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Hybridoma cell strain NaN-1 secreting monoclonal antibody resistant to sulfonamide antibiotics (SAs) and application thereof

A hybridoma cell line and monoclonal antibody technology, applied in the field of food safety immunology detection, can solve the problems of expensive instruments, cumbersome pretreatment, inability to detect a large number of samples at the same time, etc., and achieve the effect of good detection sensitivity

Active Publication Date: 2017-01-04
JIANGNAN UNIV
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the sample pretreatment of the instrumental analysis method is cumbersome, time-consuming, expensive instruments, and requires professional operation, it is impossible to realize the simultaneous detection of a large number of samples

Method used

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  • Hybridoma cell strain NaN-1 secreting monoclonal antibody resistant to sulfonamide antibiotics (SAs) and application thereof
  • Hybridoma cell strain NaN-1 secreting monoclonal antibody resistant to sulfonamide antibiotics (SAs) and application thereof
  • Hybridoma cell strain NaN-1 secreting monoclonal antibody resistant to sulfonamide antibiotics (SAs) and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Preparation of hybridoma cell line NaN-1

[0023] 1. Antigen synthesis

[0024] 500 mg aminothiazole acetic acid was dissolved in 7.5 mL of anhydrous methanol, and 400 mg of thionyl chloride was added to the solution. After the reaction solution was refluxed for 2 hours, the volatiles were removed by rotary evaporation, the residue was neutralized with saturated sodium bicarbonate solution, and then extracted with ethyl acetate, and then rotary evaporated to obtain Intermediate I, 300 mg Intermediate I and 600 mg 4-acetamido Benzenesulfonyl chloride was dissolved in 15 mL of pyridine and reacted at room temperature for 3 hours. The reaction solution was concentrated to obtain Intermediate II. 134 mg Intermediate II was dissolved in 5 mL of 1M sodium hydroxide solution, heated to 80°C, and protected by nitrogen. Reaction for 2h. After the reaction, the volatiles were evaporated, and the residue was neutralized with hydrochloric acid, and then extracted twice with...

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Abstract

The invention provides a hybridoma cell strain NaN-1 secreting a monoclonal antibody resistant to sulfonamide antibiotics (SAs) and an application thereof, belonging to the field of food safety immunodetection. The hybridoma cell strain NaN-1 secreting the common monoclonal antibody capable of identifying 25 SAs is collected in the China General Microbiological Culture Collection Center (CGMCC), with collection number of CGMCC No.12028. The monoclonal antibody secreted by the cell strain NaN-1 can identify 25 SAs, has better detection sensitivity, enables the IC<50> of sulfadiazine (SD), sulfamerazine (SMR), sulfadimethoxine (SDM) and sulfathiazole (STZ) to be respectively 4.51mu g / L, 1.80mu g / L, 1.98mu g / L and 0.53mu g / L and can be used for multi-residue detection of SAs in food safety.

Description

Technical field [0001] A hybridoma cell line NaN-1 which secretes monoclonal antibodies against sulfa antibiotics and its application relates to hybridoma cell lines NaN-1 and the monoclonal antibodies against sulfa antibiotics produced therefrom, belonging to the field of food safety immunological detection. Background technique [0002] Sulfonamide antibiotics (SAs) are artificially synthesized antibacterial drugs that have been used clinically for nearly 50 years. They have the advantages of broad antibacterial spectrum, stable properties, and easy use. Such antibiotics are also a class of antibacterial drugs widely used in livestock and aquaculture production. They are often used to prevent and treat diseases of livestock and poultry, and as feed additives to promote animal growth. However, due to its potential harm to the human body and the emergence of drug resistance to pathogenic bacteria, its residue in food has attracted widespread attention. In recent years, various c...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/44G01N33/577C12R1/91
CPCC07K16/44G01N33/577
Inventor 徐丽广陈燕妮胥传来匡华刘丽强宋珊珊吴晓玲胡拥明
Owner JIANGNAN UNIV
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