Measuring reagent for hyaluronic acid and preparation method of measuring reagent

A hyaluronic acid and reagent technology, applied in the field of hyaluronic acid determination reagents and its preparation, can solve the problems of unfavorable large-scale detection, difficult popularization in primary hospitals, and long experiment time, so as to achieve easy automatic measurement and save waiting time , the effect of less sample consumption

Inactive Publication Date: 2016-12-07
浙江达美生物技术有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

These two methods are relatively accurate and reliable, but radioimmunoassay (RIA) has certain radioactive contamination, which is not conducive to the large-scale development of detection; Equipped with a microplate reader, it is difficult to popularize in primary hospitals

Method used

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  • Measuring reagent for hyaluronic acid and preparation method of measuring reagent
  • Measuring reagent for hyaluronic acid and preparation method of measuring reagent
  • Measuring reagent for hyaluronic acid and preparation method of measuring reagent

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Embodiment Construction

[0028] Main reagents used in this embodiment: Tris buffer 50mmol / L, hyaluronic acid binding protein 0.1mg / L, bovine serum albumin 0.1%, hyaluronan binding protein specific antibody coated latex 0.2%, bovine serum albumin 0.5% , phosphate buffer 50mmol / L, and sodium azide were all purchased from Enzymaker.

[0029] (1) Preparation of R1 reagent

[0030] ①Pour 700ml of purified water into a suitable container.

[0031] ② Turn on the electric stirrer, and the stirring speed is 450 rpm.

[0032] ③ Weigh 100g Tris and add it into the above purified water, stir until completely dissolved.

[0033] ④ Weigh 2 mg of hyaluronic acid-binding protein and add to the above solution, stir until completely dissolved.

[0034] ⑤ Take 7.2ml of bovine serum albumin and add it to the above solution, stir until completely dissolved.

[0035] ⑥Weigh 2.4g of sodium azide and add to the above solution, stir until completely dissolved.

[0036] ⑦ Stir for more than ten minutes.

[0037] ⑧Set the v...

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Abstract

The invention relates to a measuring reagent for hyaluronic acid and a preparation method of the measuring reagent, and belongs to the technical field of testing of biochemical methods. The measuring reagent comprises a reagent R1, a reagent R2 and a hyaluronic acid calibration product, wherein the reagent R1 is prepared from the following components: 50 mmol / L of a Tris buffer solution, 0.1 mg / L of a hyaluronic acid binding protein, 0.1 percent of bovine serum albumin and 0.1 percent of sodium azide; the reagent R2 is prepared from the following components: 0.2 percent of hyaluronic acid binding protein specific antibody-wrapped latex, 0.5 percent of bovine serum albumin and 0.09 percent of sodium azide; the hyaluronic acid calibration product is prepared from the following components: hyaluronic acid, 50 mmol / L of a phosphate buffer solution and 0.1 percent of sodium azide. The measuring reagent is applied to measurement of hyaluronic acid, and has the advantages of fastness, sensitivity, high accuracy, high stability, simplicity and convenience for operation, and the like.

Description

technical field [0001] The invention relates to a reagent for measuring hyaluronic acid and a preparation method thereof, belonging to the technical field of biochemical means testing. Background technique [0002] According to statistics, there are 90 million hepatitis B patients and infected persons in my country. Some patients develop from chronic hepatitis to liver cirrhosis in the course of several years or decades of chronic disease. During this process, liver fibrosis is an important stage in the progression of liver disease. , is a common pathological process of many chronic liver diseases, and is an intermediate link in the development of liver cirrhosis and even primary liver cancer. Early treatment of liver fibrosis can reverse or inhibit its development. Therefore, early detection of liver fibrosis is of great significance for timely treatment, blocking and reversing the occurrence and development of liver fibrosis, and preventing the occurrence of liver cirrhosis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68G01N2800/085
Inventor 方朝君
Owner 浙江达美生物技术有限公司
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