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Treatment device and method for fermentation dregs of polymyxin sulfate

A polymyxin and treatment method technology, which is applied in the field of treatment equipment for polymyxin sulfate fermentation residue, can solve problems such as pressure on the ecosystem, achieve reduced treatment costs, high degradation capacity, and reduce bacterial resistance risk effect

Active Publication Date: 2016-11-16
内蒙古微技元生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The separated and purified fungus residue still contains some polymyxins. If it is discharged directly, it will cause huge pressure on the ecosystem. Therefore, those skilled in the art are committed to developing a degradation treatment method for polymyxins.

Method used

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  • Treatment device and method for fermentation dregs of polymyxin sulfate
  • Treatment device and method for fermentation dregs of polymyxin sulfate
  • Treatment device and method for fermentation dregs of polymyxin sulfate

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Embodiment 1

[0060] Mix the original swamp mud with the SRB enrichment medium 1:1, adjust the pH=7, and culture in the dark at a closed temperature of 35°C. Discard the supernatant every day, and supplement with fresh and equal volume of SRB enrichment medium. Co-cultivate in this way for 6 days.

[0061] SRB enriched medium is K 2 HPO 4 ·3H 2 O 0.5g / L, CaCl 2 2H 2 O 0.1g / L, MgSO 4 ·7H 2 O 2g / L, FeSO 4 ·7H 2 O 0.5g / L, NH 4 Cl 1g / L, NaCl 1g / L, sodium lactate 5g / L.

[0062] Mix the polymyxin sulfate fermentation residue and SRB sludge according to the volume of 5:1, and add water to make the solid content TS of the whole mixture system 80g / L, put them into the pretreatment tank for airtight cultivation, control pH=7, The temperature is 35° C., the stirring rate is 100 r / min, and the incubation time is 4 days to complete the pretreatment of the polymyxin sulfate fermentation residue.

[0063] The pretreated polymyxin sulfate slag was pumped into No. 1 tank, No. 2 tank, and No. 3 ta...

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Abstract

The invention discloses a treatment device and method for fermentation dregs of polymyxin sulfate. The method comprises the steps that firstly, original moor mud is provided, the original moor mud is mixed with an SRB enriched medium, a supernatant is discharged every day, fresh and equal-volume SRB enriched medium is supplemented, and the operation is conducted cyclically for cultivation for 3-10 d, so that SRB sludge is obtained; then the fermentation dregs of the polymyxin sulfate are mixed with the SRB sludge, the mixture is placed into a pretreatment tank for airtight cultivation, and the pretreated fermentation dregs of the polymyxin sulfate are obtained; and finally, the pretreated fermentation dregs of the polymyxin sulfate are pumped into an anaerobic jar for an anaerobic reaction, and the fermentation dregs of the polymyxin sulfate are degraded. The treatment method can be used for treatment for the fermentation dregs of the polymyxin sulfate, the treatment period is short, and the degrading effect is remarkable.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a treatment device and method for polymyxin sulfate fermentation scum. Background technique [0002] Polymyxin is also called colistin sulfate, English common name: Polymyxin BSulfate, molecular formula: C56H98N16O13H2SO4, molecular weight: 1301.56. Polymyxin is the general name of a family of basic polypeptide antibiotics composed of various amino acids and fatty acids, which was discovered in 1947 and produced by Bacillus polymyxa. Polymyxins produced by different strains, due to the amino acid content Different from fatty acids, they are divided into polymyxins A, B, C, D, E, and M. Polymyxin B (polymyxin B) is divided into B1 and B2, polymyxin E (polymyxin E, colistin,) is divided into E1 and E2, polymyxin M is also called polymyxin A, polymyxin Mycolistin B and polymyxin E have similar pharmacological effects and are widely used. Polymyxin B is mostly used for human me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09B3/00B09B5/00C12M1/107C12M1/02C12M1/00C12P5/02
CPCB09B3/00B09B5/00C12M21/04C12M23/58C12M27/02C12P5/023Y02E50/30
Inventor 陈代杰邵雷花逾冬
Owner 内蒙古微技元生态科技有限公司
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