Culture medium for adipose derived stem cells

An adipose-derived stem cell and culture medium technology, applied in cell culture active agent, culture process, tissue culture and other directions, can solve the problems of unsatisfactory cell proliferation rate, poor cell adherence, pollution, etc., to promote rapid and massive proliferation and maintain cells. Active, cost-reducing effect

Inactive Publication Date: 2016-12-14
ANHUI HUIEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The adipose stem cell medium in the prior art often uses fetal bovine serum. Due to the use of animal-derived fetal bovine serum, the stem cell medium is mixed with non-human substances, resulting in residue or pollution, and the existing adipose stem cell medium exists Poor cell adhesion and unsatisfactory cell proliferation speed

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0015] A culture medium for adipose stem cells proposed by the present invention includes RPMI-1640 medium, and also includes: D-glucose 2g / L, cholesterol 45mg / L, potassium chloride 0.5g / L, sodium selenite 0.3μg / L, taurine 8μg / L, heparin calcium 20μg / L, human serum albumin 2.2g / L, vascular endothelial cell growth factor 50mg / L, fibroblast growth factor 3mg / L, platelet-derived growth factor 80mg / L, Glutathione 20mg / L, vitamin 100mg / L, plant extract 100mg / L.

Embodiment 2

[0017] A kind of medium that the present invention is used for adipose stem cell comprises RPMI-1640 medium, also comprises: D-glucose 2.5g / L, cholesterol 30mg / L, potassium chloride 0.8g / L, sodium selenite 0.1 μg / L, taurine 10μg / L, heparin calcium 10μg / L, human serum albumin 2.5g / L, vascular endothelial cell growth factor 20mg / L, fibroblast growth factor 6mg / L, platelet-derived growth factor 40mg / L , glutathione 40mg / L, vitamin 50mg / L, plant extract 200mg / L.

[0018] The plant extracts include by weight: 1 part of berberine, 17 parts of jujube polysaccharide, 4 parts of coix seed polysaccharide, 15 parts of ginsenoside, and 3 parts of methanol extract of Gorgon fruit.

Embodiment 3

[0020] A culture medium for adipose stem cells proposed by the present invention includes RPMI-1640 medium, and also includes: D-glucose 2.4g / L, cholesterol 35mg / L, potassium chloride 0.7g / L, sodium selenite 0.15 μg / L, taurine 9.5μg / L, heparin calcium 12μg / L, human serum albumin 2.4g / L, vascular endothelial cell growth factor 30mg / L, fibroblast growth factor 5mg / L, platelet-derived growth factor 50mg / L L, glutathione 35mg / L, vitamin 60mg / L, plant extract 180mg / L.

[0021] The plant extracts include by weight: 3 parts of berberine, 15 parts of jujube polysaccharide, 6 parts of coix seed polysaccharide, 13 parts of ginsenoside, and 5 parts of methanol extract of Gorgon fruit.

[0022] The vitamins include by weight: 16 parts of vitamin C, 21 parts of vitamin B group, and 11 parts of vitamin E; the vitamin B group is a combination of riboflavin, pantothenic acid, cyanocobalamin and biotin.

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Abstract

The invention discloses a culture medium for adipose derived stem cells. The culture medium for the adipose derived stem cells comprises RPMI-1640 medium, and further comprises 2 to 2.5g / L of D-glucose, 30 to 45mg / L of cholesterol, 0.5 to 0.8g / L of potassium chloride, 0.1 to 0.3 mu g / L of sodium selenite, 8 to 10 mu g / L of taurine, 10 to 20 mu g / L of heparin calcium, 2.2 to 2.5g / L of human serum albumin, 20 to 50mg / L of vascular endothelial growth factors, 3 to 6mg / L of fibroblast growth factors, 40 to 80mg / L of platelet-derived growth factors, 20 to 40mg / L of glutathione, 50 to 100mg / L of vitamins, and 100 to 200mg / L of plant extracts. The culture medium for the adipose derived stem cells disclosed by the invention is free of components of animal origin, has no residues or pollution, has good cell anchorage dependence and high proliferation rate, improves the pathogenic microorganism resistance of the culture medium, and further lowers the cost of the culture medium.

Description

technical field [0001] The invention relates to the technical field of stem cell culture medium, in particular to a culture medium for fat stem cells. Background technique [0002] The adipose stem cell medium in the prior art often uses fetal bovine serum. Due to the use of animal-derived fetal bovine serum, the stem cell medium is mixed with non-human substances, resulting in residue or pollution, and the existing adipose stem cell medium exists Cell adhesion is poor, cell proliferation rate is not ideal and so on. Contents of the invention [0003] Based on the technical problems in the background technology, the present invention proposes a culture medium for adipose stem cells, which has no animal-derived components, does not cause residue or pollution, has good cell adhesion, fast proliferation, and improves the disease resistance of the culture medium. The role of the original microorganisms, while reducing the cost of the medium. [0004] A culture medium for adi...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N2500/12C12N2500/30C12N2500/34C12N2500/38C12N2500/76C12N2500/84C12N2501/10C12N2501/165C12N2501/91
Inventor 张正亮
Owner ANHUI HUIEN BIOTECH
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