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Method for preparing enteromorpha oligosaccharide by utilizing naringinase

A technology of Enteromorpha oligosaccharides and naringinase, which is applied in the field of active ingredient extraction, can solve problems such as naringinase Enteromorpha polysaccharides that have not yet been discovered, and achieve the effects of promoting rapid growth, high yield, and high nutritional content

Active Publication Date: 2017-01-04
青岛海大生物集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, no report on the use of naringinase in the preparation of Enteromorpha polysaccharides has been found

Method used

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  • Method for preparing enteromorpha oligosaccharide by utilizing naringinase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take 10kg of fresh Enteromorpha enteromorpha with a water content of 90%, put it into the extraction tank after being pulverized by a wet material grinder, start stirring, add 17mL of 1mol / L hydrochloric acid solution, stir evenly, detect the pH is 5.1, heat up to 50°C, add 400U cellulase, keep stirring and control the temperature at 50°C, within the range of pH 5.0, after 3 hours, add 11mL of 1mol / L hydrochloric acid solution, stir evenly, the detected pH is 4.3, heat up to 55°C, add 300U naringinase , keep stirring and temperature control at 55°C, within the range of pH 4.3, enzymatic hydrolysis for 10 hours. Raise the temperature to 95°C and keep it warm for 15 minutes to inactivate the enzyme activity. Plate and frame filter the enzymolysis solution (750 mesh filter cloth) to obtain 7L filtrate, add 7L tap water to dilute, and pass through the nanofiltration membrane with a molecular weight cutoff of 3500Da, and the effluent 13.5L passes through the nanofiltration m...

Embodiment 2

[0034] Grind the dried Enteromorpha with a pulverizer, pass through a 20-mesh sieve, put 15kg into the extraction tank, add 90L of tap water, start stirring, add 21mL of concentrated hydrochloric acid, stir evenly, detect the pH is 4.9, raise the temperature to 50°C, add 8000U of cellulose Enzyme, keep stirring and temperature control at 50°C, within the range of pH 5.0, extract for 2 hours, then add 16mL concentrated hydrochloric acid, stir evenly, the detected pH is 4.4, heat up to 55°C, add 4200U naringinase, continue stirring and control the temperature at 55°C ℃, pH 4.3 range, enzymatic hydrolysis for 12 hours. Raise the temperature to 95°C and keep it warm for 15 minutes to inactivate the enzyme activity. Plate and frame filtration of the enzymolysis solution (750 mesh filter cloth) to obtain 85L filtrate, add 80L tap water to dilute, and pass through a nanofiltration membrane with a molecular weight cut-off of 3500Da, the effluent 147L passes through a nanofiltration me...

Embodiment 3- Embodiment 8

[0036] Enteromorpha oligosaccharides were prepared according to the parameters described in Table 1. Except for the following parameters, the preparation methods of Example 3-Example 5 were the same as Example 1, and Example 6-Example 8 were the same as Example 2.

[0037] Main parameter in table 1 embodiment 3-embodiment 8

[0038]

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PUM

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Abstract

The invention relates to a method for preparing enteromorpha oligosaccharide by utilizing naringinase and belongs to the field of extraction of active ingredients. Aiming at overcoming the technical disadvantages in the prior art that the yield of the enteromorpha oligosaccharide is relatively low and the content of undegraded polysaccharides in a final product is high, the invention provides the method for preparing the enteromorpha oligosaccharide by utilizing the naringinase through taking enteromorpha as a raw material. The method applies the naringinase to a preparation method of the enteromorpha oligosaccharide for the first time; two sections of enzymolysis are carried out by adopting cellulase and the naringinase and then a concentrated solution is obtained through a nano-filtration membrane; the concentrated solution is dried to obtain the enteromorpha oligosaccharide. The preparation method provided by the invention has the advantages that the yield of the enteromorpha oligosaccharide is high, the content of impurities and the undegraded polysaccharides in the product is low and the product is very suitable for being further processed into functional agricultural products, feed additives, food additives and the like.

Description

technical field [0001] The invention relates to a method for preparing Enteromorpha oligosaccharides by using naringinase, in particular to a method for preparing Enteromorpha oligosaccharides by using Enteromorpha as a raw material and using cellulase and naringinase for two-step enzymatic hydrolysis, which belongs to the extraction of active components field. Background technique [0002] Enteromorpha is a large green algae, commonly known as moss strips, green seaweed, etc. It is an algae plant of the Chlorophyta Ulva, Ulvaceae, and Enteromorpha. It often grows on rocks in the intertidal zone and is widely distributed along the coast of my country. Abundant resources. Enteromorpha is rich in polysaccharides, the content of which can reach more than 40% of the dry weight of Enteromorpha, and the soluble polysaccharides are mainly composed of rhamnose and glucose. Studies have shown that Enteromorpha oligosaccharides prepared from soluble polysaccharides in Enteromorpha ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14A23K20/163A23L29/30
CPCA23V2002/00C12P19/14A23V2200/30A23V2200/324
Inventor 单俊伟台文静但丽霞王海华郝晓晨宿露译
Owner 青岛海大生物集团股份有限公司
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