of biomedicine and reagent detection, and relates to a human leukocyte antigen gene detection kit, specifically, to a human leukocyte antigen gene-HLA-B*39:01 gene detection kit, and its use for screening formazan The use of adverse skin drug reactions caused by nitrazole. Background technique [0002] Drug eruption refers to the inflammatory reaction of skin and mucous membranes caused by drugs entering the human body through various channels. Usually, in clinical practice, drug eruptions are divided into common drug eruptions and severe drug eruptions according to the severity of the onset; among them, common drug eruptions mainly include: exanthematous drug eruptions, fixed type drug eruptions, and urticaria type drug eruptions (Roujeau JC. Clinical heterogeneity of drug hypersensitivity. Toxicology 209(2), 123–129(2005)). The onset of drug eruption is acute, and the rash is widely distributed, which seriously af...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/106
Inventor 骆肖群邢清和徐金华杨凡萍陈圣安贺林
Owner FUDAN UNIV
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