Cinnamomum longepaniculatum tissue culture method

A tissue culture and oil camphor technology, applied in horticultural methods, botanical equipment and methods, climate change adaptation and other directions, can solve the problems of low success rate of oil camphor induction, no growth, browning and death, etc., to ensure the oil content and The effect of growth rate, fast growth rate and stable species

Active Publication Date: 2017-01-04
苏州金铁红林业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Nowadays, although many plants are propagated by tissue culture, there is no report on the successful reproduction of oil camphor by tissue culture. The main reasons why oil camphor tissue culture is difficult to succeed are:
[0004] 1. The content of camphor oil such as eucalyptol, myristene, camphor, and abietol contained in camphor leaves is 1 to 2 times that of camphor leaves, which makes the explants easily brown during tissue culture. one of the reasons for the failure of tissue culture;
[0005] 2. There is no suitable tissue culture medium for Cinnamomum oleifer...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: a kind of method of oil camphor tissue culture, comprises the following steps:

[0030]1. Induction culture: use a scalpel and tweezers to cut the camphor explants after surface sterilization into 0.8-1.2 cm long stem segments, each stem segment has an axillary bud, and inoculate it into the starting medium by oblique insertion Above, the temperature of the culture room is controlled at 26°C±2°C, the light intensity is 1500-2000lx, the light time is 10-12h per day, and the culture time is 30d-40d; The young shoots of the year selected on the bar;

[0031] 2. Subculture: Cut the subcultured seedlings into bud clusters or stem segments with axillary buds and inoculate them on the subculture medium. Inoculate no less than 5 bud clusters or stem segments in each bottle, and the number of buds in each bud cluster should be at least 3 Every 30d-40d, the subcultured seedlings were transferred to the new subculture medium, the number of subculture generations was...

Embodiment 2

[0038] Embodiment 2: a kind of method for the tissue culture of oil camphor, comprising four steps of induction culture, proliferation culture, rooting culture, seedling hardening, the used initiation medium of described induction culture step, the subculture used in proliferation culture step Both the medium and the rooting medium used in the rooting culture step contain improved MS medium; the improved MS medium adds 1430 mg / L of NaH to the macroelements of the original MS medium 2 PO 4 , and change the KNO 3 The concentration is 1600mg / L, NH 4 NO 3 Concentration is 720mg / L, all the other substances remain unchanged; described improved MS culture medium adds calcium pantothenate 1.2mg / L in the original MS culture medium organic matter, and changes pyridoxine hydrochloride concentration to 2.0mg / L, thiamine hydrochloride The concentration is 3mg / L, the concentration of niacin is 10mg / L, and the other substances remain unchanged. After hardening, the seedlings can be transp...

Embodiment 3

[0044] Embodiment 3: a kind of method of tissue culture of camphor oleifera, comprises four steps of induction culture, proliferation culture, rooting culture, seedling hardening, the used initiation medium of described induction culture step, the subculture used in proliferation culture step Both the medium and the rooting medium used in the rooting culture step contain improved MS medium; the improved MS medium adds 1430 mg / L of NaH to the macroelements of the original MS medium 2 PO 4 , and change the KNO 3 The concentration is 1600mg / L, NH 4 NO 3 Concentration is 720mg / L, all the other substances remain unchanged; described improved MS culture medium adds calcium pantothenate 1.2mg / L in the original MS culture medium organic matter, and changes pyridoxine hydrochloride concentration to 2.0mg / L, thiamine hydrochloride The concentration is 3mg / L, the concentration of niacin is 10mg / L, and the other substances remain unchanged.

[0045] During the steps of induction cultu...

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Abstract

The invention belongs to the technical field of woody plant vegetative propagation and particularly relates to a cinnamomum longepaniculatum vegetative propagation method, in particular to a cinnamomum longepaniculatum tissue culture method. The cinnamomum longepaniculatum tissue culture method comprises the steps of induction culture, propagation culture, rooting culture and seedling hardening. The success rate of cinnamomum longepaniculatum tissue culture can be effectively increased through an improved MS culture medium, designed for cinnamomum longepaniculatum, in culture media for induction culture, propagation culture and rooting culture, a large quantity of cinnamomum longepaniculatum seedlings uniform in cinnamomum longepaniculatum oil content and specification and stable in species nature can be produced in a short period, and a method is provided for quick and high-quality forestation of cinnamomum longepaniculatum.

Description

technical field [0001] The invention relates to the technical field of asexual propagation of woody plants, in particular to a method for asexual propagation of camphor oleifera. Background technique [0002] Cinnamomum oleifera is a precious tree species of the genus Cinnamomum lauraceae, and Cinnamomum oleifera can be used to extract camphor oil, which has broad market prospects. Nowadays, most of the propagation methods of oil camphor are propagated by seeds, cuttings or layering. But because the camphor seed contains a large amount of oil, it is easy to lose the germination ability, causing the success rate of seed propagation to be very low. The yield and quality are not high, affecting the economic benefits after afforestation. And when adopting the method for cutting or layering to propagate, because camphor is a difficult rooting plant, the success rate is not high, and the camphor seedlings grown by cutting or layering grow slowly, have poor tree shape and lower y...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02A40/22Y02P60/40
Inventor 翁春桃杨军钟丽萍
Owner 苏州金铁红林业科技有限公司
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