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Pollution prevention and control method for ethanol consumption bacteria in ethanol producing genetic engineering cyanobacteria culture system

A culture system and genetic engineering technology, applied in the field of pollution prevention and control for ethanol-consuming bacteria, can solve problems such as ethanol bio-pollution, achieve the effect of improving carbon source capture efficiency and reducing carbon source capture costs

Active Publication Date: 2016-09-21
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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AI Technical Summary

Problems solved by technology

[0005] The invention aims to solve the technical problem of biological pollution of ethanol consumption product in the process of producing ethanol by ethanol-producing genetically engineered cyanobacteria; it provides a method for preventing and controlling the pollution of ethanol-consuming bacteria in the ethanol-producing genetically engineered cyanobacteria culture system

Method used

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  • Pollution prevention and control method for ethanol consumption bacteria in ethanol producing genetic engineering cyanobacteria culture system
  • Pollution prevention and control method for ethanol consumption bacteria in ethanol producing genetic engineering cyanobacteria culture system
  • Pollution prevention and control method for ethanol consumption bacteria in ethanol producing genetic engineering cyanobacteria culture system

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Embodiment 1

[0016] Example 1: In this embodiment, the pollution control method of ethanol-consuming bacteria in the ethanol-producing genetically engineered cyanobacteria culture system is applied in an indoor glass column photoreactor.

[0017] The specific method is to add 600ml of BG11 medium with an initial sodium bicarbonate concentration of 180mmol / L in an 800ml glass column photoreactor, and directly insert the genetically engineered Synechocystis sp. -2 the s -1 , cultured at 30°C, the pH value in the culture solution gradually increased to 11.0 as the algae cells grew with sodium bicarbonate, and CO 2 The gas adjusted the pH value to maintain at 11.0±0.5, and the product ethanol gradually accumulated while the algae cells grew.

[0018] The genetically engineered cyanobacteria is an ethanol-producing mutant strain of Synechocystis sp. PCC 6803, which was published by Gao Zhengxu et al. in Energy&Environmental Science magazine in May 2012 (Gao ZX, Zhao H, Li ZM, Tan XM, Lu XF: P...

Embodiment 2

[0021] Example 2: In this embodiment, the pollution control method of ethanol-consuming bacteria in the ethanol-producing genetically engineered cyanobacteria culture system is applied in an outdoor film-hanging bag-type photoreactor.

[0022] In an 8L film hanging bag photoreactor, add 6L tap water to prepare a BG11 medium with an initial sodium bicarbonate concentration of 180mmol / L without high temperature sterilization, and insert genetically engineered cyanobacteria, under the conditions of outdoor natural light intensity and temperature After culturing, the pH value in the culture solution gradually increased to 11.0 as the algae cells grew using sodium bicarbonate as a carbon source, and CO 2 The gas adjusted the pH value to maintain at 11.0±0.5, and the product ethanol gradually accumulated while the algae cells grew.

[0023] The genetically engineered cyanobacteria are the same as in Example 1.

[0024] at 5% CO 2 Conditions are control experiments. The culture co...

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Abstract

The invention discloses a pollution prevention and control method for ethanol consumption bacteria in an ethanol producing genetic engineering cyanobacteria culture system and belongs to the technical field of biological energy and the technical field of algal culture. The method mainly solves the technical problem about biological pollution prevention and control over the ethanol consumption bacteria in the genetic engineering cyanobacteria ethanol production process. The method comprises the steps in the ethanol producing genetic engineering cyanobacteria culture process, through a high-pH strategy that the culture system is captured through a sodium bicarbonate carbon source, and the pH value is controlled to gradually rise to 11.0 and kept at 11.0+ / -0.5 in the culture process, breeding of the ethanol consumption bacteria and ethanol consumption in the culture process are inhibited. The method can provide reference for a large-scale cyanobacteria culture pollution control system, and has important significance on biology liquid fuel produced from genetic engineering cyanobacteria.

Description

technical field [0001] The invention belongs to the technical field of bioenergy and algae breeding technology, and specifically relates to a method for preventing and controlling pollution of ethanol-consuming bacteria in an ethanol-producing genetic engineering cyanobacteria culture system. Background technique [0002] Global environmental pollution and potential energy crisis make it more urgent and important to develop sustainable green energy to supplement or replace the increasingly depleted fossil energy. As the earliest commercialized bio-liquid fuel, bioethanol is widely accepted and used as a substitute or auxiliary product for gasoline or petroleum due to its high octane number, high heat of vaporization, and low vapor pressure (Thangavelu, S.K., A. Ahmed, and F.N.Ani, Review on bioethanol as alternative fuel for spark ignition engines. Renewable & Sustainable Energy Reviews, 2016.56: p.820-835). At present, the vast majority of bioethanol produced industrially ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12R1/01
CPCC12P7/06Y02E50/10
Inventor 吕雪峰朱至谈晓明栾国栋
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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