Nano titanium dioxide loaded l-serine imprinted polymer thin layer plate and its preparation method

Abstract

The invention provides a nanometer titanium dioxide-loaded L-serine imprinted polymer thin layer plate and a preparation method thereof. According to the invention, nanometer titanium dioxide is used as a carrier, L-serine is used as a template, and an L-serine molecularly imprinted polymer is prepared by using a surface imprinting method; the surface of titanium dioxide is activated by using a nitric acid solution, then gamma-MAPS is used for modifying activated titanium dioxide, so functional groups on the surface of a solid is improved, and the carrier has improved capability in surface grafting with the L-serine molecularly imprinted polymer; and then the thin layer plate is prepared from the obtained molecularly imprinted polymer, n-butanol-glacial acetic acid-water (V / V, 4: 1: 1) of ninhydrin with a concentration of 0.1% is used as a developing solvent, and when the developing solvent is used for separation of chiral compounds L-serine and D-serine, a relative Rf value is greater than 2.0, so L-serine and D-serine are separated. Thus, the problem that conventional thin-layer chromatography and conventional liquid chromatography cannot realize separation of a stationary phase is overcome, and separation of a molecularly imprinted thin layer of chiral amino acid compounds is realized. The invention provides a novel material and method for chiral resolution and compartment analysis of serine.

Description

Technical field [0001] The invention relates to a thin-layer plate loaded with L-serine imprinted polymer on the surface of nano titanium dioxide and a preparation method thereof. Background technique [0002] Molecularly imprinted polymer (MIP) is a polymer synthesized by molecular imprinting technology that specifically recognizes and selectively adsorbs specific target molecules (template molecules) and their structural analogs. MIP preparation principle: mix imprinted molecules (template molecules) with suitable functional monomers, and polymerize them with a cross-linking agent to form a three-dimensional cross-linked polymer network with a high degree of cross-linking around the imprinted molecules, and then Then use an appropriate method to remove the imprinted molecules, and the final polymer is a molecularly imprinted polymer. MIP has the properties of recognition, structure-effect pre-determination, practicability, etc., so it has broad application prospects in many fi...

AI Technical Summary

Problems solved by technology

Conventional liquid chromatography stationary phase and thin layer chromatography are unable to achieve resolution of serine chiral compounds