Total polyphenol content detection method

Abstract

A method for detecting the content of total polyphenols, belonging to the technical field of food, by making the polyphenolic compounds in the sample carry out quantitative oxidation reaction with tungstomolybdic acid in Folin's phenol reagent in an alkaline solution under dark conditions, and the tungsten Molybdic acid itself is reduced to generate a blue compound, and the depth of the blue compound is proportional to the number of phenolic groups. The absorbance of the reacted sample is measured, and the total amount of molybdenum in the sample is calculated according to the gallic acid standard curve. Phenol content. The detection method of the total polyphenol content of the present invention uses a spectrophotometer to measure the absorbance, calculates according to the standard curve of gallic acid, shortens the detection time, lowers the detection requirements, and solves the problems of high instrument requirements and complicated sample processing of other detection methods.

Description

technical field [0001] The invention belongs to the technical field of food, and in particular relates to a method for detecting the content of total polyphenols. Background technique [0002] Free radicals are harmful substances produced by oxidation reactions. It attacks almost every cell in the body. Cause human tissues and organs to be destroyed, accelerate the aging of the body and cause disease. Excessive free radicals can cause heart, brain, and vascular diseases, induce tumors, and cause senile dementia, cataracts, diabetes, liver disease, Parkinson's disease, and low immunity. Polyphenols are compounds found in plant foods that have potential health-promoting effects. Currently referred to as the "seventh group of nutrients". Since the polyphenol structure itself has multiple conjugated double bonds, even if the electrons at certain positions are taken away by free radicals, the molecular structure is stabilized by the conjugation effect to protect cells, and it...

AI Technical Summary

Problems solved by technology

[0004] According to the deficiencies in the prior art above, the technical problem to be solved by this invention is to propose a kind of total polyphenol content detection method, carry out absorbance measurement by spectrophotometer, calculate the total polyphenol content of sample according to gallic acid standard curve, shorten The detection time is reduced, the detection requirements are reduced, and the problems of high instrument requirements and cumbersome sample processing of other detection methods are solved.

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