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Injectable self-repairing hydrogel based on polysaccharides, preparation method and application of hydrogel to biological tissue engineering

A self-healing and hydrogel technology, applied in the application field of biological tissue engineering, can solve the problems of reduced service life of materials, achieve increased stability, simple and controllable synthesis method, and improve application prospects

Active Publication Date: 2016-10-12
深圳智凝高分子材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the self-healing function is one of the most remarkable features of organisms, but in synthetic materials, due to the degradation of microorganisms in the external environment, or when the external strain and stress are damaged, the service life of the material will mostly be reduced.

Method used

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  • Injectable self-repairing hydrogel based on polysaccharides, preparation method and application of hydrogel to biological tissue engineering
  • Injectable self-repairing hydrogel based on polysaccharides, preparation method and application of hydrogel to biological tissue engineering
  • Injectable self-repairing hydrogel based on polysaccharides, preparation method and application of hydrogel to biological tissue engineering

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Experimental program
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Embodiment 1

[0022] 1) 2.05g chitosan was stirred and dissolved in 100mL distilled water at room temperature. 1.5 mL of acrylic acid was slowly added dropwise, the temperature was raised to 50°C, and the reaction was carried out under magnetic stirring for 3 days to obtain a light yellow clear viscous liquid. The obtained polymer solution was cooled to room temperature, and 1M sodium hydroxide was added to adjust the pH of the system to 12. Dialyze with a 14,000 molecular weight dialysis bag for 3 days with constant water changes. The dialyzed polymer solution was used to remove most of the water with a rotary evaporator to obtain a clear liquid with a certain viscosity. Freeze-dry at -50°C for 3 days to obtain fluffy and porous N-carboxymethyl chitosan (N-carboxymethyl chitosan) solid powder A with a yield of about 75%.

[0023] 2) Take 1.00 g of sodium hyaluronate, stir and dissolve it in 100 mL of distilled water at room temperature, add sodium periodate (NaIO 4 ) 0.5528g, and stirre...

Embodiment 2

[0027] 1) 2.05g ethylene glycol chitosan was stirred and dissolved in 100mL distilled water at room temperature. 1.5 mL of acrylic acid was slowly added dropwise, the temperature was raised to 50°C, and the reaction was carried out under magnetic stirring for 3 days to obtain a light yellow clear viscous liquid. The obtained polymer solution was cooled to room temperature, and 1M sodium hydroxide was added to adjust the pH of the system to 12. Dialyze with a 8000 molecular weight dialysis bag for 3 days with constant water changes. The dialyzed polymer solution was used to remove most of the water with a rotary evaporator to obtain a clear liquid with a certain viscosity. Freeze-dry at -50°C for 3 days to obtain fluffy and porous N-carboxymethylethylene glycol chitosan solid powder A with a yield of about 75%.

[0028] 2) Take dextran sulfate 1.00g, stir and dissolve in 100mL distilled water at room temperature, add sodium periodate (NaIO 4 ) 0.30 g, and stirred for 4 hours...

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Abstract

The invention discloses injectable self-repairing hydrogel based on polysaccharides, a preparation method and application of the hydrogel to biological tissue engineering, and belongs to the technical field of high polymer materials. The method includes the steps of modifying natural high polymer chitosan so that the defect that the chitosan can only be dissolved in acid solutions can be overcome and the dissolubility of the chitosan in water can be improved; conducting mixing reaction on the chitosan and a modified sodium hyaluronate derivative solution, delaying the reaction time or raising the temperature so that sol can be converted into gel and hydrogel can be endowed with the injection characteristic and the self-repairing characteristic. It is shown through cell co-culture experiments that the intelligent hydrogel has the advantage of high biocompatibility, degradability and the like. The injectable self-repairing hydrogel as a novel medical high polymer carrier material has potential biological and medical application value when used for the co-culture together with cells, protein, DNA, antigens and other active substances, and even the delivery, release and other aspects of medicine and other in-vitro substances.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and in particular relates to a polysaccharide-based injectable self-repairing hydrogel, a preparation method and its application in biological tissue engineering. Background technique [0002] In recent years, the research work on smart materials has been unprecedentedly active, among which the development of new soft matter materials with smart phase change behavior is the fastest. Such materials can sense changes in surrounding environmental conditions, such as temperature, acid-base, pressure, sound waves, electric fields, magnetic fields, and light waves, and produce corresponding phase changes at the same time. Hydrogels are one of them. It is a three-dimensional network structure composed of two types of groups, hydrophilic and hydrophobic. The hydrophilic part absorbs a large amount of water, giving the hydrogel fluidity similar to liquid; at the same time, the hydrophobic part ...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L71/02C08L5/08C08L5/02C08B37/08C08B37/02C08G65/332A61L27/26A61L27/52
CPCA61L27/26A61L27/52C08B37/0006C08B37/003C08B37/0072C08G65/3322C08G2650/04C08J3/075C08J2371/02C08J2405/02C08J2405/08C08L71/02C08L2201/06C08L2203/02C08L2205/02C08L2205/025C08L2205/03C08L2312/06C08L5/08C08L5/04C08L5/02
Inventor 林权赵月杨旭东王威姜英男张川杨柏
Owner 深圳智凝高分子材料科技有限公司
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