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Acellular matrix material based on adipose-derived stem cell sheet and preparation method of acellular matrix material

A technology of acellular matrix and cell membrane, which is applied in the field of acellular matrix material based on adipose stem cell membrane and its preparation, and achieves the effects of uniform texture, good traction resistance and simple preparation method

Active Publication Date: 2016-09-28
SHANGHAI SIXTH PEOPLES HOSPITAL
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Problems solved by technology

Currently commonly used decellularization methods include permeation solution method, detergent method, enzyme digestion method, cell freeze-thaw method, etc., and no one method is suitable for all types of decellularized materials

Method used

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  • Acellular matrix material based on adipose-derived stem cell sheet and preparation method of acellular matrix material
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  • Acellular matrix material based on adipose-derived stem cell sheet and preparation method of acellular matrix material

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Embodiment Construction

[0024] The present invention will be further described below in conjunction with specific embodiments with reference to the accompanying drawings, so as to better understand the present invention.

[0025] 1. Primary culture of adipose-derived stem cells

[0026] Beagle dogs, 6-8 months old, after pentobarbital general anesthesia, aseptically cut 10g of fat tissue in the groin, removed the visible fascia and blood vessels, soaked in 0.25% chloramphenicol for 30 minutes, rinsed with PBS 3 times, ophthalmic scissors Cut it into pieces as much as possible, add 0.1% type Ⅰ collagenase to digest at 37°C for 1 hour, filter through a 100-mesh filter, centrifuge at 300g for 5 minutes, add stem cell medium to resuspend, inoculate in a 100mm cell culture dish and continue to cultivate, every 2-3 days to change the medium.

[0027] Composition of adipose stem cell medium: 450ml low-sugar DMEM medium + 50ml fetal bovine serum + 100U / ml penicillin + 100mg / ml streptomycin sulfate, steriliz...

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Abstract

The invention provides an acellular matrix material based on an adipose-derived stem cell sheet and a preparation method of the acellular matrix material. The acellular matrix material is an autologous adipose-derived stem cell sheet. According to the acellular matrix material, vitamin C is utilized to promote formation of the adipose-derived stem cell sheet, then the repeated freezing and thawing method and Triton*100 acellular treatment are used together to structure the acellular matrix material based on the adipose-derived stem cell sheet. The acellular matrix material is even in surface, uniform and homogeneous in texture and rich in content of extracellular matrix, forms a porous three-dimensional tissue, has better tensile property, low probability of breakage, no immunogenicity, capability of auto-transplantation, and operability of clinical application, is simple in preparation method and has a very high practical value.

Description

technical field [0001] The invention relates to the field of tissue engineering and regenerative medicine, in particular to an acellular matrix material based on adipose stem cell membrane and a preparation method thereof. Background technique [0002] Severe soft tissue defects can be caused by tumor surgery, trauma, or congenital defects. Common clinical repair methods include artificial material filling and autologous tissue flap transplantation, but these methods have limitations such as poor histocompatibility, large trauma, and necrotic absorption of the graft. With the development of tissue engineering technology, tissue engineering is expected to become a new and effective repair method. [0003] Cell scaffold is one of the main research contents of tissue engineering, which plays a role in supporting seed cells and guiding tissue regeneration. An ideal scaffold material should have non-immunogenicity, strong plasticity, and good biocompatibility. So far, no ideal c...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/56
CPCA61L27/3604A61L27/3687A61L27/3691A61L27/56A61L2430/34
Inventor 傅强周术奎
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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