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Stopped-flow two-dimensional liquid chromatography allowing two-dimensional analysis to be simultaneously carried out and application thereof

A two-dimensional liquid chromatography and liquid chromatography technology, which is applied to the analysis of materials, material separation, and measurement devices, can solve problems such as excessive sample loading, invisible transfer volume of one-dimensional effluent components, and large pressure pulses. , to improve the accuracy and repeatability, improve the pressure maintenance effect, and avoid the effect of pressure change

Active Publication Date: 2016-10-26
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0007] In order to overcome the problems of large pressure pulse, invisible one-dimensional effluent components and excessive transfer volume in the existing stopped-flow liquid chromatography, the primary purpose of the present invention is to provide a stopped-flow liquid chromatography that performs two-dimensional analysis simultaneously. Two-dimensional liquid chromatography, the chromatographic system uses a multi-port valve to close both ends of the first-dimensional chromatographic column to achieve stop flow, which greatly improves the pressure maintenance effect, and the interference of pressure fluctuations is smaller. The chromatographic column with the same column pressure drop acts as a pressure balance column, so that the first-dimensional infusion pump can still continue to flow after the first-dimensional chromatographic column is stopped, so that the first-dimensional detector can be connected to the effluent components of the first-dimensional chromatographic column. detection
In addition, before the injection of the second-dimensional liquid chromatography, the post-column splitter is used to split the flow, thus solving the problem of excessive sample loading

Method used

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  • Stopped-flow two-dimensional liquid chromatography allowing two-dimensional analysis to be simultaneously carried out and application thereof
  • Stopped-flow two-dimensional liquid chromatography allowing two-dimensional analysis to be simultaneously carried out and application thereof
  • Stopped-flow two-dimensional liquid chromatography allowing two-dimensional analysis to be simultaneously carried out and application thereof

Examples

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Embodiment 1

[0050] A stopped-flow two-dimensional liquid chromatography, such as Figure 1-2 As shown, it includes a first-dimension infusion part, a separation switching device, a first-dimension detector 1 and a second-dimension separation detection part;

[0051] The separation switching device consists of a stop-flow switching valve c (five-slot, two-position electric ten-port valve), a sample injection switching valve g (six-slot, two-position electric twelve-port valve), two post-column splitters (respectively variable speed splitter m and sample splitter f).

[0052] When in the sampling state (such as figure 1 ), the odd-numbered position and the even-numbered position of the stop-flow switching valve c and the sampling switching valve g are communicated in pairs through the diversion groove, and the first-dimensional liquid chromatography pump a pumps the mobile phase to the injector b, and the The sample and the mobile phase pass through the No. 1 and No. 2 positions of the st...

Embodiment 2

[0057] A stopped-flow two-dimensional liquid chromatography, such as Figure 3-4 As shown, the difference from Example 1 is that in the separation switching device, the stop-flow switching valve c is a four-slot two-position electric eight-way valve, the sample injection switching valve g is a five-slot two-position electric ten-way valve, and the variable speed splitter m and the one-dimensional detector l are removed (the dotted line in the figure), and in addition, the second liquid chromatography pump i samples the single pump. Other than that, its structure and working principle are consistent with Embodiment 1.

[0058] In order to further prove the innovation of the present invention, the embodiment of the present invention uses a C18 column as the first-dimensional liquid chromatography column, and uses a gel chromatography column that cannot be used as the second-dimensional liquid chromatography column of the traditional two-dimensional chromatography system as the s...

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Abstract

The invention discloses stopped-flow two-dimensional liquid chromatography allowing two-dimensional analysis to be simultaneously carried out and application thereof. Flow stopping of a chromatographic system is achieved in the mode that multi-way valves are used for sealing the two ends of a first-dimensional chromatographic column, the pressure maintenance effect is greatly improved, and pressure fluctuation interference is smaller; the other chromatographic column with the same pressure drop as the first-dimensional chromatographic column is adopted as a pressure balance column, so that a first-dimensional infusion pump does not stop flowing after flow stopping of the first-dimensional chromatographic column, and then a first-dimensional detector can be connected to detect outflow components of the first-dimensional chromatographic column. In addition, before sample injection of the second-dimensional liquid chromatographic column, a post-column flow divider is adopted for flow dividing, and the problem of excessive sample injection is solved.

Description

technical field [0001] The invention belongs to the field of instrument analysis, and in particular relates to a stopped-flow two-dimensional liquid chromatography, in particular to a stopped-flow two-dimensional liquid chromatography for simultaneous two-dimensional analysis and an application thereof. Background technique [0002] As an emerging chromatographic separation technology, two-dimensional liquid chromatography has a much higher separation efficiency than traditional one-dimensional liquid chromatography, so it has a very high application prospect in the separation and analysis of complex components. However, due to the high price of commercial two-dimensional liquid chromatography, the fixed working mode of chromatography and the poor applicability of the instrument greatly limit its development. In addition, since two-dimensional liquid chromatography is developed on the basis of one-dimensional liquid chromatography, the structures of the first-dimensional and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/46
CPCG01N30/02G01N30/461G01N2030/027
Inventor 赵谋明徐巨才苏国万赵容钟
Owner SOUTH CHINA UNIV OF TECH
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