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Phagocytosing type bacterium and application thereof in reducing sludge

A phage and bacteria technology, applied in the field of microorganisms, can solve the problem of high cost of sludge treatment, and achieve the effects of improving biodegradability and resource utilization, reducing operating costs and saving investment.

Active Publication Date: 2016-09-28
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the cost of sludge treatment is high, accounting for about 25% to 40% of the operating cost of the sewage plant.

Method used

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  • Phagocytosing type bacterium and application thereof in reducing sludge
  • Phagocytosing type bacterium and application thereof in reducing sludge
  • Phagocytosing type bacterium and application thereof in reducing sludge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: Separation and purification of phage type bacteria

[0036] The phage-type bacteria in the present invention are separated from the activated sludge of the secondary sedimentation tank of a sewage treatment plant in Jiangsu, and the specific process is as follows:

[0037]1) Isolation and identification of host bacteria: Take samples from the activated sludge of the secondary sedimentation tank of the municipal sewage treatment plant for preliminary treatment of the sludge. The sludge supernatant was diluted to 10 with Bacterial Phosphate Buffer 5 ~10 7 times, and then take 200 μL of each serial dilution and spread it on the peptone yeast extract solid medium. After the coating is completed, let it stand for 20 minutes, and then move it to a constant temperature incubator at 28° C. for upside-down cultivation. After culturing for 12-18 hours, streak and separate the isolated colonies that appear on the plate to obtain candidate host bacteria, and finall...

Embodiment 2

[0043] Embodiment 2: the gene identification of the 16S rRNA of SDWB01 bacterial strain

[0044] The 16S rRNA gene sequencing method was used to classify and identify the strain SDWB01, and the specific steps were as follows:

[0045] Sample DNA preparation: routine bacterial DNA extraction method was used for analysis.

[0046] PCR Primers: Use the following primers:

[0047] Upstream primer 1 (63F): 5'-CAGG CCTAACACATGCAAGTC-3'

[0048] Downstream primer 2 (Bdg842R): 5'-CGWCACTGAAGGGGTCAA-3'

[0049] PCR reaction system: 25 μL reaction system, reaction solution composition: 3 μL DNA template, 2 μL upstream primer, 2 μL downstream primer, 12.5 μL Taq enzyme and its mixture, and make up to 25 μL with ultrapure water.

[0050] PCR reaction conditions: pre-denaturation at 94°C for 3 min; main cycle of denaturation at 94°C for 1 min, annealing at 56°C for 45 s, extension at 72°C for 1 min, a total of 30 cycles; final extension at 72°C for 10 min. After the PCR reaction, the P...

Embodiment 3

[0053] Embodiment 3: Bacterial strain SDWB01 is to the phagocytosis of Gram-negative bacteria in sludge

[0054] 1) Separation, purification and screening of gram-negative bacteria: take samples from the activated sludge of the secondary sedimentation tank of the municipal sewage treatment plant and conduct preliminary treatment of the sludge. 20min, then the sludge supernatant was serially diluted to 10 with sterile phosphate buffer 5 ~10 7 times. The bacterium in the diluted sludge supernatant is coated and isolated for culture, and the specific steps are: take 200 μL of each gradient dilution solution and spread it on the solid medium of peptone yeast extract. After the coating is completed, let it stand for 20 minutes, and then move it to a constant temperature incubator at 28° C. for upside-down cultivation. Isolate and purify the strain obtained by coating and separating the above-mentioned solid medium with peptone yeast extract. The specific steps are: after the abo...

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Abstract

The invention discloses a phagocytosing type bacterium. The phagocytosing type bacterium is identified to be a bdellovibrio organism, called bdellovibrio for short and classified and named as Bdellovibrio sp., the strain is preserved in the China General Microbiological Culture Collection Center on May 12th, 2016, and the preservation serial number is CGMCC No.11671. The invention further discloses application of the phagocytosing type bacterium in reducing sludge, and further discloses a bacterium extract. The strain is obtained through screening in sludge of a secondary sedimentation tank of a municipal wastewater treatment plant and meets the biosafety regulations; the strain serves as the phagocytosing type bacterium to be applied in sludge cell disruption dewatering, and the sludge dewatering and reducing performance is greatly improved by phagocytosing suspension bacteria and splitting sludge flocs, so that the operation cost of the wastewater treatment plant is reduced, both ecological safety and sanitary safety are considered, and the ecological civilization construction of the environment is promoted.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a phage-type bacterium and its application in sludge reduction. Background technique [0002] More than 80% of the world's sewage treatment plants use the activated sludge method to purify sewage, but at the same time, a large amount of residual sludge with a water content of 0.15-1% of the treated water volume is as high as 95%. At present, the cost of sludge treatment is high, accounting for about 25% to 40% of the operating costs of sewage plants. Therefore, the treatment and disposal of municipal sludge has become an environmental issue of common concern in the world, and various investigations and statistical data at home and abroad show that improving sludge dewatering performance has become the key to effective sludge treatment and disposal. The residual sludge produced by the municipal sewage biological treatment process is also called municipal sludge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/01
CPCC02F11/02C02F2303/06C12N1/20C12N1/205C12R2001/01
Inventor 余冉陈舟凯张诗文
Owner SOUTHEAST UNIV
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