A fully enclosed multi-target nucleic acid isothermal amplification and detection integrated device

Abstract

A fully enclosed multi-targeted nucleic acid isothermal amplification and detection integrated device, including a reaction tube shell, a main channel is opened in the middle of the reaction tube shell, and six annular shunts communicating with the main channel are evenly distributed on the bottom of the reaction tube shell There are six evenly distributed detection tube walls around the reaction tube shell. The hollow bottom of the detection tube wall communicates with the annular shunt flow channel. The top of the detection tube wall is in the form of an opening. It is sealed with the upper end of the detection tube wall, the reagent tube is put into the reaction tube shell along the main channel, the reagents required for the biological reaction are packaged in the reagent tube, the top of the reagent tube is sealed by the test tube cover, and the side or bottom of the reaction tube shell is installed There is a puncture device that punctures the reagent tube to release the reagent after the biological reaction is completed. The invention realizes the integration of amplification and detection, and has the advantages of visualization of detection results, good sealing, low false positives, rapid response, and multi-target detection.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a fully enclosed multi-target nucleic acid isothermal amplification and detection integrated device. Background technique [0002] Loop-mediated isothermal amplification technology (LAMP) is a nucleic acid amplification technology that efficiently amplifies at a constant temperature, proposed by Japanese scholar Notomi et al. in 2000. This technique requires 4 different specific primers to specifically recognize 6 specific segments of the target nucleic acid under the action of Bst DNA polymerase with strand displacement activity under the condition of 60-65°C. Loop-mediated isothermal amplification technology has higher amplification efficiency than polymerase chain reaction (PCR) and other isothermal amplification technologies, and can amplify the existing nucleic acid concentration to 10% of the original nucleic acid concentration within 60 minutes. 9 ~10 10 tim...

AI Technical Summary

Problems solved by technology

[0003] As we all know, no matter for PCR technology or isothermal amplification technology, the current nucleic acid amplification technology needs to go through three steps: nucleic acid extraction, nucleic acid amplification and result detection, and each step requires the use of corresponding instruments, a series of operations The steps are relatively cumbersome and require the operation of professionals, and the detection time is long and inconvenient

Due to the special reagents and reaction primers of loop-mediated isothermal amplification, loop-mediated isothermal amplification has higher sensitivity than other amplification techniques, so it is also more susceptible to contamination

The cross-contamination between samples and the pollution caused by laboratory aerosol make the false positive of ring-mediated isothermal amplification extremely high, and the multi-step operation increases the possibility of experimental operation contamination

At the same time, most amplification reaction tests can only target a specific target nucleic acid, and cannot be applied to the detection of multiple target nucleic acids in complex samples

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