Wnt16 gene deletion type zebra fish

Abstract

The invention discloses a wnt16 gene deletion type zebra fish. Compared with the prior art, specific genes in the genome of a living body can be muted more efficiently and precisely; moreover, the manufacturing is simple, the cost is lower, multiple sites on target genes can be sheared at the same time, and any number of single gene can be muted. The wnt16 gene deletion type zebra fish can be applied to research related with gene and skeleton growth and other research for finding whether deletion of wnt16 gene is related with growth of other organs such as heart or not, and thus the wnt16 gene deletion type zebra fish has a good medical research value. At the same time, the growth period of zebra fishes without wnt16 gene is obviously shortened, and the wnt16 gene deletion type zebra fish also has a good commercial value therefore.

Description

technical field [0001] The invention relates to the technical field of gene knockout, in particular to a wnt16 gene-deleted zebrafish. Background technique [0002] The Wnt gene is located at human 12q13 and encodes a transcription factor of 356 amino acids. It is generally believed that the Wnt signal transduction pathway is divided into canonical Wnt signaling pathway and non-canonical Wnt signaling pathway. The canonical Wnt signaling pathway is also known as the Wnt / p-catenin signaling pathway. The molecular mechanism of Wnt / p-catenin signal transduction is highly conserved in different species. Wnt16 is a non-canonical wnt ligand. It is generally believed that this gene mediates the β-catenin signaling pathway, can negatively regulate RANK signaling, inhibit the formation of osteoclasts, and participate in processes such as bone formation and bone resorption. Through gene differential expression profile analysis and genome association analysis, it was found that Wnt1...

AI Technical Summary

Problems solved by technology

Traditional gene targeting technology is based on embryonic stem cells (ESC) and homologous recombination technology, so the efficiency of targeting technology is extremely low

At the beginning of 2013, a new artificial endonuclease clustered regularly interspacedshortpalindromic repeats (CRISPR) / CRISPR-associated (Cas) 9 can more efficiently and accurately silence specific genes in the genome of organisms, and is simple and low-cost , and can cut multiple sites on the target gene at the same time, and silence any number of single genes, but at the same time, this technology has certain defects, and its off-target rate is relatively high

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