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Preparation method of pumpkin polysaccharide hydrogel composite with hypoglycemic effect

A composite hydrogel and pumpkin polysaccharide technology, which can be applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, drug combinations, etc. Glue and other problems, to achieve the effect of reducing the main dosage, making up for the small amount of drug loading, and a wide range of raw material sources

Active Publication Date: 2016-12-14
甘肃中科博瑞生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the small amount of drug loaded and unable to meet the needs of drug efficacy is the current restrictive factor for the development of sustained-release preparations. Therefore, how to reduce the drug-loaded amount of sustained-release preparations under the premise of ensuring drug efficacy has become the focus of current research.
No reports of hydrogels with hypoglycemic activity have been seen so far

Method used

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  • Preparation method of pumpkin polysaccharide hydrogel composite with hypoglycemic effect
  • Preparation method of pumpkin polysaccharide hydrogel composite with hypoglycemic effect
  • Preparation method of pumpkin polysaccharide hydrogel composite with hypoglycemic effect

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preparation example Construction

[0012] The present invention is the preparation method of pumpkin polysaccharide composite hydrogel with hypoglycemic effect, and its steps are:

[0013] (1) Add pumpkin polysaccharide powder into a dry reaction kettle, then add 50% ethanol whose volume is 2-3 times the mass of pumpkin polysaccharide, and stir at 25°C at 150 rpm for 2 hours;

[0014] (2) Take NaIO whose mass is 0.45-0.50 times the mass of pumpkin polysaccharide 4 Dissolve in an appropriate amount of water to make a saturated solution, slowly add it to the reaction system, and react for 8 hours in the dark;

[0015] (3) Add ethylene glycol whose volume is 0.10-0.20 times the total mass of pumpkin polysaccharides to the reaction system, and stir at 150 rpm for 30 minutes;

[0016] (4) Add anhydrous ethanol whose volume is about 2 times that of the above reaction system to the reaction system, stir for 10 minutes, filter with suction, then dialyze with a dialysis bag with a molecular weight cut-off of 3500 for 7...

Embodiment 1

[0024] (1) Take 180g of pumpkin polysaccharide powder and add it to a dry reaction kettle, then add 540ml of 50% ethanol, and stir at 150 rpm for 2 hours at 25°C;

[0025] (2) Take 85.14g NaIO 4 Dissolve in an appropriate amount of water to make a saturated solution, slowly add it to the reaction system, and react for 8 hours in the dark;

[0026] (3) Add 22.32ml of ethylene glycol to the reaction system and stir at 150 rpm for 30 minutes;

[0027] (4) Add about 1,500ml of absolute ethanol to the reaction system, stir for 10 minutes, then suction filter, then dialyze with a dialysis bag with a molecular weight cut-off of 3,500 for 72 hours, and dry at 50°C for 12 hours to obtain oxidized pumpkin polysaccharide;

[0028] (5) Take 25g of oxidized pumpkin polysaccharide and dissolve it in 500ml of distilled water to prepare storage solution A;

[0029] (6) Weigh 50g of N,O-carboxymethyl chitosan and dissolve it in 500°C of distilled water to prepare storage solution B;

[0030...

Embodiment 2

[0033] (1) Take 180g of pumpkin polysaccharide powder and add it to a dry reaction kettle, then add 540ml of 50% ethanol, and stir at 150 rpm for 2 hours at 25°C;

[0034] (2) Take 85.14g NaIO 4 Dissolve in an appropriate amount of water to make a saturated solution, slowly add it to the reaction system, and react for 8 hours in the dark;

[0035] (3) Add 22.32ml of ethylene glycol to the reaction system and stir at 150 rpm for 30 minutes;

[0036] (4) Add about 1,500ml of absolute ethanol to the reaction system, stir for 10 minutes, then suction filter, then dialyze with a dialysis bag with a molecular weight cut-off of 3,500 for 72 hours, and dry at 50°C for 12 hours to obtain oxidized pumpkin polysaccharide;

[0037] (5) Take 25g of oxidized pumpkin polysaccharide and dissolve it in 500ml of distilled water to prepare storage solution A;

[0038] (6) Weigh 50g of N,O-carboxymethyl chitosan and dissolve it in 500°C of distilled water to prepare storage solution B;

[0039...

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Abstract

A preparation method of a pumpkin polysaccharide hydrogel composite with a hypoglycemic effect includes the steps of adding pumpkin polysaccharide powder into a dry reaction still, adding ethyl alcohol to be stirred at 25 DEG C, dissolving NaIO4 with weight accounting for 0.5-0.50 time of the weight of pumpkin polysaccharide in water to prepare a saturated solution, slowly adding the saturated solution into a reaction system for reaction in a light shielded state, adding ethylene glycol into the reaction system to be stirred, adding absolute ethyl alcohol into the reaction system to be stirred and then subjected to suction filtration, conducting dialysis for 72 hours through a dialysis bag with molecular weight cut-off of 3500, conducting drying for 12 hours at 50 DEG C to obtain oxidized pumpkin polysaccharide, weighing the oxidized pumpkin polysaccharide, dissolving the oxidized pumpkin polysaccharide in distilled water, weighing N,O-carboxymethyl chitosan with weight accounting for 2-4 times of the weight of the oxidized pumpkin polysaccharide, dissolving N,O-carboxymethyl chitosan in distilled water according to the weight volume ratio of 1:10, mixing the two solutions in equivalent volume, and standing for 10 minutes at room temperature to obtain pumpkin polysaccharide hydrogel with the hypoglycemic effect.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a method for preparing a pumpkin polysaccharide composite hydrogel with hypoglycemic effect. Background technique [0002] Due to its unique three-dimensional network structure, hydrogel has good permeability, can effectively improve drug loading, biological half-cycle and stability, etc., and has received great attention in the field of drug release. Sustained release of drugs is to combine polymer carriers and small molecule drugs by chemical or physical methods, and through various control methods in the body, so that the drugs can act on the desired concentration and release rate within a certain period of time. The location of the lesion in the body, and then achieve the purpose of reducing the number of medications and improving the utilization rate of drugs. However, the small amount of drug loaded, which cannot meet the needs of drug efficacy, is a restric...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K31/715A61K31/64A61K38/28A61K47/36A61P3/10
CPCA61K9/06A61K31/64A61K31/715A61K38/28A61K47/36A61K2300/00
Inventor 张伟杰王小春马建忠蒲秀瑛王永刚冷非凡王艳玲麻慧卢星如雷军强朵德祥郭登海
Owner 甘肃中科博瑞生物工程有限公司
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