Perfluorocarbon emulsion and preparing method and application thereof

A perfluorocarbon and emulsion technology, applied in the field of biomedicine, can solve the problems of limited therapeutic efficacy, hypoxia of tumor tissue, necrosis of hypoxic area, etc., and achieve the effects of high oxygen carrying capacity, improvement of hypoxia, and improvement of therapeutic effect.

Inactive Publication Date: 2016-12-07
JE & NA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, with the rapid expansion of tumor volume, a part of the tumor tissue in the tumor is getting farther and farther away from the nearest blood vessel, which leads to insufficient tumor blood supply, which eventually leads to hypoxia in this part of the tumor tissue.
Therefore, clinically most malignant tumors have internal hypoxic areas during their growth and development, and these hypoxic areas often end up with necrosis. These reasons will further lead to clinical tumor treatment methods such as radiotherapy, The curative effect of photodynamic therapy and other treatments is limited to a certain extent

Method used

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  • Perfluorocarbon emulsion and preparing method and application thereof
  • Perfluorocarbon emulsion and preparing method and application thereof
  • Perfluorocarbon emulsion and preparing method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1: the preparation of perfluorocarbon emulsion

[0031] Weigh 40 mg of protein, dissolve in 4 mL of phosphate buffer, add 200 μL of perfluorocarbon liquid, sonicate for 5 minutes with a cell disruptor, centrifuge, remove the lower layer, and then dissolve in phosphate buffer for later use. Use a dynamic light scattering instrument to measure the particle size of perfluorocarbon particles, the results are shown in figure 1 .

[0032] figure 1 The results show that the particle size of the synthesized perfluorocarbon particles is about 200nm.

Embodiment 2

[0033] Embodiment 2: the mensuration of oxygen content variation

[0034] After the perfluorocarbon emulsion in Example 1 was filled with oxygen, it was added to deionized water, and its oxygen content was measured by an oxygen electrode. After the oxygen content is stable, apply coupling agent to the bottom of the round bottom flask and add an ultrasonic probe to detect the change value of the oxygen content under the action of ultrasound. The results are shown in figure 2 .

[0035] figure 2 The results showed that the oxygen-carrying capacity of the perfluorocarbon emulsion was significantly higher than that of the aqueous solution after filling with oxygen. Under the action of ultrasound, oxygen will have a rapid release process.

Embodiment 3

[0036] Example 3: Using Immunofluorescence Probes to Detect Changes in Oxygen Content in Tumors of Mice Under the Action of Ultrasound

[0037] The mice were divided into 5 groups with 3 mice in each group.

[0038] Mice received the following treatments:

[0039] (1) Control group: no treatment;

[0040] (2) mice only oxygen inhalation group;

[0041] (3) The mice were added to the ultrasound group at the tumor site while inhaling oxygen;

[0042] (4) mice injected with perfluorocarbon emulsion and oxygen inhalation group;

[0043] (5) Mice were injected with perfluorocarbon emulsion, oxygen was inhaled and an ultrasound group was added to the tumor site at the same time;

[0044]The sonication time was 30 minutes. After the treatment of mice in each group, 0.8 mg / hypoxic fluorescent probe was injected intraperitoneally, and the tumors of the mice were excised after 90 minutes and stained by frozen sections. Cut into 4 μm thick tumor sections. Using standard Hypoxyprob...

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Abstract

The invention relates to the technical field of biological medicine, in particular to a perfluorocarbon emulsion and a preparing method and application thereof, and particularly relates to an application to improving the oxygen content of tumors. The perfluorocarbon emulsion is prepared from perfluorocarbon and proteins, high oxygen carrying capacity is achieved, and oxygen can be rapidly released under the ultrasonic effect. An experiment indicates that the perfluorocarbon emulsion is injected into the caudal vein, a mouse is in the oxygen uptake state, the tumor site of the mouse is subjected to ultrasonic treatment, oxygen carried by the perfluorocarbon emulsion can be rapidly released under the mechanical effect of the ultrasonic treatment, and the content of oxygen at the tumor site is continuously increased. By means of the method, the oxygen-shortage condition at the tumor site can be effectively improved, and therefore the treatment effects of photodynamic therapy and radiotherapy are improved.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a perfluorocarbon emulsion and its preparation method and application, especially the application in increasing the oxygen content of tumors. Background technique [0002] Tumor refers to the neogrowth formed by the proliferation of local tissue cells in the body under the action of various tumorigenic factors, because this neogrowth is mostly in the form of space-occupying blocky protrusions, also known as neoplasm (neoplasm) . According to the cell characteristics of new organisms and the degree of harm to the body, tumors are divided into two categories: benign tumors and malignant tumors, and cancer is the general term for malignant tumors. Compared with benign tumors, malignant tumors grow faster and show invasive growth, are prone to bleeding, necrosis, ulcers, etc., and often have distant metastasis, resulting in emaciation, weakness, anemia, loss of appetite, fever, ...

Claims

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Application Information

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IPC IPC(8): A61K9/107A61K47/42A61K31/025A61P35/00A61N7/00A61M31/00
CPCA61K9/107A61K31/025A61K47/42A61M31/00A61M2202/0208A61N7/00A61M2202/0007
Inventor 刘庄宋雪娇
Owner JE & NA BIOTECH CO LTD
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