A microfluidic chip for in situ control culture of animal tissues

Abstract

The invention provides a micro-fluidic chip for in-situ contrast culture of animal tissues. The micro-fluidic chip is provided with a micro-fluidic network structure and a plurality of fluid inlets, wherein the micro-fluidic network structure adopts an array type and is used for culturing the animal tissues; the flow inlets are connected with a tissue culture array; by utilizing the laminar state of fluid from the inlets in a micro-fluidic passage and the gathering function of a tissue suspension from the middle inlet, the tissues are distributed to each row of culture units by multiple levels of Y-shaped flow separating areas, and each culture unit is used for fixing, culturing and observing the tissues; the different types of culture liquids are inputted by different inlets, the flow rate is adjusted, the tissues fixed in different rows are cultured in different types of culture liquids, and then the in-situ contrast culture of tissues on the same micro-fluidic chip is realized. The micro-fluidic chip has the advantage that by utilizing transparent material, the real-time monitoring under a microscope and the in-situ analysis of dynamic growth and differentiation process of tissues can be performed, so as to study the dynamics parameters of structure and function of animal tissues under the condition of stimulation by different culture environments.

Description

technical field [0001] The invention provides a microfluidic chip for in-situ control culture of animal tissues, which belongs to the technical fields of microfluidic technology and animal tissue culture analysis. technical background [0002] Animal tissue culture refers to taking out tissues or organs from animals, simulating the physiological environment conditions in the body, and making the isolated tissues or organs survive, grow, and maintain their differentiation, structure and ( or) function is an important technical basis of modern animal tissue engineering. Animal tissue culture has extensive scientific research and practical application value in biological, medical and pharmaceutical fields such as three-dimensional tissue and organ engineering, embryonic stem cell engineering, animal cloning, large-scale preparation of animal cell products, and in vitro drug testing between cells and animals. [0003] Traditional animal tissue culture techniques usually use cul...

AI Technical Summary

Problems solved by technology

In addition, the current tissue culture chips are not highly integrated and scalable

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