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Biological tissue mass spectrometry imaging method

A mass spectrometry imaging and biological tissue technology, applied in the field of mass spectrometry imaging, can solve problems such as unfavorable optimization of mass spectrometry imaging images, inability to adjust mass spectrometer signals, complex sample processing, etc., and achieve the effects of wide application prospects, high sensitivity, and accurate detection results

Active Publication Date: 2016-09-28
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Traditional mass spectrometry imaging has the following disadvantages: (1) It is slow and cannot be directly imaged; (2) The matrix interference is serious and the sample pretreatment is complicated; (3) The ionization imaging technology after laser analysis cannot adjust the mass spectrometry signal in time and space , which is not conducive to optimizing the mass spectrometry image

Method used

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Experimental program
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Effect test

Embodiment 1

[0027] The biological tissue mass spectrometry imaging method provided in this embodiment includes the following steps:

[0028] (1) Select the isolated mouse kidney tissue, freeze and slice it, and obtain the mouse kidney tissue section;

[0029] (2) Select a corundum rod, attach the mouse kidney tissue section obtained in step (1) to the corundum rod, and let it dry naturally;

[0030] (3) Add the acriflavine solution dropwise on the mouse kidney tissue section, and let it stand to make it spread evenly;

[0031] (4) Fix the corundum rod with the mouse kidney tissue slices on the injection rod of the mass spectrometer, enter the mass spectrometer with laser through the injection system for detection, and obtain the acriflavine in the mouse kidney tissue mass spectrum.

[0032] In the step (1), the mice are selected as Balb / c male mice, 7-8 weeks old.

[0033] The concentration of acriflavine solution in step (3) is 10 -3 g / mL, 10 -3 When the acriflavine solution of g / mL...

Embodiment 2

[0041] The biological tissue mass spectrometry imaging method provided in this embodiment includes the following steps:

[0042] (1) Select the isolated mouse kidney tissue, freeze and slice it, and obtain the mouse kidney tissue section;

[0043] (2) Select a corundum rod, attach the mouse kidney tissue section obtained in step (1) to the corundum rod, and let it dry naturally;

[0044] (3) Fix the corundum rod with the mouse kidney tissue slices on the injection rod of the mass spectrometer, enter the mass spectrometer with laser through the sample injection system for detection, and obtain the acriflavine in the mouse kidney tissue mass spectrum.

[0045] In the step (1), the mice are selected as Balb / c male mice, 7-8 weeks old.

[0046]In step (3), the corundum rod adhered to the mouse kidney tissue slice is fixed on the sample rod of the mass spectrometer, and then enters the mass spectrometer with laser for detection through the sample feeding system. The specific proc...

Embodiment 3

[0049] The biological tissue mass spectrometry imaging method provided in this embodiment includes the following steps:

[0050] (1) Select the isolated mouse kidney tissue, freeze and slice it, and obtain the mouse kidney tissue section;

[0051] (2) Select a corundum rod, attach the mouse kidney tissue section obtained in step (1) to the corundum rod, and let it dry naturally;

[0052] (3) 10 μL of 10 -3 g / mL acriflavine solution, let it stand to make it diffuse evenly;

[0053] (4) Fix the corundum rod with the mouse kidney tissue slices on the injection rod of the mass spectrometer, enter the mass spectrometer with laser through the injection system for detection, and obtain the acriflavine in the mouse kidney tissue mass spectrum.

[0054] In the step (1), the mice are selected as Balb / c male mice, 7-8 weeks old.

[0055] In step (3), stand still for 15-25 minutes to make it spread evenly.

[0056] In step (4), the corundum rod adhered with the mouse kidney tissue sl...

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Abstract

The invention discloses a biological tissue mass spectrometry imaging method which comprises the following steps: (1) selecting in-vitro kidney tissues of a mouse, and slicing after freezing, thereby obtaining mouse kidney tissue slices; (2) selecting a corundum rod, attaching the mouse kidney tissue slices obtained in the step (1) onto the corundum rod, and naturally airing; (3) dripping an acriflavine solution onto the mouse kidney tissue slices, and standing, so that the solution uniformly diffuses; and (4) placing the corundum rod adhered with the mouse kidney tissue slices on which the acriflavine solution drips in a laser mass spectrometer for detecting, and processing mass spectrometric data ionized after laser analysis by virtue of software, thereby obtaining the imaging mass spectrum of the acriflavine-containing mouse kidney tissue slices. The method is rapid and direct, matrix interference and complicated sample pretreatment processes can be avoided, mass spectrometry signals can be regulated in time and space by the laser analysis post-ionization imaging technology, and thus optimization of the imaging mass spectrum is facilitated.

Description

technical field [0001] The invention belongs to the technical field of mass spectrometry imaging, and in particular relates to a biological tissue mass spectrometry imaging method. Background technique [0002] Laser desorption post-ionization mass spectrometry has achieved great development in the last ten or twenty years. Compared with traditional MALDI mass spectrometry, it has the advantages of being fast, direct and simple, and does not require complicated sample pretreatment. Therefore, its application is gradually emerging. In the biological field, it includes the detection of small biological molecules such as short peptides and amino acids, drug molecules, chemical additives and other chemical molecules. [0003] Mass spectrometry is a new analytical technique for studying the distribution of molecules in biological cells, tissues and living animals. It can detect and record a spatial distribution of molecules in a special system without labeling. Mass spectrometry...

Claims

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Application Information

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IPC IPC(8): G01N27/62
CPCG01N27/628
Inventor 胡勇军陈家新陆桥
Owner SOUTH CHINA NORMAL UNIVERSITY
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