Method for artificially synthesizing novel anti-clubroot brassica napus material

Abstract

The invention discloses a method for artificially synthesizing a novel anti-clubroot brassica napus material. The method includes: taking anti-clubroot brassica oleracea as a female parent to hybridize with anti-clubroot pakchoi; after 15 days of pollination of a generation F1, selecting seeds for embryo rescue, using a B5 fluid medium containing 2% of sucrose to culture immature seeds, and after immature embryos emerge and send up cotyledons, transferring the embryos to the B5 fluid medium containing 2% of sucrose, using 0.1% colchicines in 3-4-leaf stage to treat for 2h to realize doubling germination, cleaning, transferring into soil, and enabling selfing of synthesized plants by means of artificial pollination or saltwater spraying in a flowering stage; in a germination stage of selfing seeds, adopting a clubroot P4 physiological race for inoculation while taking an infected material as a contrast, inoculating at the same time, taking out seedling roots after 35 days, cleaning, observing whether lumps exist or not, and determining that the synthesized plants are resistant to clubroot if no lump exists. An effective approach is provided for breeding of anti-clubroot brassica napus strains, and clubroot inoculation efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of artificial synthesis of Brassica napus, in particular to a method for artificially synthesizing a new material of Brassica napus resistant to clubroot. Background technique [0002] Clubroot is a fungal disease caused by Plasmodiophora brassicae, which is distributed all over the world, especially in temperate regions. In 1995, rapeseed clubroot was reported for the first time in my country, and then the disease spread across the country on a large scale. At present, it is distributed in most provinces, municipalities, and autonomous regions, but it is mainly distributed in Yunnan, Sichuan, Guizhou, Hubei, Anhui, southern Shaanxi, etc. land. The pathogen infects and harms almost all cruciferous plants, especially Brassica plants, such as Chinese cabbage, cabbage, etc. (Piao et al., 2009). Clubroot occurs from the seedling stage, mainly infecting the roots, causing slow growth of plants, yellowing of leav...

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Problems solved by technology

[0005] At present, the inoculation methods for clubroot disease in most places are relatively extensive, and even many units only use the results of field inoculation identification as a judgment whether the plants are resistant to clubroot disease, and even some inoculations in the laboratory are mostly inoculated with bacterial solution mixed with soil method, it often happens that individuals are not successfully inoculated, which brings great errors to the identification of clubroot resistance of germplasm resources, often identified as clubroot resistance materials in the previous year, but in the next year they show clubroot resistance sick

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