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Method for separating and extracting N-acetyl-D-glucosamine and D-glucosamine from ammonia sugar fermentation liquor

A technology of glucosamine and acetyl group, applied in amino sugar, chemical instruments and methods, sugar derivatives, etc., can solve problems such as long process route, and achieve the effects of simple operation process, few separation steps, and low production energy consumption

Active Publication Date: 2016-12-07
YANGZHOU RIXING BIO TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In terms of extracting N-acetyl-D-glucosamine products, a Chinese patent (CN105543312A) discloses a technology that mainly uses ultrafiltration to remove bacteria, activated carbon decolorization, acidification, chitosan flocculation, ion exchange, concentrated crystallization, Ethanol rinsing and other processes for extraction, the process route is longer

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  • Method for separating and extracting N-acetyl-D-glucosamine and D-glucosamine from ammonia sugar fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 10L of fermentation broth, wherein the content ratio of N-acetyl-D-glucosamine to glucosamine is 6:4, filter through a polypropylene microfiltration membrane with a pore size of 1 μm to remove impurity particles and some microbial cells in the fermentation broth, A crude extract is obtained; the operating pressure is 0.5Mpa, and the operating temperature is 30°C. The filtered crude extract is passed through a polyolefin ultrafiltration membrane with a pore size of 0.05 μm to remove residual protein, nucleic acid, and colloidal particle macromolecular impurities in the fermentation broth. The operating pressure is 0.25 MPa, the temperature is 30 ° C, and the concentration factor is 4. times. The filtrate obtained by microfiltration and ultrafiltration is then subjected to reverse osmosis to remove water or small molecular impurities. The filtrate was stirred and heated to 60°C in a water bath, and the fermentation broth was further concentrated to 2L, with a final ...

Embodiment 2

[0024]Take 50L of fermentation broth, wherein the content ratio of N-acetyl-D-glucosamine to glucosamine is 7:3, filter through a polypropylene microfiltration membrane with a pore size of 1 μm to remove impurity particles and some microbial cells in the fermentation broth, A crude extract is obtained; the operating pressure is 0.2 MPa, and the operating temperature is 30°C. The filtered crude extract is passed through a polyolefin ultrafiltration membrane with a pore size of 0.02 μm to remove residual protein, nucleic acid, and colloidal particle macromolecular impurities in the fermentation broth. The operating pressure is 0.25MPa, the temperature is 30°C, and the concentration factor is 4 times. The filtrate obtained by microfiltration and ultrafiltration is then subjected to reverse osmosis to remove water or small molecular impurities. The filtrate was stirred and heated to 70°C in a water bath, and the fermentation broth was further concentrated to 10L, so that the fina...

Embodiment 3

[0026] Take 50L of fermentation broth, wherein the content ratio of N-acetyl-D-glucosamine to glucosamine is 8:2, filter through a polypropylene microfiltration membrane with a pore size of 0.5 μm to remove impurity particles and some microbial cells in the fermentation broth , to obtain a crude extract; the operating pressure is 0.2MPa, and the operating temperature is 30°C. The filtered crude extract is passed through a polyolefin ultrafiltration membrane with a pore size of 0.02 μm to remove residual protein, nucleic acid, and colloidal particle macromolecular impurities in the fermentation broth. The operating pressure is 0.25 MPa and the temperature is 30°C. The filtrate obtained by microfiltration and ultrafiltration is then subjected to reverse osmosis to remove water or small molecular impurities. The filtrate was stirred and heated to 70°C in a water bath, and the fermentation broth was further concentrated to 10L, so that the final concentration of acetylglucosamine ...

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Abstract

The invention discloses a method for separating and extracting N-acetyl-D-glucosamine and D-glucosamine from ammonia sugar fermentation liquor. The method includes the steps of microfiltration, ultrafiltration, nanofiltration and the like, the fermentation liquor is filtered through a microfiltration membrane with the aperture of 0.1-10 micrometers to remove impurity particles and microbial thalli, protein, nucleic acid, colloidal particle large molecules and other impurities are removed through filtering by means of an ultrafiltration membrane with the aperture of 0.001-0.1 micrometer, and pigment, polypeptide, nucleotide and other small-molecular impurities are removed through a reverse osmosis system; N-acetyl-D-glucosamine is crystallized and separated from the fermentation filtrate containing N-acetyl-D-glucosamine and glucosamine. The method includes the steps of fermentation filtrate heating and concentration, seed crystal addition, temperature-control crystallization, N-acetyl-D-glucosamine crystal collection, residual glucosamine recycling and the like. The method is low in cost and does not influence the structure of N-acetyl-D-glucosamine, and the obtained N-acetyl-D-glucosamine is high in purity.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a method for separating N-acetyl-D-glucosamine from fermentation liquid by using membrane separation and crystallization technology. Background technique [0002] The full name of N-acetylglucosamine is N-acetyl-D-glucosamine (N-acetyl-D-glucosamine), and the molecular formula is C 8 h 15 NO 6 , with a molecular weight of 221.0 and a melting point of 205°C. It is a white powder and is easily soluble in water. Its structural formula is as follows: [0003] [0004] N-acetyl-D-glucosamine widely exists in nature and is the basic unit of many important polysaccharides in biological cells, especially in the exoskeleton of crustaceans (CN104293724A). N-acetyl-D-glucosamine can be used to maintain the health of bone tissue and bone joints, and can prolong the lifespan of cells. The mechanism is to improve the homeostasis of endoplasmic reticulum proteins, thereby p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H5/06C07H1/06
CPCC07H1/06C07H5/06
Inventor 史劲松龚劲松杨彪张超丁振中张和孙达锋柳志强冯小海方祥陈磊
Owner YANGZHOU RIXING BIO TECH
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