Therapeutic recombinant antibody, its coding gene and application

Abstract

The invention discloses a therapeutic recombinant antibody, and an encoding gene and application thereof. In an excellent embodiment, the therapeutic recombinant antibody is mainly formed by fusion of CBD collagen specific-binding polypeptide and an Fab segment of cetuximab, and contains a light-chain variable region formed by a genetic code with a sequence shown by SEQ ID NO:1 and a heavy-chain variable region which is formed by a genetic code with a sequence shown by SEQ ID NO:2. The therapeutic recombinant antibody disclosed by the invention not only has the specific-binding capacity of collagen but also reserves the EGFR binding capacity of the cetuximab, and besides, the functions of killing tumor cells and suppressing tumor growth of the cetuximab are also maintained; and in addition, the recombinant antibody has high targeting ability and good tumor tissue infiltration capacity in bodies and can realize slow release in tumor tissues, the anti-tumor effects of the therapeutic recombinant antibody are far better than those of a total-length antibody namely the cetuximab, and the therapeutic recombinant antibody has wide application prospects in tumor detection preparations, antitumor medicines and the like.

Description

technical field [0001] The invention relates to a therapeutic antibody, in particular to a CBD-Fab fusion antibody with collagen-specific binding ability, its preparation method and its application in tumor treatment. Background technique [0002] The tumor microenvironment is composed of tumor cells, normal cells and extracellular matrix, and plays an important role in the occurrence and migration of tumors. Studies have shown that the occurrence of tumors is not only the canceration of cells, but also the microenvironment of cells and cells. Caused by dissonance between environments. Therefore, the tumor microenvironment plays an important role in the process of tumor migration. Collagen is the main component of the extracellular matrix and the main component of the tumor microenvironment. In the process of tumorigenesis, the extracellular matrix in the tumor microenvironment and the Compared with collagen, the expression of extracellular matrix in normal tissues is signi...

AI Technical Summary

Problems solved by technology

[0004] Full-length antibodies have been widely used in tumor treatment and have achieved good therapeutic effects. However, due to their large molecular weight, it is difficult to overcome the internal pressure of the tumor, and the speed of penetrating blood vessels to reach the tumor site is slow. Long antibodies circulate in the body for a long period, which is likely to cause a large immune response and obvious side effects; these factors greatly reduce the therapeutic effect of full-length antibodies

[0005] Fab (Fragment antigen binding) antibody has a molecular weight of about 50kDa and is the functional region of a full-length antibody molecule. It is composed of two chains vL-cL and vH-cH1. Due to its small molecular weight and strong penetrating power, it does not contain Fc Section, low immunogenicity, Fab antibody has been widely used in tumor therapy and targeted drug delivery, but the inhibitory effect of Fab antibody on tumor cells is still far behind that of the full-length antibody cetuximab

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