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Kit for induced amplification of regulatory T cells from naive T cells

A technology of cell induction and kit, applied in the direction of cell culture active agents, animal cells, vertebrate cells, etc., can solve the problems of long amplification induction cycle, limited clinical application, biosafety issues, etc., and achieve short amplification induction cycle , cost reduction, and easy operation

Inactive Publication Date: 2016-12-21
SHENZHEN ZHONGSHAN INST OF GENETICS & REPRODN
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, nTreg only accounts for normal peripheral blood CD4 + 5‐15% of T cells, greatly limiting its clinical application
Most of the existing kit products used to induce and expand Treg have the following problems: using anti‐CD3 / CD28‐magnetic beads as the inducer, the cost of amplification is high; using heterogeneous or heterologous serum has potential biosafety problems ;Requires multiple sorting, complex operation and increased cost; in addition, there are other problems such as long amplification induction period and poor stability

Method used

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  • Kit for induced amplification of regulatory T cells from naive T cells
  • Kit for induced amplification of regulatory T cells from naive T cells
  • Kit for induced amplification of regulatory T cells from naive T cells

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Embodiment Construction

[0021] The technical solutions of the present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments, but the present invention is not limited to the following embodiments. Unless otherwise specified, the reagents and instruments used in the examples of the present invention are all known and available in the art.

[0022] The kit for inducing and expanding regulatory T cells from naive T cells of the present invention comprises the following components: anti-CD3 antibody, PBS buffer, lymphocyte separation fluid, CD4 + T Cell Biotin‐Antibody Cocktail II antibody, Naive CD4 + T Cell Micro Bead Cocktail II magnetic beads, Treg induction medium. Wherein, preferably, the formula ratio of the Treg induction medium is: RPMI1640 medium to which 2.06mM Glutamax-I and 25mM HEPES buffer have been added, 10% v / v autologous serum is added to the RPMI 1640 medium, And add the final concentration of 200U / ml recombinant ...

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Abstract

The invention provides a kit for induced amplification of regulatory T cells from naive T cells. The kit comprises an anti-CD3 antibody, a PBS (phosphate buffer solution), a lymphocyte separation solution, a naive CD4<+>T Cell Biotin-Antibody Cocktail II antibody, Naive CD4[+]T Cell Micro Bead Cocktail II magnetic beads and a Treg induced culture medium. The invention also provides an application method of the kit. The kit and application method thereof are simple to operate, are safe and effective, and have the advantages of shorter amplification induction period, higher stability and lower cost.

Description

technical field [0001] The invention belongs to the field of molecular bioengineering, and relates to a kit, in particular to a kit for inducing and expanding regulatory T cells from naive T cells, and a method for using the kit. Background technique [0002] Immune tolerance refers to an active non-response state displayed by immune active cells when exposed to antigenic substances, which is a biological phenomenon different from immunodeficiency and immunosuppression. Immune intolerance is associated with many diseases, such as autoimmune diseases, graft versus host disease (GVHD) and maternal-fetal immune rejection. In organ or tissue transplantation, although traditional immunosuppressive drugs can significantly prolong the survival time of grafts, their side effects are decreased overall immune function of patients, increased chances of infection, and may induce tumors, etc., which greatly limit the clinical organ transplantation. Further development of transplantation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783
CPCC12N5/0637C12N2501/15C12N2501/2302C12N2501/51C12N2501/515C12N2501/999
Inventor 黄春宇刁梁辉张涛陈现何来宾林嘉音李振莲许健
Owner SHENZHEN ZHONGSHAN INST OF GENETICS & REPRODN
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