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Biological adhesive based on epsilon-polylysine as well as preparation method and application thereof

A bioadhesive and polylysine technology, which is applied in plastic surgery, rehabilitation medicine, and clinical medicine, can solve the problems of poor adhesion of fibrin adhesives, tissue burns on the bonding surface, and short storage period. To achieve the effect of safe and non-toxic material source, satisfactory adhesion and repair, and good fluidity

Active Publication Date: 2016-11-16
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are many defects in these bioadhesives, such as the use of α-cyanoacrylate adhesives accompanied by local heating, which causes burns on the bonding surface tissue, and it is easy to solidify and has a short storage period, thus limiting the use of bioadhesives. Clinical use; fibrin adhesives have poor adhesive properties and there is a risk of viral infection, which also has limitations in application

Method used

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  • Biological adhesive based on epsilon-polylysine as well as preparation method and application thereof
  • Biological adhesive based on epsilon-polylysine as well as preparation method and application thereof
  • Biological adhesive based on epsilon-polylysine as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0063] Prepare 1mg / ml hyaluronic acid aqueous solution and 10mg / ml ε-polylysine aqueous solution for later use, mix the above two solutions according to the mass ratio of hyaluronic acid and ε-polylysine 1:2.5, stir evenly, After standing at 10° C. for 24 h, the above mixed solution was placed in a vacuum pump and dried in vacuum for 0.5 h to finally obtain a bioadhesive based on ε-polylysine.

[0064] The physical picture of the obtained bioadhesive figure 1 , figure 1 It is a bioadhesive based on ε-polylysine prepared in a glass bottle, and the sample is uniform and transparent without precipitation.

Embodiment 2

[0066] Prepare 50mg / ml hyaluronic acid aqueous solution and 500mg / ml ε-polylysine aqueous solution for later use, mix the above two solutions according to the mass ratio of hyaluronic acid to ε-polylysine 1:50, stir evenly, After standing at 60° C. for 0.5 h, the above mixed solution was placed in a vacuum pump and dried in vacuum for 24 h to finally obtain a bioadhesive based on ε-polylysine, which can be used to bind biological tissues.

[0067] Cut pig large intestine into 2.5cm×1cm rectangular strips, soak in PBS phosphate buffered saline to remove surface oil, and slightly dry the surface with gauze. The prepared bioadhesive was evenly applied to one end of the large intestine of the pig, and the smeared area was 1cm×1cm. The other end of the pretreated pig large intestine was covered with the applied area of ​​the bioadhesive, and it was removed after applying pressure with a weight for 30 minutes.

[0068] The bonding effect of the treated pig large intestine is as foll...

Embodiment 3

[0070] Prepare 15mg / ml hyaluronic acid aqueous solution and 100mg / ml ε-polylysine aqueous solution for later use, mix the above two solutions according to the mass ratio of hyaluronic acid to ε-polylysine 1:50, stir evenly, After standing at 50° C. for 0.5 h, add aspirin to the above mixed solution to 0.1 mM, and mix well. The above mixed solution was placed in a vacuum pump and dried in vacuum for 8 hours to finally obtain an ε-polylysine-based bioadhesive loaded with aspirin.

[0071] The rheological test of gained bioadhesive-the variation curve of viscosity with temperature is as follows image 3 , Viscosity-temperature test results show that as the temperature increases, the apparent viscosity of the ε-polylysine-based bioadhesive does not decrease significantly, indicating that this bioadhesive is a non-temperature-sensitive material. Store at room temperature, normal temperature changes will not affect the bonding performance of the adhesive.

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Abstract

The invention provides a biological adhesive based on epsilon-polylysine as well as a preparation method and application thereof. The adhesive is prepared from epsilon-polylysine, hyaluronic acid and water and is jointly assembled by the epsilon-polylysine and the hyaluronic acid. The biological adhesive provided by the invention has the advantages of safe and toxin-free raw materials, good biocompatibility and capability of being degraded along time; the biological adhesive can be used for replacement of suture lines of medical surgical operations and plastic and aesthetic surgeries; the product can be absorbed by body issues and does not generate toxic side effect; meanwhile, the product has proper elasticity and toughness and meets the requirements on mechanical properties by biological adhesion.

Description

technical field [0001] The invention belongs to the technical fields of clinical medicine, rehabilitation medicine and plastic surgery, and in particular relates to a bioadhesive based on ε-polylysine and its preparation method and application. Background technique [0002] Traditional surgical operations, plastic surgery, etc. cannot get rid of the use of instruments and sutures. However, the suture method is difficult and time-consuming to operate, and it brings a lot of inconvenience to remove the stitches and change the dressing. The wound is prone to infection and suppuration, and local hyperplasia occurs. . Traditional treatment methods can no longer meet the requirements of modern medicine, and new biomedical materials—bioadhesives have attracted more and more attention because of their excellent fluidity, adhesive properties, effective local release of drugs, and excellent cosmetic effects. At present, the development of bioadhesives in the medical field is limited ...

Claims

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Application Information

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IPC IPC(8): A61L24/08A61L24/04
Inventor 闫学海邢蕊蕊任朋邹千里
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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