Special complex probiotics fermented Chinese herbal medicine health-care oral solution for minks and preparation method
A technology of compound probiotics and fermented Chinese herbal medicine, which is applied in the fields of application, animal feed, animal feed, etc., can solve the problems of pollution and lack of attention to the breeding environment, and achieve the effects of mellow and refreshing taste, reduced bitter components, and improved anti-oxidation
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Embodiment 1
[0031] The raw materials for preparing the compound probiotic fermented Chinese herbal medicine health care liquid are as follows in parts by weight: 20 parts of astragalus, 20 parts of forsythia, 20 parts of isatidis, 20 parts of silver flower, 20 parts of chrysanthemum, 10 parts of lactic acid bacteria and 10 parts of bacillus.
[0032] The preparation process of compound probiotic fermented liquid is as follows:
[0033] (1) Activate and preserve each slant strain, shake flask culture respectively, use LB medium for lactic acid bacteria and bacillus, pH7-7.5, autoclave at 100-130°C for 20-40 minutes, and cultivate at 30-38°C for 12-15 minutes Hour;
[0034] (2) The primary liquid expansion culture (50L tank) of lactic acid bacteria and bacillus, the inoculum size is 10%-20%;
[0035] (3) Secondary liquid expansion culture of lactic acid bacteria and bacillus (500L tank), the inoculum size is 10%-20%;
[0036] (4) Mix the lactic acid bacteria and bacillus fermentation liqu...
Embodiment 2
[0046] The raw materials for preparing the compound probiotic fermented Chinese herbal medicine health care liquid are as follows in parts by weight: 25 parts of astragalus, 25 parts of forsythia, 25 parts of isatidis, 25 parts of silver flower, 25 parts of chrysanthemum, 15 parts of lactic acid bacteria and 15 parts of bacillus.
[0047] The preparation process of compound probiotic fermented liquid is as follows:
[0048](1) Activate and preserve each slant strain, culture them in shake flasks respectively, use LB medium for lactic acid bacteria and bacillus, pH7-7.5, autoclave at 100-130°C for 20-40 minutes, and cultivate at 30-38°C for 15 hours;
[0049] (2) The primary liquid expansion culture (50L tank) of lactic acid bacteria and bacillus, the inoculum size is 10%-20%;
[0050] (3) Secondary liquid expansion culture of lactic acid bacteria and bacillus (500L tank), the inoculum size is 10%-20%;
[0051] (4) Lactic acid bacteria and Bacillus fermentation liquid are mixe...
Embodiment 3
[0061] The raw materials for preparing the compound probiotic fermented Chinese herbal medicine health care liquid are as follows in parts by weight: 30 parts of astragalus, 30 parts of forsythia, 30 parts of isatidis, 30 parts of silver flower, 30 parts of chrysanthemum, 20 parts of lactic acid bacteria and 20 parts of bacillus.
[0062] The preparation process of compound probiotic fermented liquid is as follows:
[0063] (1) Activation and preservation of each slant strain, cultured in shake flasks respectively, LB medium for lactic acid bacteria and bacillus, pH 7-7.5, autoclaved at 100-130°C for 20-40 minutes, and cultivated at 30-38°C for 20 hours;
[0064] (2) The primary liquid expansion culture (50L tank) of lactic acid bacteria and bacillus, the inoculum size is 10%-20%;
[0065] (3) Secondary liquid expansion culture of lactic acid bacteria and bacillus (500L tank), the inoculum size is 10%-20%;
[0066] (4) Mix the lactic acid bacteria and bacillus fermentation li...
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