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Method for preparing functional feed additive through biological glycolysis of enteromorpha

A feed additive and functional technology, applied in the field of biological fermentation, can solve the problems of no reports and patents, and achieve the effects of increasing utilization value, improving digestibility, increasing feed palatability and feeding rate

Inactive Publication Date: 2017-04-26
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] According to the relevant literature and books consulted by the inventor, there is no report or patent on the synergy of enzymolysis and fermentation to transform high-concentration Enteromorpha under aerobic conditions to prepare functional feed additives related to this patent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation process of Enteromorpha functional feed additives is as follows.

[0022] 1. Pre-treatment: The fresh Enteromorpha is homogenized with a colloid mill, and the tooth gap is 20μm.

[0023] 2. Liquid transformation and synergistic fermentation of high-concentration Enteromorpha: Add 9% (mass to volume ratio) of pretreated Enteromorpha (calculated by dry weight) to the water, after heating and boiling, adopt three-stage control: First In the stage, add pretreated Enteromorpha dry weight 0.6% (based on the dry weight of the mass-volume ratio) compound enzyme, the cellulase and β-amylase ratio is 3:1 (based on the volume ratio), control pH 5.5, The temperature is 45℃, the stirring speed is 100 rpm, and the hydrolysis is 4 h; the second stage, supplement nutrient salt, inoculate 10% of Alternomonas for liquid transformation, control pH 6.5, temperature 30℃, stirrer speed 200 rpm, dissolved oxygen 70 %-100%, maintain for 10 hours; in the third stage, adjust the temp...

Embodiment 2

[0027] The preparation process of Enteromorpha functional feed additives is as follows.

[0028] 1. Pre-treatment: Dry entero moss powder is pulverized with a jet type ultrafine pulverizer, and the particle size distribution range is 100-200 mesh.

[0029] 2. Liquid transformation and synergistic fermentation of high-concentration Enteromorpha: Add 12% (mass-volume ratio) of pre-treated Enteromorpha to the water. After heating and boiling, a three-stage control is adopted: the first stage is to add the pretreatment of Enteromorpha 0.4% of the dry weight of the moss, the ratio of cellulase to β-amylase is 6:1 (in terms of volume ratio), pH 6.0, temperature 55°C, stirring speed 50 rpm, hydrolysis for 4 hours; second stage, supplement nutrition Salt (the composition is the same as in Example 1), inoculate 15% Alternomonas for liquid transformation, control pH 6.0, temperature 32°C, stirrer speed 150 rpm, dissolved oxygen 70%-100%, maintain for 10 h; In the third stage, adjust the tem...

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PUM

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Abstract

The invention discloses a method for preparing a functional feed additive through biological glycolysis of enteromorpha. The method comprises the main steps of performing pretreatment, performing high-concentration enteromorpha liquid transformation and synergistic glycolysis, and preparing the functional feed additive. According to the method disclosed by the invention, the extraction of nutritive substances and functional factors of enteromorpha, and the biological transformation of active functional components are realized, so that the enteromorpha functional feed additive is prepraed. The method disclosed by the invention is simple, convenient and easy in technology and high in production efficiency, in the raising process, the ingestion digestibility of aquaculture animals of trepangs, abalones, prawns, fish and the like can be effectively increased, the breeding cycle can be shortened, and economic benefits are increased.

Description

Technical field [0001] The invention relates to a preparation method of Enteromorpha feed additive, in particular to a method for preparing a biologically fermented Enteromorpha functional feed additive, and belongs to the technical field of biological fermentation. Background technique [0002] Feed additives are small or trace substances added during the production, processing and use of feeds. They can strengthen the nutritional value of basic feeds, save feed costs, and improve the growth quality of the tested animals. In recent years, with the rapid development of biological fermentation technology and industry, many of its products and by-products have gradually been used in production and life. [0003] However, traditional terrestrial plant additives have a single nutrient composition, and the types of protein and other nutrients cannot fully meet the growth needs of the animal body. As a major category of marine resources, seaweeds have a good complementary effect on nutr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/30A23K10/14A23K10/12A23K40/00
Inventor 王鹏乔乐克迟永洲
Owner OCEAN UNIV OF CHINA
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