Nano-drug carrier with magnetothermal and photothermal effects and preparation method thereof
A nano-drug carrier and photothermal effect technology, applied in the field of materials science, can solve the problems of inability to obtain therapeutic effects, and achieve good magnetocaloric effects and good encapsulation effects
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Embodiment 1
[0034] The nano-drug carrier provided in this example includes mesoporous silica particles, Fe embedded in the mesoporous silica particles 3 o 4 Nanoparticles and graphene oxide coated on the surface of mesoporous silica particles. The nano drug carrier preparation method comprises the following steps:
[0035] Step 1, 1.5 mmoles of FeCl 3 ·6H 2 O was dissolved in 20 ml of ethylene glycol (EG), and then 7.5 mmol ethylenediamine (EDA) and 0.75 mmol sodium citrate (NaCit) were added to the above solution and sonicated until completely dissolved to obtain a clear solution; then, the clear The solution was transferred to a hydrothermal kettle (polytetrafluoroethylene lined, stainless steel kettle body), at 220 o Cool at room temperature after solvent heat treatment at C for 10 hours; the black precipitate is collected by high-speed centrifugation, washed with ethanol, and dried in vacuum to obtain Fe 3 o 4 nanoparticles.
[0036] Step 2, 0.2 g of Fe 3 o 4 The nanoparticle...
Embodiment 2
[0044] Take 50 mg of the amino-functionalized Fe prepared in Example 1 3 o 4 / mSiO 2 Mesoporous nanoparticles, dispersed in 10 ml of PBS buffer solution of doxorubicin drug (adriamycin concentration 0.5 mg / ml), the above mixture was shaken at room temperature for 24 hours in a light-proof shaker, then centrifuged and washed with PBS buffer , and finally get Fe for storage of doxorubicin drug 3 o 4 / mSiO 2 Mesoporous nanoparticles.
[0045] Add 650 microliters of EDC and 130 microliters of NHS at 2.8 mmol / liter to 20 milliliters of graphene oxide aqueous solution (1 mg / ml) for activation treatment for 1 hour; 3 o 4 / mSiO 2 The mesoporous nanoparticles were added to the activated graphene oxide solution and reacted in the dark for 12 hours; the reddish-brown colloidal particles were collected by high-speed centrifugation, washed with deionized water and freeze-dried to obtain the graphene oxide-coated Fe for doxorubicin storage. 3 o 4 / mSiO 2 mesoporous nanoparticles, ...
Embodiment 3
[0051] Figure 4 The results of cytotoxicity of the nano-drug carriers with magnetocaloric and photothermal effects prepared in Example 1.
[0052] In vitro cytotoxicity was determined using the standard Cell Counting Kit-8 method. Breast cancer cell line 4T1 cell line was purchased from Shanghai Institute of Biochemical Cells and cultured according to the provided method.
[0053]The specific experimental process is as follows: the nano-drug carrier prepared in Example 1 was dispersed in DMEM culture medium and formulated to a concentration of 1 mg / ml. After 4T1 cells were seeded in a 96-well plate (cell density 5000 cells / well), the nano-drug The carrier suspension was immediately added to the 96-well plate, and the final nano drug carrier concentrations were 25, 50, 75, 100 and 200 μg / ml, respectively, and the solution volume was 100 μl.
[0054] After the nano-drug carrier suspension was co-cultured with the cells for 24 hours, 10 microliters of CCK-8 solution was added ...
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