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Ultrasonic standing wave type microfluidic chip and manufacturing method thereof

A microfluidic chip and ultrasonic standing wave technology, applied in the field of microfluidics, can solve the problems of manipulating short nanofiber wires, achieve good shape, good effect, and have the effect of ultrasonic standing wave response characteristics

Inactive Publication Date: 2016-11-09
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Manipulation of short and dispersed nanofiber wires in ultrasonic standing wave microfluidic chips has not been reported yet

Method used

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  • Ultrasonic standing wave type microfluidic chip and manufacturing method thereof
  • Ultrasonic standing wave type microfluidic chip and manufacturing method thereof
  • Ultrasonic standing wave type microfluidic chip and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The specific process of preparation and functional modification of PLGA microspheres is as follows:

[0054] (1) Weigh 100mg PLGA and dissolve in 2mLCH 2 Cl 2 200μL of ultrapure water as the inner water phase, mix the organic phase and the inner water phase, and ultrasonically treat for 30s in an ice-water bath to form a W / O emulsion; transfer the above W / O emulsion into 50mL of 5% PVA aqueous solution In the state of ice-water bath, use a homogenizer (6000rpm, 5min) to make the droplets form a W / O / W emulsion with a relatively uniform size; drop the obtained W / O / W emulsion into 80mL of 2% isopropanol aqueous solution , under the condition of 39°C water bath, after magnetic stirring for 4h, centrifuge (4000rpm, 3min), discard the supernatant, wash the centrifuged residue with 30mL ultrapure water, repeat the above centrifugal washing operation three times until the supernatant is clear and colorless, After centrifugation, the centrifuged residue was redispersed in 30ml...

Embodiment 2

[0059] Ultrasonic standing wave microfluidic chip such as Figure 12 As shown, the specific steps are as follows:

[0060] (1) at 76×26mm 2 On the glass slide, fix the rectangular capillary (length is 50mm, width is 0.2mm, height is 2mm) with epoxy resin glue, this rectangular capillary is used as the channel of microfluidic chip;

[0061] (2) At the outlets on both sides of the rectangular capillary, a polyethylene tube with an inner diameter of 1 mm and an outer diameter of 1.4 mm is used as the input port and the output port of the suspension;

[0062] (3) Finally, on the side of the fixed rectangular capillary, fix the piezoelectric ceramic sheet (15mm×2mm×0.2mm) by epoxy resin bonding, and then weld the wires on the front and back of the piezoelectric ceramic sheet as the circuit connection wire .

[0063] Take 2mL of the aqueous solution (0.2mg / mL) of the product obtained in (1) in Example 1 and place it in a 5mLEP tube as a liquid storage tube. Before the test, the m...

Embodiment 3

[0065] Take 500 μL (1 mg) of the PLGA-PEI aqueous solution of the product obtained in (2) in Example 1, dilute it to 1 mL (concentration is 0.5 mg / mL) with a serum-free medium, place it in a 5 mLEP tube, and add the serum-free solution that has been digested and centrifuged. HeLa cells (1 million) suspended in the culture medium, with a total volume of 2 mL, were co-cultured at 37° C. for 30 min to obtain a solution of cell-microsphere conjugates. Before the test, the microfluidic chip was soaked in 2% BSA for 2 hours, and the ultrasonic standing wave microfluidic device platform was built. First, it was tested whether the voltage was applied to both ends of the piezoelectric ceramic, and the output signal of the signal generator was adjusted to a voltage of 20Vpp. , the frequency is 10KHz, the standard is that you can hear a buzzing sound after adding this signal, otherwise there will be no signal at both ends of the piezoelectric ceramic, then you need to check whether the co...

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Abstract

The invention relates to an ultrasonic standing wave type microfluidic chip and a manufacturing method thereof. According to the chip, particles or cells are controlled, captured and separated in ultrasonic standing waves by PLGA-PEI microspheres or PLGA short nanofiber lines in a chip channel. The method comprises the steps that 1, the PLGA microspheres are synthesized through a double emulsion method and then modified by polyethyleneimine; 2, a PLGA nanofiber membrane is prepared through an electrostatic spinning method and then homogenized to obtain the short nanofiber lines; 3, the ultrasonic standing wave type microfluidic chip is manufactured. According to the chip, the particles or cells can be effectively controlled, the manufacturing process is simple, cost is low, the very high controllability and operability are achieved, and a wide application prospect is achieved.

Description

technical field [0001] The invention belongs to the field of microfluidic technology, in particular to an ultrasonic standing wave microfluidic chip and a preparation method thereof. Background technique [0002] Microfluidics is one of the most cutting-edge technologies in the world. It is a highly interdisciplinary technology that integrates melting, physics, life science, microelectronics, materials, and computers. It is currently mainly used in the fields of chemistry and life science. It is a technique for manipulating or controlling nanoliter or picoliter-level liquids in micron-scale channels, and the entire analysis process from sampling, sample pretreatment, reaction, separation and enrichment to detection is on an integrated microchip Complete, while greatly reducing the amount of samples and reagents, greatly improve the analysis speed, realize the miniaturization, integration and automation of the analysis system, and have the advantages of low cost, high throug...

Claims

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Application Information

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IPC IPC(8): B01L3/00
CPCB01L3/5027B01L3/502707B01L2200/0647B01L2200/12B01L2300/12B01L2300/163B01L2400/0439
Inventor 朱晓玥尹迪魏延传徐刚伟王梦媛史向阳
Owner DONGHUA UNIV
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