Enzyme for producing sea cucumber oligopeptide and enzymolysis technology

A sea cucumber oligopeptide, enzymatic hydrolysis technology, applied in the direction of peptidase, enzyme, pancreatin, etc., can solve the problems that affect the popularization and use, and the taste of the enzymatic hydrolysis solution affects the taste, so as to achieve the product with small molecular weight, short enzymatic hydrolysis time, and product taste Good results

Inactive Publication Date: 2017-01-04
大连平岛天然产物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the enzymatic hydrolyzate produces a more serious bitter taste, which affects the mouthfeel, and affects the popularization and use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Take an appropriate amount of sea cucumber, remove the viscera, wash it, cut it into strips, and grind it twice with a meat grinder. It is better if it can be ground into a slurry with a colloidal grinder. Weigh 100g of crushed sea cucumber or slurry, add 500ml of purified water, and stir evenly. Add NaHSO to feed liquid 3 3mmol / L, heated to 65°C, adjusted the pH to 9.0 with 10% NaOH solution, and stirred for 20min. Adjust the pH of the treated feed liquid to 8.2, cool down to 47°C±1°C, add Ca 2+ 3mmol / L, stir evenly. Add 0.1% compound protease (total amount of protein) to the feed solution, and stir for 3 hours for enzymatic hydrolysis. Adjust the pH of the enzymolysis solution to 4.5-4.6, raise the temperature to 65°C-70°C to inactivate the enzyme, and cool down. Add 95% ethanol to the enzymolysis solution until the ethanol concentration of the mixed solution reaches 60%-65%, to precipitate polysaccharides. Let it stand for more than 2 hours, centrifuge, and the ...

Embodiment 2

[0030] Take an appropriate amount of salted sea cucumber, desalinate it in running water for about 6-8 hours, and take it out. Desalinated sea cucumbers were obtained by refining and soaking in pure water for 2-3 times for 2 hours each time. Chop the desalted sea cucumber into small pieces in a chopping machine, and weigh 10 kg of sea cucumber grains. The sea cucumber grains are ground into a slurry through a colloid mill under running water of pure water. Adjust sea cucumber grains with pure water: pure water = 1:5, and stir evenly. Add Na to the slurry with stirring 2 SO 3 4mmol / L, stirred and dissolved evenly. Heat the feed solution to 70°C, adjust the pH to 9.5 with NaOH solution, and stir for 30 minutes. Cool the feed solution to 47°C±1°C, adjust the pH to 8.5±0.1, and add 0.3mmol / L of L-cysteine. Add 0.12% compound protease (total amount of protein) to the feed liquid, and stir for 3.2 hours for enzymatic hydrolysis. Adjust the pH of the enzymolysis solution to 4....

Embodiment 3

[0032] Take an appropriate amount of lightly dried sea cucumber, soak it in pure water for more than 24 hours, until there are no lumps in the sea cucumber. Take out the soaked sea cucumber and chop it into small pieces in a chopping machine. Weigh sea cucumber grains 100kg. Grind the sea cucumber granules into a slurry through a colloid mill under pure water flow, put it into an enzymatic hydrolysis tank, first add water for soaking sea cucumbers (there are water-soluble proteins, polysaccharides, etc. in the water), and then use pure water to adjust the material: water =1:5, stir evenly. Na was added to the slurry under stirring 2 S 2 o 3 5mmol / L, stirred and dissolved evenly. Heat the feed solution to 75°C, adjust the pH to 10.0 with NaOH solution, and stir for 40 minutes. Cool the feed solution to 48°C±1°C, adjust the pH to 8.6±0.1. Join Ca 2+ 0.4mmol / L, L-cysteine ​​salt 0.5mmol / L. Add 0.15% compound protease (total amount of protein) to the feed solution, and st...

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PUM

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Abstract

The invention discloses an enzyme for producing sea cucumber oligopeptide and an enzymolysis technology, and belongs to the technical field of the sea cucumber oligopeptide production technology. On the basis of selecting a single enzyme, a special compound protease for sea cucumber enzymolysis comprising four enzymes such as incision enzymes and excision enzymes is formed by virtue of optimization; an enzyme activator is added into the compound protease; and after the temperature and PH are adjusted, sea cucumber slurry is preprocessed; and an appropriate amount of preprocessing agent is added before the preprocessing. The enzymolysis technology is reasonable in design, less in enzyme consumption, short in enzymolysis time, and high in enzymolysis yield. A product is good in taste, substantially no bitter or slightly bitter and little in fishy smell.

Description

technical field [0001] The invention relates to the technical field of sea cucumber oligopeptide production technology, in particular to an enzyme and an enzymolysis process for sea cucumber oligopeptide production. Background technique [0002] Sea cucumber is a traditional health recipe in my country. Yao Kecheng, the author of "Food Materia Medica" published in the Ming Dynasty, paid out that sea cucumber has the health-preserving functions of replenishing vitality, nourishing internal organs and eliminating deficiency and damage [1994: 695]. In the Qing Dynasty, "Materia Medica Congxin", "Compendium of Materia Medica Supplements" and other traditional Chinese medicine classics listed sea cucumber as a tonic drug [1955: 495]. [0003] According to the summary of Wu Yiluo, Zhao Xuemin, etc.: Sea cucumber tastes warm, sweet and salty, and enters the heart and kidney meridian. It has multiple functions such as generating blood in all veins, tonifying kidney and nourishing ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C12N9/50C12N9/94C12N9/56C12N9/62
CPCC12N9/54C12N9/62C12N9/6472C12N9/94C12P21/06C12Y304/15001C12Y304/21014C12Y304/22002
Inventor 黄磊
Owner 大连平岛天然产物科技有限公司
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