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Extraction method for dissolved-regenerated lignin

An extraction method and lignin technology, applied in lignin derivatives, chemical instruments and methods, organic chemistry, etc., can solve the problems of difficulty, complex lignin extraction process, and low yield

Inactive Publication Date: 2016-11-02
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using LiCl / DMSO mixed solution to dissolve lignocellulosic raw materials and then regenerate and extract lignin mainly solves the problems of complex lignin extraction process, high difficulty, low yield and difficult control of extraction conditions in existing methods.

Method used

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  • Extraction method for dissolved-regenerated lignin
  • Extraction method for dissolved-regenerated lignin
  • Extraction method for dissolved-regenerated lignin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Take the stalk part of the soybean stalk, pulverize it with a miniature plant grinder, and then process it with a planetary ball mill for 0.5 h to obtain lignocellulose powder;

[0033] (2) Weigh 5g of powder processed by ball mill for 0.5h, then dissolve it in 8% LiCl / DMSO lignocellulose total solution system with 5% concentration, and after magnetic stirring at room temperature for 24h, amber clear and translucent lignocellulose / LiCl / DMSO solution;

[0034] (3) Add the lignocellulose / LiCl / DMSO solution dropwise to 5 times the volume of distilled water, stir and disperse evenly, let it stand for a while, centrifuge the regenerated lignocellulose product and wash it with distilled water until AgNO 3 Check for Cl - exist;

[0035] (4) After washing the lignocellulose dissolving-regenerated product with distilled water, directly add it in the acetic acid-sodium acetate buffer solution with a pH value of 4.8, and then add 400FPU Commercial enzymes;

[0036] (5) R...

Embodiment 2

[0041] (1) Take the stalk part of the soybean stalk, pulverize it with a miniature plant grinder, and then process it with a planetary ball mill for 1.0 h to obtain lignocellulose powder;

[0042] (2) Weigh 5g of powder processed by ball mill for 1.0h, then dissolve it in 8%LiCl / DMSO wood fiber total solution system with 5% concentration, and after magnetic stirring at room temperature for 24h, amber clear and translucent lignocellulose / LiCl / DMSO solution;

[0043] (3) Add the lignocellulose / LiCl / DMSO solution dropwise to 5 times the volume of distilled water, stir and disperse evenly, let it stand for a while, centrifuge the regenerated lignocellulose product and wash it with distilled water until AgNO 3 Check for Cl - exist;

[0044] (4) After washing the lignocellulose dissolving-regenerated product with distilled water, directly add it in the acetic acid-sodium acetate buffer solution with a pH value of 4.8, and then add 400FPU commercial enzymes,

[0045] (5) Regula...

Embodiment 3

[0050] (1) Take the stalk part of the soybean stalk, pulverize it with a miniature plant grinder, and then process it with a planetary ball mill for 2.0 hours to obtain lignocellulose powder;

[0051] (2) Weigh 5g of powder processed by ball mill for 2.0h, then dissolve it in 8%LiCl / DMSO lignocellulose total solution system with 5% concentration, and after magnetic stirring at room temperature for 24h, amber clear and translucent lignocellulose / LiCl / DMSO solution;

[0052] (3) Add the lignocellulose / LiCl / DMSO solution dropwise to 5 times the volume of distilled water, stir and disperse evenly, let it stand for a while, centrifuge the regenerated lignocellulose product and wash it with distilled water until AgNO 3 Check for Cl - exist;

[0053] (4) After washing the lignocellulose dissolving-regenerated product with distilled water, directly add it in the acetic acid-sodium acetate buffer solution with a pH value of 4.8, and then add 400FPU commercial enzymes,

[0054] (5...

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Abstract

The invention relates to an extraction method for dissolved-regenerated lignin. The method consists of: crushing lignocellulose and conducting ball milling treatment, then dissolving the ball milled lignocelluloses powder in a 8% LiCl / DMSO mixed solution, carrying out magnetic stirring at room temperature for 24h to obtain a lignocellulose solution, adding the lignocellulose solution into certain amount of distilled water, performing stirring to disperse it uniformly, conducting centrifugal separation on regenerated lignocellulose and performing washing with distilled water, hydrolyzing the regenerated lignocelluloses in enzyme, then carrying out centrifugal separation on the regenerated supernatant and insoluble enzymolysis residue, washing the insoluble residue with a buffer solution, and then performing washing with deionized water so as to obtain enzyme hydrolysis residue, using a dioxane aqueous solution to extract the enzyme hydrolysis residue, then performing rotary evaporation to remove the dioxane aqueous solution therein to obtain crude lignin, and dissolving the crude lignin in acetic acid, then adding the mixture into a large amount of distilled water to precipitate and regenerate lignin, thus obtaining the wood fiber dissolved-regenerated lignin. The method provided by the invention has a simple process and can extract lignin efficiently.

Description

technical field [0001] The invention belongs to the technical field of biomass utilization, and mainly relates to a method for extracting dissolved-regenerated lignin. Background technique [0002] Lignocellulosic raw materials mainly come from agricultural and forestry wastes, waste paper and energy crops, and are mainly composed of three major parts: cellulose, hemicellulose and lignin. How to efficiently utilize these renewable resources has become a hot spot for scholars. In order to select better biomass resources or optimize the biomass conversion process, it is necessary to conduct detailed studies on its chemical composition and the chemical structure of each component in the cell wall. As a filler, lignin forms a complex cell wall structure with cellulose and hemicellulose, which can resist external mechanical damage and microbial invasion. [0003] The research on the structure and biosynthesis of lignin can be traced back a century ago, but there is still no def...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H7/00C07G1/00
CPCC07G1/00
Inventor 刘祝兰曹云峰皮成忠孟令坤陈俊青
Owner NANJING FORESTRY UNIV
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