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Method for producing L-glutamine with fermentation method

A technique of glutamine and fermentation, which is applied in the field of fermentation, can solve the problems of high cost of medium, and achieve the effects of increasing the leaching rate, improving the utilization rate of strains, and increasing the amount of acid production

Active Publication Date: 2016-11-23
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the defect of high cost of fermentation medium in the prior art, and to provide a new process for preparing polyglutamic acid from crop stalks

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for fermentative production of L-glutamine, comprising the steps of:

[0022] Corynebacterium glutamicum CCTCC NO.M205028 (referring to Chinese patent 200510043704) and Brevibacterium flavum ATCC14067 (referring to "the medium selection of producing L-glutamine with Brevibacterium flavum ATCC 14067 ", amino acid and microbial resources) were cultivated respectively to a concentration of 1×10 8 Bacteria solution per mL, mixed according to the volume ratio of 2:1 to obtain the mixed bacterium solution, then inoculated into the fermentation medium according to the inoculation amount of 5% volume ratio, fermented for 34 hours, and the dissolved oxygen level was controlled at 10-15%. The L-glutamine fermented liquid was obtained; the concentration of L-glutamine in the obtained L-glutamine fermented liquid was 109 g / L.

[0023] The fermentation medium is prepared from the following raw materials in parts by weight:

[0024] Corn straw enzymatic hydrolyzate 18%, fi...

Embodiment 2

[0036] Influence of some culture medium components on acid production in Example 1 of the present invention: Table 1 shows the influence of different concentrations of fish bone meal hydrolyzate on acid production from L-glutamine. The effect of nitrogen source concentration on the production of L-glutamine is illustrated through experiments. Fish bone meal hydrolyzate not only provides nitrogen source for the growth of bacteria, but also provides the necessary for the smooth conversion of L-glutamic acid to L-glutamine. NH 4 + , but too high a concentration of NH 4 + It can inhibit the activity of glutamine synthetase, is not conducive to the conversion from L-glutamic acid to L-glutamine, and affects the extraction and refining of fermentation broth, reducing the extraction yield.

[0037] Table 1

[0038] Concentration of fish bone meal hydrolyzate %

L-Glutamine (g / L)

5

92

10

99

12

105

15

109

25

102

[0039...

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Abstract

The invention belongs to the field of amino acid fermentation and discloses a method for producing L-glutamine with a fermentation method. The method comprises the following steps: mixing seed liquid according to the volume ratio of 2 to 1 to obtain mixed bacterium liquid; then inoculating the mixed bacterium liquid into a fermentation culture medium according to the inoculation amount of 5 percent; carrying out fermentation culture for 34 hours; controlling an oxygen dissolving level to be 10 percent to 15 percent, so as to obtain L-glutamine fermentation liquid. By optimizing a fermentation culture medium formula and parameters including fermentation culture process conditions and the like, the yield of products is high and the cost is low; the method has a wide application prospect.

Description

technical field [0001] The invention relates to a method for producing L-glutamine by fermentation, which belongs to the technical field of fermentation. Background technique [0002] L-glutamine is an amino acid that is amidated by the γ-carboxyl group of L-glutamic acid. It is one of the most important conditional essential amino acids for the human body, accounting for 61% of the total free amino acids in the human body. It is the ammonia in the kidney. Carrier of nitrogen synthesized and synthesized in the liver by urea. L-glutamine is widely distributed in human skeletal muscle, blood, gastrointestinal tract, liver, kidney, and brain. It can remove ammonia and biosynthesize amino sugars, nucleic acids, and amino acids in cells. [0003] L-glutamine not only plays an important role in maintaining the metabolism, structure and function of the small intestine, promoting the growth of immune cells, preventing infection, and accelerating wound healing, but also an important...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P13/14C12R1/15C12R1/13
CPCC12P13/14C12P39/00
Inventor 唐永强王绍冰殷文娟李世奇李江雷
Owner XINJIANG FUFENG BIOTECH
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