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Chitosan derivative aquagel and preparation method thereof

A technology of chitosan derivatives and hydrogels, applied in medical science, prostheses, etc., can solve the problems of limited research and application, high toxicity, and affecting cell activity, etc., and achieve simple process, fast reaction speed, and uniform pore size distribution Effect

Active Publication Date: 2016-12-07
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Formaldehyde, glutaraldehyde and other bifunctional cross-linking agents are commonly used for chemical cross-linking, so that chitosan can be cross-linked by Schiff base to form a gel, but these cross-linking agents are highly toxic and will affect cell viability, limiting their ability to be used in cells and cells. In vivo research applications

Method used

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  • Chitosan derivative aquagel and preparation method thereof
  • Chitosan derivative aquagel and preparation method thereof
  • Chitosan derivative aquagel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) Dispersing chitosan with a viscosity-average molecular mass of 700kDa and a degree of deacetylation of 98% in a NaOH solution with a mass concentration of 50%, wherein the mass ratio of NaOH in chitosan and NaOH solution is 3:5; A solution of isopropanol in chlorohydrin was added thereto, and then the temperature was raised to 65° C. for reflux reaction for 5 hours; wherein, the number of chitosan repeating units: the molar ratio of chlorohydrin: isopropanol was 1:4.5:7.

[0031] 2) After the reaction, adjust the pH value to neutral with hydrochloric acid, wash the product several times with ethanol and absolute ethanol with a volume concentration of 85%, dissolve it in water after vacuum drying, and dialyze with a dialysis bag with a molecular weight cut-off of 3500Da After 48 hours, freeze-drying, specifically keeping at minus 20° C. for 72 hours and 20° C. for 4 hours, to obtain hydroxyethyl chitosan.

[0032] 3) preparation mass concentration is the hydroxyethyl ...

Embodiment 2

[0040] 1) The chitosan whose viscosity-average molecular weight is 300kDa and 95% degree of deacetylation is dispersed in the NaOH solution whose mass concentration is 30%, wherein the mass ratio of NaOH in chitosan and NaOH solution is 3:5; The isopropanol solution of chloroethanol was added therein, and then the temperature was raised to 65° C. for reflux reaction for 7 hours; wherein, the chitosan repeating unit: chloroethanol: isopropanol molar ratio was 1:3:5.

[0041] 2) After the reaction, adjust the pH value to neutral with hydrochloric acid, wash the product several times with ethanol and absolute ethanol with a volume concentration of 85%, dissolve it in water after vacuum drying, and dialyze with a dialysis bag with a molecular weight cut-off of 3500Da After 48 hours, freeze-drying, specifically keeping at minus 20° C. for 72 hours and 20° C. for 4 hours, to obtain hydroxyethyl chitosan.

[0042] 3) preparation mass concentration is the hydroxyethyl chitosan solutio...

Embodiment 3

[0045] 1) Dispersing chitosan with a viscosity-average molecular weight of 1000kDa and a degree of deacetylation of 91% in a NaOH solution with a mass concentration of 60%, wherein the mass ratio of NaOH in chitosan and NaOH solution is 3:5; The isopropanol solution of chloroethanol was added thereto, and then the temperature was raised to 65° C. for reflux reaction for 4 hours; wherein, the chitosan repeating unit: chloroethanol: isopropanol molar ratio was 1:4:7.

[0046] 2) After the reaction, adjust the pH value to neutral with hydrochloric acid, wash the product several times with ethanol and absolute ethanol with a volume concentration of 75%, dissolve it in water after vacuum drying, and dialyze with a dialysis bag with a molecular weight cut-off of 3500Da After 48 hours, freeze-drying, specifically keeping at minus 20° C. for 72 hours and 20° C. for 4 hours, to obtain hydroxyethyl chitosan.

[0047] 3) preparation mass concentration is the hydroxyethyl chitosan solutio...

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Abstract

The invention discloses a preparation method of a chitosan derivative aquagel, belonging to the field of biological medical materials. The preparation method of the chitosan derivative aquagel comprises the following steps: carrying out alkalification, swelling, chloroethanol modification, after-treatment and other series technical processes on chitosan to prepare hydroxyethyl chitosan, carrying out glycidyl methacrylate graft modification, and finally, carrying out photochemical polymerization to form the aquagel. The obtained aquagel has the advantages of favorable biocompatibility, low toxicity, short gelling time, controllable and adjustable crosslinking density, favorable elasticity, high structural stability and the like. The preparation method has the characteristics of accessible raw materials, simple technique, mild reaction conditions and the like. The obtained aquagel can be used in the aspects of cell delivery, tissue engineering, cell three-dimensional culture and the like.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a methacrylylated hydroxyethyl chitosan hydrogel and a preparation method thereof. Background technique [0002] Hydrogel is a polymer with a three-dimensional network structure, which is generally formed by cross-linking through covalent bonds, hydrogen bonds or van der Waals forces. It can absorb a large amount of water in water and swell without dissolving. There are many kinds of hydrogels, and there are different classification methods based on different standards. According to the source of their materials, they can be divided into natural polymer hydrogels and synthetic polymer hydrogels. Chitosan hydrogel is a typical representative of natural polymer hydrogel, which has the advantages of low toxicity, good biocompatibility and biodegradability, and has attracted extensive attention in the field of biomedical materials. Its main raw material, chitosan, is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F299/00C08F2/48C08B37/08A61L27/20A61L27/52A61L27/56
CPCA61L27/20A61L27/52A61L27/56C08B37/003C08F2/48C08F299/00C08L5/08
Inventor 钱军民张雅萍胥伟军杨明万雪丽
Owner XI AN JIAOTONG UNIV
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